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All Journal Jurnal Kedokteran Brawijaya Medical and Health Journal Jurnal Forensik dan Medikolegal Indonesia
Peramiarti, IDSAP
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Health Professions Medicine & Pharmacology

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Lactic Acid Bacteria (Lactobacillus bulgaricus and Streptococcus thermophillus) in Yogurt Inhibit the Growth of Escherichia coli, Salmonella typhimurium, and Shigella sp. In Vitro Peramiarti, IDSAP
Jurnal Kedokteran Brawijaya Vol 31, No 4 (2021)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2021.031.04.2

Abstract

Diarrhea is defecation with a frequency more often than usual (three times or more) a day (10 mL/kg/day) with a soft or liquid consistency, even in the form of water alone. Pathogenic bacteria, such as Escherichia coli, Salmonella typhimurium, and Shigella sp., play a role in many cases, to which antibiotics are prescribed as the first-line therapy. However, since antibiotic resistance cases are often found, preventive therapies are needed, such as consuming yogurt, which is produced through a fermentation process by lactic acid bacteria (LAB). This research aimed to determine the activity of lactic acid bacteria (Liactobacillus bulgaricus and Streptococcus thermophilus) in yogurt in inhibiting the growth of the pathogenic bacteria E. coli, S. typhimurium, and Shigella sp. The research applied in vitro with the liquid dilution test method and the true experimental design research method with post-test-only and control group design. The design was used to see the inhibitory effect of yogurt LAB on the growth of E. coli, S. typhimurium, and Shigell sp. to compare the effect of several different yogurt concentrations, namely 20%, 40%, 60%, and 80%. The results of the Least Significance Different analysis showed that there was a significant difference between yogurt with a concentration of 0% and that with various concentrations in inhibiting the growth of E. coli, S. typhimurium, and Shigella sp. with a p-value of <0.05. Whereas, there was no significant difference in the various concentrations of yogurt in inhibiting the growth of the three kinds of bacteria with a p-value of > 0.05. 
ROLE OF HYDROGEN PEROXIDE (H2O2) IN YOGHURT (Lactobacillus bulgaricus and Streptococcus thermophilus) IN INHIBITING THE GROWTH OF Staphylococcus epidermidis CAUSES OF ACNE VULGARIS Peramiarti, Idsap; Setiawati, Setiawati; Williem, Williem
Medical and Health Journal Vol 3 No 2 (2024): February
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mhj.2024.3.2.11179

Abstract

Acne vulgaris (AV) is an inflammation of the pilosebaceous follicles caused by the bacterium Staphylococcus epidermidis (S. epidermidis). Yoghurt is the result of fermented milk with a mixture of Lactobacillus bulgaricus and Streptococcus thermophilus as Lactic Acid Bacteria (BAL) which produce several compounds like lactic acid, bacteriocin, hydrogen peroxide, and carbon dioxide. Hydrogen peroxide is effective in reducing inflammatory and non-inflammatory lesions in AV patients. This study aims to determine whether hydrogen peroxide produced by lactic acid bacteria in yoghurt affects inhibiting the growth of S. epidermidis. This research is an experimental study with the post-test only method with control group design which was conducted in vitro. Analysis of H2O2 levels in yoghurt using permanganometric titration. Bacterial growth inhibition test using macro delusion and colony counter. The samples used were 30 samples with two repetitions and divided into 10 concentration groups (0%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%) with 0 % as negative control. Differences in treatment group barriers were analyzed using the One Way ANOVA test. One Way Anova test showed p < 0.001 which indicated a significant difference between each concentration group. The results of the Post-Hoc LSD test were p<0.05 between the control group and all treatment groups. H2O2 in yoghurt has antibacterial activity against S. epidermidis. This study showed that there was an inhibition of the growth of S. epidermidis by yoghurt at concentration of 5% had a hydrogen peroxide content of 0.17 mg/mL and pH of 5.7 was the minimum inhibitory concentration of S. epidermidis growth.
PERBANDINGAN KLORAMFENIKOL, ERITROMISIN DAN FORMALIN DENGAN BERBAGAI DOSIS TERHADAP PENGHAMBATAN PERTUMBUHAN Clostridium perfringens Peramiarti, IDSAP; Syamsu Hidayat, Muhammad Zaenuri
Medical and Health Journal Vol 4 No 1 (2024): August
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mhj.2024.4.1.12735

