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All Journal HAYATI Journal of Biosciences JURNAL BIOEDUCATION
Mia Audina
Universitas Muhammadiyah Pontianak
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Education

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PENGEMBANGAN MEDIA PEMBELAJARAN INTERAKTIF BERBASIS FLASH MATERI SEL KELAS XI DI SMAS KEMALA BHAYANGKARI Mia Audina; Anandita Eka Setiadi; Adi Pasah Kahar
JURNAL BIOEDUCATION Vol 6, No 2 (2019): Jurnal Bioeducation
Publisher : Universitas Muhammadiyah Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (712.132 KB) | DOI: 10.29406/.v6i2.1167

Abstract

Media pembelajaran yang digunakan di SMAS Kemala Bhayangkari pada materi sel menarik tetapi masih tidak interaktif. Penelitian ini bertujuan untuk mengembangkan media pembelajaran interaktif berbasis flashmateri sel yang layak digunakan. Pengembangan media pembelajaran interaktif berbasis flash ini menggunakan metode penelitian dan pengembangan (R&D) model 4-D, yaitu tahap Define (Pendefinisian), Design (Perancangan), Develop (Pengembangan) dan Disseminate (Penyebaran). Pada tahap Disseminate (Penyebaran) tidak dilakukan. Hasil penelitian menunjukkan kevalidan pada aspek materi sebesar 83,33% (sangat valid), bahasa sebesar 85 % (sangat valid), dan aspek media sebesar 80,42 % (sangat valid). Berdasarkan uji coba skala kecil dan uji coba skala besar, respon siswa dan respon guru terhadap media pembelajaran interaktif berbasis flash baik, sehingga media dapat digunakan dalam proses pembelajaran. Keefektifan media pembelajaran diperoleh berdasarkan nilai postest. Ketuntasan klasikal kelas 91,42%, yang menunjukkan bahwa media pembelajaran interaktif berbasis flash materi sel dinyatakan efektif. Berdasarkan hasil yang diperoleh, menunjukkan bahwa media pembelajaran interaktif berbasis flash layak digunakan untuk siswa dalam pembelajaran biologi materi sel.
3D Culture Cells Technique for Exosomes Isolation of HEK293 and its Application on WiDr Cells Audina, Mia; Mariya, Silmi; Zaelani, Bella Fatima Dora; Yuliana; Darusman, Huda Shalahudin
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1173-1180

Abstract

Three-dimensional (3D) culture is a technique commonly utilized in bioprocessing and biomedical research. Exosomes have been investigated as carriers for medications in numerous studies employing 3D culture methodologies. The objective of this research is to employ 3D cell culture for the isolation and treatment of exosomes targeting colon cancer cells. The isolation of exosomes obtained from HEK293 cells was conducted through the ultracentrifugation technique. Subsequently, exosome treatment was administered to WiDr cells at concentrations of 3.5 µg/ml, 7 µg/ml, and 14 µg/ml.The validation of molecular markers of exosomes (CD9 and CD81), along with BAX, BCL-2, and CD133, was performed using qRT-PCR. The findings revealed the successful isolation of exosomes derived from HEK293 cells, which exhibited the expression of markers CD9 and CD81. Furthermore, the expression of BAX and BCL-2 indicated the potential of exosomes to induce apoptosis, while the expression level of CD133 decreased with treatment at varying concentrations. These results suggest that exosome treatment has the capability to impede the proliferation of WiDr cells and reduce the expression of CD133, thereby signifying the potential application of exosomes as an in-vitro model for investigating cancer therapy in the future.
Co-Authors Adi Pasah Kahar Anandita Eka Setiadi Huda Shalahudin Darusman SILMI MARIYA Yuliana Yuliana Zaelani, Bella Fatima Dora