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All Journal JURNAL KIMIA SAINS DAN APLIKASI Jurnal Ilmu Dasar Chimica et Natura Acta Martabe : Jurnal Pengabdian Kepada Masyarakat JKPK (Jurnal Kimia dan Pendidikan Kimia) Hydrogen: Jurnal Kependidikan Kimia Jurnal Pijar MIPA International Journal of Current Educational Research Journal of The Indonesian Society of Integrated Chemistry Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Folia Medica Indonesiana
Sutoyo, Suyatno
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Education Engineering Environmental Science Health Professions Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Social Sciences

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Green Synthesis of Copper Nanoparticles Using Gynura procumbens Leaf Extract as a Bioreductant and Their Antibacterial Activity Against Escherichia coli Rivana, Nancy; Sutoyo, Suyatno; Arizandy, Radita Yuniar; Setyarini, Wahyu
JKPK (Jurnal Kimia dan Pendidikan Kimia) Vol 11, No 1 (2026): JKPK (Jurnal Kimia dan Pendidikan Kimia)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jkpk.v11i1.116736

Abstract

Copper nanoparticles (CuNPs) are nanomaterials extensively utilized in optics, electronics, biology, catalysis, and as antibacterial agents. Sustainable synthesis approaches employing plant-derived bioreductants have garnered significant attention. In this study, CuNPs were synthesized via green chemistry using Gynura procumbens leaf extract as a bioreductant. Synthesis was performed by combining the leaf extract with 0.1 M CuSO₄ solution at volume ratios of 1:1, 1:2, 1:3, and 1:4. The mixture with the optimal volume ratio was further optimized at pH values of 8, 9, 10, and 11. The resulting CuNPs were characterized by UV-Vis spectroscopy, FTIR, and PSA. Antibacterial activity against Escherichia coli (ATCC 25922) was evaluated using the disk diffusion method. Optimal synthesis was achieved at a 1:2 volume ratio and pH 8. The CuNPs exhibited a maximum UV-Vis absorption at 316 nm, an average particle size of 8.23 nm, and a polydispersity index of 0.1631. FTIR spectra confirmed Cu–O bond vibrations at 457.64 and 617.14 cm-1. The synthesized CuNPs demonstrated strong antibacterial activity against E. coli, with an inhibition zone diameter of 13.89 ± 0.45 mm. These findings suggest that CuNPs synthesized via green methods using G. procumbens extract as a bioreductant have potential as antibacterial agents, particularly against E. coli.
Co-Authors Albahi, Fairuz Hammad Dewi Saputri, Ratih Evawati, Kholif Faricha, Yeni Askiyatul Fatma Listiandari, Intan Hasanah Jusman, Nur I Nyoman Suardana Khoirunnisa, Aisy Nita Kusumawati Nuniek Herdyastuti Nurrohman Sidiq, Muhammad Putri Sarini Putri, Retno Wahdany Huda Radita Yuniar Arizandy Rivana, Nancy Rudi Setiawan, Ahmad sabila, fauzia indah Salma, Nadiah Armadanti Sari Edi Cahyaningrum Setyarini, Wahyu Sofyah, Titik Mardiyanti TUKIRAN Tukiran Tukiran Wahono Widodo Yulianti, Dimas Ayu