Abstract

Death is the cessation of function of the circulatory system and respiratory system, or brain stem death has occurred. Decomposition is natural process that occur shortly after death. The main bacteria involved in the decomposition process is Clostridium perfringens. The process of decomposition sometimes need to be inhibited by the preservation bodies. Preserving the corpse is a medical procedure for the provision of certain chemicals such as formalin or can used a broad spectrum antibiotic chloramphenicol and erytromicine. This study aims to test the effectiveness of various doses of chloramphenicol, erytromicine and formaline in inhibiting the growth of Clostridium perfringens. This was an experimental study with post test only and contol group design. The treatment of various doses of chloramphenicol 0,25 µg/ml, 0,5 µg/ml, 1 µg/ml, 2 µg/ml, and 4 µg/ml; Erythromycin doses were 0,2 µg/mL, 0,4 µg/mL, 0,8 µg/mL, 1,6 µg/mL, and 3,2 µg/mL and formalin was given at 2%, 4%, 6%, 8%, and 10% in liquid medium Nutrient Broth. Incubated for 24 hours at 37 ° C, and then planted in agar plate wiith spread plate method to determine the growth of bacteria. Bacterial growth was calculated by colony counter, then the percentage inhibition was determined. Data were analyzed using parametric tests One Way Annova and Post Hoc Test. The effective concentration was determined by Minimum Inhibitory Concentration 50 (MIC50). Data were analyzed using One Way Annova test, showed p value=0,009 (p<0,05) which meant that there were significant difference between the number of colony inhibition with number of doses given to C. perfringens, and the results of Post Hoc Test concentration of formaldehyde 10% and 8% had the lowest number of colonies or has the ability to inhibit the growth of c. perfringens bacteria most of 80% and 63%, so it can be said to be effective concentration (MIC50) at 8% formalin treatment with inhibition of growth of 63%.
The DESCRIPTION OF DIATOMES EXAMINATION IN HEPARIAL ORGANS IN DROWNING CASE IN THE PELUS RIVER SOKARAJA BANYUMAS USING THE WHITE RATS (Rattus novergicus) Imaniah, Ira; Hestiyani, Rani Afifah Nur; Hidayat, Muhammad Zaenuri Syamsu; Peramiarti, IDSAP
Jurnal Forensik dan Medikolegal Indonesia Vol 4 No 1 (2023): Jurnal Forensik dan Medikolegal Indonesia
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jfmi.2023.4.1.7438

Abstract

Diatoms can be used to identificate and diagnose drowning cases because it can enter the body during inhaling in the water. Previous studies have shown that diatoms can be found in the liver of drowning cases, but this cannot be used as evidence of drowning cases. This study aimsto determine diatoms in the liver organs of white rats that drowned in Pelus River. This studyuses an experimental method, with 15 white rats (Rattus norvegicus) which were submerged in water from the Pelus River. Identification of diatoms in the liver was carried out using the acid destruction method. The results of the research are 9 genus of diatoms that can be identified; Amphora, Eunotia, Amphorotia, Simonsenia, Epithemia, Synedra, Ulnaria, Fragilaria, Asterionella. Meanwhile, there were only 5 genus of diatoms identified in the river water samples; Amphora, Eunotia, Craticula, Diadesmis, and Fragilaria. From this research it can be concluded that there are 9 genus of diatoms that can be identified in the livers of white rats that drowned in the fresh waters of the Pelus River
IDENTIFIKASI DIATOMS PADA ORGAN HATI, GINJAL DAN PARU KORBAN TENGGELAM MENGGUNAKAN TIKUS PUTIH (Rattus norvegicus) DI SUNGAI PELUS SOKARAJA PERAMIARTI, IDSAP
Jurnal Forensik dan Medikolegal Indonesia Vol 4 No 2 (2023): Jurnal Forensik dan Medikolegal Indonesia
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jfmi.2023.4.2.9342

