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All Journal Jurnal Biogenerasi International Journal Of Science, Technology & Management (IJSTM) Healthcaring: Jurnal Ilmiah Kesehatan
Misnarliah
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Effect of Preparate Coloring Delay Achid Resistant Bacteria With Ziehl Neelsen Method On The Result of Microscopic Examination Misnarliah; Mudrika; Anastasia A Basir
International Journal of Science, Technology & Management Vol. 2 No. 2 (2021): March 2021
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v2i2.181

Abstract

The microscopic examination of smear from sputum specimens plays an important role in the initial diagnosis and monitoring of pulmonary TB treatment. The Ziehl Nelseen method is the method for examining acid-resistant bacteria smear recommended by WHO. This method has high specificity for detecting acid-resistant bacteria in sputum. This study aims to determine the effect of postponement of sputum smear staining on the quality of acid-resistant bacteria germs on the results of microscopic examination using the Ziehl Nelseen staining method. The length of time to delay staining used was 3 days, 2 days and the control was direct preparations staining. The sample used was positive smear sputum. The results showed that from 10 samples of sputum, the results obtained were directly stained with results of 1+ there were 7 samples, and 2+ there were 3 samples, while the samples with the preparations were delayed for 2 days and 3 days at room temperature, respectively +1 as many as 7 samples, and 2+ as many as 3 samples. However, there was no difference in the reading of the staining results between the direct stained samples with the stained preparations with a delay of 2 days and 3 days at room temperature. The results of this study still have many shortcomings and to improve them it is recommended to carry out further research with a larger scale and sample size.
Analysis Of Serum Cystatin C Levels In People With Type 2 Diabetes Mellitus As A Biomarker Of Early Detection Of Atherosclerosis Misnarliah; Anastasia A. Basir; Zainuddin
International Journal of Science, Technology & Management Vol. 2 No. 6 (2021): November 2021
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v2i6.370

Abstract

Type 2 diabetes mellitus (DMT2) is associated with atherosclerosis, which causes the disease. cardiovascular and increased mortality. It is still difficult to detect ateroskelerosis in the early stages. Arterial stenosis often develops without symptoms in patients. DMT2, then causes cardiovascular disease. Therefore, development diagnostics to easily detect early-stage atherosclerosis are needed. In this study, we focused on cystatin C serum, an inhibitor of cysteine proteinase. Some research results, it has reported a significant correlation between serum Cystatin C levels and Arterial stiffness in a group of normal individuals. Cystatin C serum has a correlation strong with a value of elasticity of the carotid artery walls that reflect the degree of atherosclerosis subclinical. This is a new sign that is quite potential as a biomarker of early detection atherosclerosis. The purpose of the study is to know the picture serum Cystatin C levels as an early marker to determine the presence of possible complications of atherosclerosis in DMT2 patients, as well as the usefulness of Cystatin-C in predicting atherosclerosis of the early stages. Research methods are analytical research with use cross-sectional design. The study was conducted by calculating the value of Cystatin C blood serum with DMT2. The study subjects were people with DMT2 in RSUD.Labuang Baji Makassarand its network. Sum the sample in this study was 20 people. Determination of the research subject is done by conducting a search on medical records of DMT2 patients who meet the criteria of inclusion and exclusion to achieve minimum number of samples. The study subjects were classified into two groups based on medical record data searches are subclinical atherosclerosis group and nonclinical group ateroskelorosis. The research sample is a serum sample. Serum sample examination is carried out in the Clinical Prodia Laboratory using PENIA method. The results of this study reported the results that Cystatin-C Serum is closely correlated with subclinical atherosclerosis (arterial stiffness. Increase in serum cystatin-C levels indicates the risk of atherosclerosis in Patients with DMT2. The results of this study reported that serum cystatin C levels in dmt2 patients in the nonclinical atherosclerosis group (n = 10) showed normal cystatin levels (0.50 - 0.96 mg/L), while serum Cystatin-C levels in dmt2 group patients showed normal cystatin levels (0.50 - 0.96 mg/L), while serum cystatin-C levels in DMT2 group patients Subclinical atherosclerosis (n = 10) has an increase in serum cystatin-C levels (> 0.90 mg/L). Level cystatin C serum is associated with SA in DMT2 patients. Cystatin C was identified as a predictor of atherosclerosis risk, after adjusting for a variety of factors associated with diabetes.
KARAKTERISTIK MORFOLOGI KOLONI BAKTERI LIMBAH BIOMEDIS CAIR RS UNHAS DENGAN METODE STREAK PLATE Wulandhani, Suci; Bida Purnamasari, Andi; Sunarti Mangol, Vivi; Misnarliah
Jurnal Biogenerasi Vol. 9 No. 1 (2024): Terbit volume 9 nomor 1 tahun 2024
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v9i1.3402

