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All Journal HAYATI Journal of Biosciences KONSELING RELIGI
Fanany, Rizal
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Religion Agriculture, Biological Sciences & Forestry Arts Humanities Earth & Planetary Sciences

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The Portrait of Pseudo Happiness on Social Media: Formulation of Happiness Literacy Concept Based on Quran to Realize Authentic Happiness in Industry 4.0 Hanafi, Yusuf; Rifai, Mochamad Khoirul; Fanany, Rizal; Ikhsan, M. Alifudin; Diyana, Tsania Nur
KONSELING RELIGI Vol 12, No 1 (2021): Juni 2021
Publisher : Sekolah Tinggi Agama Islam Negeri (STAIN) Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21043/kr.v12i1.7055

Abstract

Recently, the world experiences a striking process of modernization that provokes dehumanization phenomena. Consequently, modern society encounters a spiritual crisis (azmah ruhaniyah), primarily caused by greediness rationalism mind-set as the consequence of technology hegemony. Particularly, in the industry 4.0 revolution era, its prevalent challenge is the appearance of disruptive technology that affects almost every aspect of life, including the spiritual aspect. This spiritual crisis affects the formation of soul-sick (qalbun saqim). One of the examples is the pseudo happiness that has become an epidemic in various kinds of social media like WhatsApp (WA), Instagram (IG), Facebook (FB), Twitter, and so forth. Social media users share happiness content without being accompanied by their real emotions. These emotions are illustrated as pseudo happiness, which is the fake happiness acknowledged by the people. In this study, the researchers formulated happiness literacy by using the detection and education method to realize authentic happiness. The detection of pseudo happiness refers to the indicators built based on the universal values of the Quran. This idea is presented on a website named literasibahagia.or.id to ease the access of technologically literate users. The findings of this study unravel the decreasing pseudo happiness on social media, along with society's local culture usage on social media
Cloning and Optimized Expression of Bst DNA Polymerase from Geobacillus stearothermophillus in Escherichia coli BL21 Taufik, Intan; Fanany, Rizal; Manjaswari, Agika; Pratama, Fenryco
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.155-163

Abstract

Bst DNA polymerase possesses strand displacement activity, enabling isothermal DNA amplification without requiring a thermal cycler. This enzyme is utilized in the Loop-Mediated Isothermal Amplification (LAMP) method, which offers advantages in speed and simplicity over Polymerase Chain Reaction (PCR) method. The growing demand for Bst DNA polymerase highlights the need for cost-effective in-house production, as a commercial option is economically challenging. For that purpose, this study aims to construct and optimize the expression of Bst DNA polymerase from Geobacillus stearothermophilus in Escherichia coli. The expression constructs pET16b.BstHF vector was constructed using Gibson Assembly and expressed in E. coli BL21 (DE3). Optimal expression was achieved with 1 mM IPTG, induction at OD600 0.8 and 6-hour induction time. The purified enzyme was achieved with a protein yield of 2,175 mg/L culture and demonstrated effective polymerase activity for LAMP.
Co-Authors FENRYCO PRATAMA Ikhsan, M. Alifudin Manjaswari, Agika Rifai, Mochamad Khoirul Taufik, Intan Tsania Nur Diyana Yusuf Hanafi