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All Journal HAYATI Journal of Biosciences Hippocampus: Jurnal Pengabdian Kepada Masyarakat
Mia Audina
Universitas Muhammadiyah Pontianak
Agriculture, Biological Sciences & Forestry Humanities Earth & Planetary Sciences Environmental Science Social Sciences Other

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3D Culture Cells Technique for Exosomes Isolation of HEK293 and its Application on WiDr Cells Audina, Mia; Mariya, Silmi; Zaelani, Bella Fatima Dora; Yuliana; Darusman, Huda Shalahudin
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1173-1180

Abstract

Three-dimensional (3D) culture is a technique commonly utilized in bioprocessing and biomedical research. Exosomes have been investigated as carriers for medications in numerous studies employing 3D culture methodologies. The objective of this research is to employ 3D cell culture for the isolation and treatment of exosomes targeting colon cancer cells. The isolation of exosomes obtained from HEK293 cells was conducted through the ultracentrifugation technique. Subsequently, exosome treatment was administered to WiDr cells at concentrations of 3.5 µg/ml, 7 µg/ml, and 14 µg/ml.The validation of molecular markers of exosomes (CD9 and CD81), along with BAX, BCL-2, and CD133, was performed using qRT-PCR. The findings revealed the successful isolation of exosomes derived from HEK293 cells, which exhibited the expression of markers CD9 and CD81. Furthermore, the expression of BAX and BCL-2 indicated the potential of exosomes to induce apoptosis, while the expression level of CD133 decreased with treatment at varying concentrations. These results suggest that exosome treatment has the capability to impede the proliferation of WiDr cells and reduce the expression of CD133, thereby signifying the potential application of exosomes as an in-vitro model for investigating cancer therapy in the future.
Meminimalisir Sampah Plastik Melalui Pemanfaatan Botol Bekas Sebagai Akuarium Perikanan di SD Negeri 22 Seladu Saputri, Dwi; Kusumawardani, Sagita; Febri, Febri; Yayan, Krisentia; Lugeri, Leo; Hanavi, Muhammad Ilham; Agam, Beryaldi; Urabi, Debby; Haq, Izhar Amirul; Jordi, Jordi; Kristiandi, Kiki; Sigiro, Oktavia Nurmawaty; Agusti, Evelin; Mahgfirah, Indria; Audina, Mia; Sangkala, Sangkala; Masara'T, Sudirman
Hippocampus: Jurnal Pengabdian Kepada Masyarakat Vol 4 No 1 (2025): Juni 2025
Publisher : PUSAT PENELITIAN DAN PENGABDIAN PADA MASYARAKAT (PPPM) POLITEKNIK NEGERI SAMBAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47767/hippocampus.v4i1.1070

Abstract

Plastic waste is a serious environmental problem that requires immediate handling, especially in educational environments. SD Negeri 22 Seladu faces the challenge of managing plastic waste, especially used bottles, which have not been utilized optimally. This condition shows the need to increase students' awareness of plastic waste management and the provision of environmentally based learning media. This service activity aims to minimize plastic waste, increase students' understanding of waste management, and create learning media in the form of aquariums from used bottles. The method used includes preparation stages, delivery of materials, and practice of making aquariums from used plastic bottles. Activities are carried out with a participatory approach to actively involve students. The results of the activity show that students are able to understand the concept of recycling, increase creativity, and have a higher awareness of the importance of protecting the environment. This program provides significant educational and environmental benefits. By using used bottles as a learning medium, this activity can be used as a model for environmental education in other schools to support sustainable plastic waste management.
Co-Authors Agam, Beryaldi Agusti, Evelin Febri Febri Hanavi, Muhammad Ilham Haq, Izhar Amirul Huda Shalahudin Darusman Jordi, Jordi Kristiandi, Kiki Kusumawardani, Sagita Lugeri, Leo Mahgfirah, Indria Masara'T, Sudirman Sangkala Saputri, Dwi Sigiro, Oktavia Nurmawaty SILMI MARIYA Urabi, Debby Yayan, Krisentia Yuliana Yuliana Zaelani, Bella Fatima Dora