Abstract

Tenggelam adalah salah satu penyebab utama kematian ketiga di dunia. Kematian akibat tenggelam yang terjadi di air tawar (sungai, danau, kolam) hampir 90%. Salah satu pemeriksaan khusus untuk mengidentifikasi korban yang meninggal akibat tenggelam adalah dengan pemeriksaan diatom. Diatom adalah fitoplankton uniseluler yang dapat digunakan sebagai bukti intravitalitas korban tenggelam, artinya korban masuk ke dalam air saat masih hidup dan kemudian mati. Penelitian ini bertujuan untuk mengetahui diatom pada organ hepar, ginjal dan paru-paru tikus putih yang tenggelam di Sungai Pelus. Penelitian ini menggunakan metode eksperimen, dengan 15 ekor tikus putih (Rattus norvegicus) yang ditenggelamkan di air Sungai Pelus. Identifikasi diatom pada hepar, ginjal dan paru dilakukan dengan metode destruksi asam. Hasil yang diperoleh di hati teridentifikasi 19 diatom dari 7 genera : Amphora, Eunotia, Simonsenia, Epithemia, Fragilaria, Ulnaria, dan Synedra; organ ginjal mengidentifikasi 22 diatom dari 7 genera : Amphora, Navicula, Cymbella, Cocconeis, Epithemia, Fragilaria, dan Ulnaria; dan organ paru-paru teridentifikasi sebanyak 94 diatom dari 10 genera : Amphora, Diadesmis, Navicula, Simonsenia, Nitzschia, Epithemia, Isthmia, Ulnaria, Synedra, dan Aulacoseira. Sedangkan dari hasil pemeriksaan sampel air media Sungai Pelus didapatkan 14 genus diatom yaitu Amphora, Eunotia, Diadesmis, Navicula, Simonsenia, Nitzschia, Cymbella, Cocconeis, Epithemia, Isthmia, Fragilaria, Ulnaria, Synedra, dan Aulacoseira. Dari penelitian ini dapat disimpulkan bahwa genus diatom yang ditemukan di hati, ginjal dan paru-paru sama dengan genus yang ditemukan pada sampel air medium Sungai Pelus.
THE ROLE OF HYDROGEN PEROXIDE (H2O2) IN YOGURT (Lactobacillus bulgaricus and Streptococcus thermophilus) ON THE GROWTH OF Staphylococcus aureus ATCC 29213 Peramiarti, IDSAP; Zain, Najmi
Medical and Health Journal Vol 4 No 2 (2025): February
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mhj.2025.4.2.14865

Abstract

ABSTRAK Yoghurt merupakan produk pangan susu fermentasi dengan bantuan bakteri asam laktat (BAL). BAL merupakan bakteri yang dapat menghasilkan metabolit asam laktat, bakteriosin dan hidrogen peroksida. Beberapa penelitian menyampaikan BAL dalam yoghurt memiliki kemampuan menghambat pertumbuhan bakteri patogen. S. aureus ATCC 29213 adalah Gram positif, coccus yang tersusun tidak beraturan berkelompok seperti buah anggur. S. aureus ATCC 29213 adalah bakteri patogen utama manusia. Penelitian ini bertujuan untuk mengetahui peranan hidrogen peroksida pada yoghurt (L. bulgaricus dan S. thermophilus) terhadap pertumbuhan S. aureus ATCC 29213. Penelitian ini merupakan penelitian eksperimental dengan post-test only with control group design. Jumlah sampel yang digunakan sebanyak 60 sampel dengan dua kali pengulangan dan terbagi dalam 10 kelompok konsentrasi (0%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%) dengan 0% sebagai kontrol negative. Analisis penelitian ini menggunakan Kruskal Wallis (p=0,003). Hasil ini menunjukkan bahwa terdapat perbedaan signifikan antar kelompok. Kemudian dilanjutkan uji Mann Whitney untuk mengetahui perbandingan masing-masing kelompok. Penelitian ini dapat disimpulkan terdapat penghambatan pertumbuhan S. aureus ATCC 29213 oleh yoghurt dengan konsentrasi 5%, kandungan hidrogen peroksida 0,17 mmol/L, pH 5,7 sebagai konsentrasi yoghurt terendah. Kata Kunci: Hidrogen Peroksida, Staphylococcus aureus, Yoghurt
Co-Authors Hidayat, Muhammad Zaenuri Syamsu Imaniah, Ira Rani Afifah Nur Histiyani Setiawati Setiawati Syamsu Hidayat, Muhammad Zaenuri Williem, Williem Zain, Najmi