Abstract

Limbah biomedis terdiri dari semua buangan limbah baik cair maupun padat yang dihasilkan dari fasilitas dan aktivitas rumah sakit, sedangkan limbah biomedis cair merupakan hasil dari produksi atau kegiatan yang tidak dapat dipakai lagi berbentuk cair yang berasal dari pelayanan Kesehatan. Semua buangan yang berbentuk cair termasuk tinja mengandung mikroorganisme, bahan beracun dan radioaktif serta darah yang berbahaya bagi kesehatan. Limbah biomedis cair yang tidak dikelola dengan baik mengandung banyak bakteri yang dapat dijadikan sebagai salah satu indikator adanya pengolahan limbah yang tidak baik serta kondisi lingkungan yang tidak baik. Pemisahan bakteri diperlukan untuk mengetahui jenis, morfologi, fisiologi, karakteristiknya. Penelitian ini merupakan penelitian eksperimen laboratorik yang dianalisis secara deskriptif kualitatif yang bertujuan untuk mengisolasi dan menentukan karakteristik morfologi koloni bakteri limbah biomedis cair dengan metode streak plate. Sampel limbah biomedis cair berasal dari buangan akhir Rumah Sakit Pendidikan Universitas Hasanuddin. Hasil penelitian menunjukan bahwa dengan menggunakan metode streak plate bakteri dari limbah biomedis cair yang dikultur dan dipurifikasi diperoleh 7 isolat bakteri yang tumbuh pada media Nutrient Agar. Ketujuh isolat bakteri tersebut memiliki perbedaan karakteristik morfologi koloni yaitu 4 isolat memiliki warna putih keruh dan isolat lain berwarna putih dan putih kekuningan. 5 isolat dengan bentuk koloni bulat dengan tepi bergelombang (wavy) dengan elevasi cembung (convex).
PEWARNAAN GRAM ISOLAT BAKTERI DARI LIMBAH BIOMEDIS CAIR RUMAH SAKIT UNHAS DENGAN METODE ZIEHL NEELSEN Wulandhani, Suci; Wahyuni, Ayu; Hasyim, Ahmad; Misnarliah
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4386

Abstract

Hospitals as one of the health service institutions that provide inpatient, outpatient and emergency services, in their implementation can produce waste in solid, liquid or gas form which can pollute the environment. Hospital waste water is an accumulation of domestic waste and liquid biomedical waste with high levels of organic pollutants, so it can be treated biologically. Liquid biomedical waste contains many microorganisms where the presence of microorganisms, in this case bacteria, can be used as an indicator of waste management and unfavorable environmental conditions. One waste water treatment technology that is safe for the environment is using bacteria that have the potential to act as decomposers in the biodegradation process. To obtain bacteria that have the potential to act as natural decomposers, you can isolate the waste itself. The bacteria found in liquid biomedical waste make it possible to find a group of non-pathogenic hydrolytic bacteria where this group of bacteria can produce various enzymes that are capable of degrading liquid biomedical waste. Gram staining is a method to differentiate between types or spesies of bacteria namely gram-positive bacteria and gram-negative bacteria. The aim of this study was to identify bacteria using Ziehl Neelsen gram staining. The results of the research showed that there were two bacterial isolates that were gram negative, namely isolate codes UH1.1 and UH1.2 (bacillus) form characterized by the appearance of brick red cells and five isolates that were gram positive, namely isolate codes UH1.3, UH2.1 (bacillus) UH2.2, UH2.3 and UH3.1 (coccus form) are characterized by the appearance of purple cells, the difference in color is due to differences in the composition of the cell walls of each bacteria.
EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEH HIJAU TERHADAP BAKTERI MYCOBACTERIUM TUBERCULOSIS Misnarliah; Ernawati, Andi
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4431

