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All Journal HAYATI Journal of Biosciences Science and Technology Indonesia Indonesian Journal of Applied Research (IJAR)
Zaelani, Bella Fatima Dora
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Earth & Planetary Sciences Environmental Science Materials Science & Nanotechnology Physics

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Myristicin Inhibit Invasion and Migration of Melanoma Cells through Suppression of MMP2 and MMP9 Gene Expression Krisnayanti, Ni Putu Eka; Mariya, Silmi; Indriawati, Iin; Dayana, Hepy; Zaelani, Bella Fatima Dora; Tjandrawinata, Raymond R.; Darusman, Huda Shalahudin
Science and Technology Indonesia Vol. 9 No. 4 (2024): October
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.4.884-892

Abstract

Melanoma is the deadliest type of skin cancer, having a high mortality rate. This cancer has an aggressive nature, is highly invasive, and has the tendency to metastasize. Matrix metalloproteinases (MMPs) are essential in that process, especially MMP2 and MMP9. Their expression is upregulated during metastasis progression. Myristicin is one example of a compound that can be utilized to target MMP 2 and MMP 9 in melanoma. This research concerns the activity of myristicin to inhibit melanoma cell invasion and migration by suppressing MMP2 and MMP9 gene expression. The MTT assay in this study demonstrated that myristicin exhibited strong cytotoxic activity against melanoma cells. This compound works in a dose-dependent manner by inhibiting cell migration and invasion. The invasion test was performed using the transwell assay, whereas the migration test was performed using the wound healing assay. The invasion assay results were consistent with MMP2 and MMP9 gene expression. These two genes were analyzed using the RT-qPCR technique. It has been demonstrated that low gene expression in melanoma cells inhibits cell invasion. In contrast, higher MMP2 and MMP9 gene expression was associated with an increase in the number of invasive cells on average. However, MMP2 and MMP9 in excessive expression and uncontrolled activity impair the ability of melanoma cells to form a monolayer sheet to cover wound gaps. This condition significantly reduced the migration rate and percentage of wound closure.
3D Culture Cells Technique for Exosomes Isolation of HEK293 and its Application on WiDr Cells Audina, Mia; Mariya, Silmi; Zaelani, Bella Fatima Dora; Yuliana; Darusman, Huda Shalahudin
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1173-1180

Abstract

Three-dimensional (3D) culture is a technique commonly utilized in bioprocessing and biomedical research. Exosomes have been investigated as carriers for medications in numerous studies employing 3D culture methodologies. The objective of this research is to employ 3D cell culture for the isolation and treatment of exosomes targeting colon cancer cells. The isolation of exosomes obtained from HEK293 cells was conducted through the ultracentrifugation technique. Subsequently, exosome treatment was administered to WiDr cells at concentrations of 3.5 µg/ml, 7 µg/ml, and 14 µg/ml.The validation of molecular markers of exosomes (CD9 and CD81), along with BAX, BCL-2, and CD133, was performed using qRT-PCR. The findings revealed the successful isolation of exosomes derived from HEK293 cells, which exhibited the expression of markers CD9 and CD81. Furthermore, the expression of BAX and BCL-2 indicated the potential of exosomes to induce apoptosis, while the expression level of CD133 decreased with treatment at varying concentrations. These results suggest that exosome treatment has the capability to impede the proliferation of WiDr cells and reduce the expression of CD133, thereby signifying the potential application of exosomes as an in-vitro model for investigating cancer therapy in the future.
In Vitro Study of Piper Crocatum Ruiz & Pav Potency as HMG-CoA Reductase and Malondialdehyde Formation Inhibitor Zaelani, Bella Fatima Dora; Safithri, Mega; Andrianto, Dimas
Indonesian Journal of Applied Research (IJAR) Vol. 5 No. 3 (2024): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v5i3.504

Abstract

Coronary heart disease is a serious health problem and it is known to be lethal. The disease can be triggered by the accumulation of cholesterol plaques in the walls of blood vessels This cholesterol formation is assisted by methylglutaril-CoA reductase (HMG-CoA reductase) enzyme. Red betel contains active compounds that have the potential to act as HMG-CoA reductase inhibitor. This study aimed to predict the potential of ethanol extract compounds, water fraction, ethyl acetate fraction, and red betel n-hexane fraction as HMG-CoA reductase inhibitors by in vitro method. The in vitro method which consisted of extraction by maceration, graded fractionation with n-hexane, ethyl acetate, and water, continued by thiobarbituric acid (TBA) method to see the potential antioxidant activity of the sample. The Result showed that the highest inhibition of HMG-CoA reductase is 94% was found in the concentration 200 ppm ethyl acetate fraction. Also antioxidant activity of red betel showed by the inhibition of malondialdehyde formation had the highest activity in the ethyl acetate fraction than other fractions used.
Co-Authors Dayana, Hepy DIMAS ANDRIANTO Huda Shalahudin Darusman Indriawati, Iin Krisnayanti, Ni Putu Eka Mega Safithri Mia Audina Raymond R. Tjandrawinata SILMI MARIYA Yuliana Yuliana