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Cloning and Optimized Expression of Bst DNA Polymerase from Geobacillus stearothermophillus in Escherichia coli BL21 Taufik, Intan; Fanany, Rizal; Manjaswari, Agika; Pratama, Fenryco
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.155-163

Bst DNA polymerase possesses strand displacement activity, enabling isothermal DNA amplification without requiring a thermal cycler. This enzyme is utilized in the Loop-Mediated Isothermal Amplification (LAMP) method, which offers advantages in speed and simplicity over Polymerase Chain Reaction (PCR) method. The growing demand for Bst DNA polymerase highlights the need for cost-effective in-house production, as a commercial option is economically challenging. For that purpose, this study aims to construct and optimize the expression of Bst DNA polymerase from Geobacillus stearothermophilus in Escherichia coli. The expression constructs pET16b.BstHF vector was constructed using Gibson Assembly and expressed in E. coli BL21 (DE3). Optimal expression was achieved with 1 mM IPTG, induction at OD600 0.8 and 6-hour induction time. The purified enzyme was achieved with a protein yield of 2,175 mg/L culture and demonstrated effective polymerase activity for LAMP.

Equality-Based Inclusive and Transformational Leadership Style Gender: Study of Khofifah Indar Parawansa as Governor of East Java Rachmadini, Anisa; Aprilia, Syffa; Devita, Audya Shalma Alfia; Setiadi, Yurika; Taufik, Intan; Gunawan, Muhamad Farel Alfarizy
Majapahit Journal of Islamic Finance and Management Vol. 5 No. 4 (2025): Islamic Finance and Management
Publisher : Universitas KH. Abdul Chalim Mojokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31538/mjifm.v5i4.547

This study aims to analyze the inclusive and transformational leadership styles based on gender equality implemented by Khofifah Indar Parawansa in her leadership as the Governor of East Java for the 2025–2030 period. Amid the dynamics of regional development that demand the participation of all societal groups, leadership styles that are responsive to gender equality issues are essential to ensure the realization of social justice and equitable welfare. Using a descriptive qualitative approach through literature review and documentation methods, this article examines the characteristics of Khofifah's leadership, which emphasizes openness, participation, and empowerment of vulnerable groups, especially women. The analysis reveals that Khofifah implemented innovative policies such as the Gender-Responsive Development Planning Forum (Musrenbang Gender), the “Women- and Child-Friendly Village” program, and “Jatim Puspa” (East Java Women's Business Empowerment), which have proven effective in increasing women's participation across various sectors. Additionally, her transformational leadership style is reflected in the strategic program "Nawa Bhakti Satya," aimed at building a more inclusive, just, and sustainable social system. This study concludes that the combination of inclusive and transformational leadership based on gender equality significantly contributes to creating effective, responsive, and equitable regional governance.

Equality-Based Inclusive and Transformational Leadership Style Gender: Study of Khofifah Indar Parawansa as Governor of East Java Rachmadini, Anisa; Aprilia, Syffa; Devita, Audya Shalma Alfia; Setiadi, Yurika; Taufik, Intan; Gunawan, Muhamad Farel Alfarizy
Majapahit Journal of Islamic Finance and Management Vol. 5 No. 4 (2025): Islamic Finance and Management
Publisher : Universitas KH. Abdul Chalim Mojokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31538/mjifm.v5i4.547

This study aims to analyze the inclusive and transformational leadership styles based on gender equality implemented by Khofifah Indar Parawansa in her leadership as the Governor of East Java for the 2025–2030 period. Amid the dynamics of regional development that demand the participation of all societal groups, leadership styles that are responsive to gender equality issues are essential to ensure the realization of social justice and equitable welfare. Using a descriptive qualitative approach through literature review and documentation methods, this article examines the characteristics of Khofifah's leadership, which emphasizes openness, participation, and empowerment of vulnerable groups, especially women. The analysis reveals that Khofifah implemented innovative policies such as the Gender-Responsive Development Planning Forum (Musrenbang Gender), the “Women- and Child-Friendly Village” program, and “Jatim Puspa” (East Java Women's Business Empowerment), which have proven effective in increasing women's participation across various sectors. Additionally, her transformational leadership style is reflected in the strategic program "Nawa Bhakti Satya," aimed at building a more inclusive, just, and sustainable social system. This study concludes that the combination of inclusive and transformational leadership based on gender equality significantly contributes to creating effective, responsive, and equitable regional governance.

Starter Culture Modulates Microbial Diversity During Wine-Coffee Fermentation: A DGGE-Based Molecular Study Nasir, Fitri Handayani; Astuti, Dea Indriani; Taufik, Intan; Fitriagustiani; Ikramullah, Muh. Chaeril; Putri, Nurul Febriani; Pasmawati
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i2.28269

Wine-flavored coffee is a unique post-harvest product characterized by fruity, acidic, and winey notes, highly favored by Indonesian consumers and possessing strong potential in the global market. However, maintaining consistency in flavor and quality remains a challenge due to the variability of natural fermentation. This study aimed to evaluate the impact of yeast and bacterial starter culture inoculation on microbial community dynamics during wine-coffee fermentation, using a molecular approach based on PCR-DGGE. DGGE analysis revealed that natural fermentation without inoculation involved diverse populations of bacteria, yeasts, and filamentous fungi, with eukaryotic microbes dominating from the early stages. Sequencing identified prevalent yeast genera including Pichia, Torulaspora, Hanseniaspora, Saccharomyces, and Candida, as well as Aspergillus among filamentous fungi. Bacterial communities were dominated by lactic acid bacteria and members of Lactobacillus, Klebsiella, Enterobacter, Pantoea, and Bacillus. In contrast, controlled fermentation with inoculated starter cultures (Pichia kudriavsevii and Klebsiella sp.) showed a more stable microbial profile throughout the process. Shannon-Wiener diversity indices demonstrated a significant difference (p = 0.05) between natural and inoculated fermentations, with species dominance observed in the latter. Cluster analysis confirmed that starter culture inoculation significantly influenced microbial succession and community structure. These findings highlight the importance of controlled fermentation using selected microbial starters to ensure consistent microbial ecology, which in turn contributes to reproducible quality in wine-flavored coffee. The molecular profiling approach provides valuable insights for improving fermentation practices and developing reliable starter culture formulations tailored to enhance flavor consistency and product quality.

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