Abstract

Tuberculosis is still a global health problem. Prolonged treatment of tuberculosis and using several anti-tuberculosis drugs (OAT) can cause side effects, one of which is multidrug resistance. Cases of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium strains continue to increase. . Research and development of active compounds from medicinal plants to achieve more effective tuberculosis treatment is still being promoted. This research aims to determine the potential and effectiveness of green tea plant (Camellia sinensis) leaf extract in inhibiting the growth of Mycobacterium tuberculosis strain H37Rv bacteria using the LJ (Lowenstein-Jensen) method in vitro. The research samples used were green tea plant leaves (Camellia sinensis) obtained from the Malino Village Tea Plantation, Tinggi Moncong Regency, Gowa District, South Sulawesi. The leaf extract was made in 4 types of concentrations, namely 10, 20, 50 and 100 µg/ml, each of which was tested against the clinical isolate of Mycobacterium tuberculosis strain H37Rv using the LJ (Lowenstein-Jensen) method as the standard for tuberculosis examination. Of the four extracts tested in vitro, only extract concentrations of 50 and 100 µg/ml were able to very strongly inhibit and kill the growth of Mycobacterium tuberculosis strain H37Rv (inhibition percentage of 100%), not a single bacterial colony growth was found during the observation period. The percentage of inhibition of green tea plant leaves (Camellia sinensis) is the same as the percentage of inhibition of the drug rifampicin. Thus, the leaves of the green tea plant (Camellia sinensis) with concentrations of 50 and 100 µg/ml have potential antituberculosis activity and are prospective to be developed as antituberculosis from natural ingredients, and also as an additional therapeutic complement for TB.
ISOLASI DAN IDENTIFIKASI BAKTERI INDIGEN YANG BERPOTENSI SEBAGAI AGEN BIOREMEDIASI DARI LIMBAH BIOMEDIS CAIR Wulandhani, Suci; Lohing, Ruben; Hasyim, Ahmad; Misnarliah
Healthcaring: Jurnal Ilmiah Kesehatan Vol. 2 No. 2 (2023): Vol : 2 No : 2 (2023 ) : Periode Juli 2023
Publisher : Information Technology and Science (ITScience)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47709/healthcaring.v2i2.2484

Abstract

Biomedical waste contains viruses, fungi, toxic chemicals, and radioactive materials. Biomedical waste is dangerous because it is infectious, causing health risks and easily contaminating other wastes if not treated properly. Bioremediation is a method that can be used to treat waste by utilizing indigenous bacteria to remove contaminants. The study aims to determine whether there are isolates of indigenous bacteria that have the potential to be bioremediation agents for liquid biomedical waste in hospitals. The sample comes from a liquid biomedical waste disposal tank at Hasanuddin University Hospital, Makassar. Isolation of bacteria used the pouring method, while identification used the Gram Ziehl Neelson staining method. Pathogenesis was carried out by testing the ability of lactose fermentation and the ability of blood hemolysis. Parameters observed were the morphological and microscopic characteristics of the bacterial isolates, as well as their lactose fermentation ability and hemolytic ability. The results showed that there was one indigenous bacterial isolate from the liquid biomedical waste of Hasanuddin University Hospital Makassar that had the ability to ferment lactose but was unable to hemolyze blood. So that the indigenous bacterial isolate (UH1.2 isolate) was concluded to have a low level of pathogenesis (non-pathogenesis), which is one of the initial characteristics and characteristics of bioremediation agents. Thus, it can be concluded that there is one indigenous bacterial isolate from the liquid biomedical waste of Hasanuddin University Hospital Makassar that has one of the basic characteristics of a bioremediation agent. However, further tests still need to be carried out to ensure that these isolates are those that have the potential to be used as bioremediation agents, namely hydrolytic enzyme activity tests.
Co-Authors Ahmad Hasyim Anastasia A Basir Anastasia A. Basir Andi Ernawati Ayu Wahyuni Bida Purnamasari, Andi Lohing, Ruben Mudrika Sunarti Mangol, Vivi Wulandhani, Suci Zainuddin