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All Journal Journal of Food and Pharmaceutical Science Wahana-Bio: Jurnal Biologi dan Pembelajarannya BIOEDUSCIENCE JRST (Jurnal Riset Sains dan Teknologi) Indonesian Food Science and TechnologyJournal FoodTech: Jurnal Teknologi Pangan Journal of halal product and research (JHPR) Keluwih: Jurnal Sains dan Teknologi BiosciED: Journal of Biological Science and Education Journal of Experimental and Clinical Pharmacy (JECP) Muhammadiyah Journal of Nutrition and Food Science (MJNF) Food Scientia: Journal of Food Science and Technology Jurnal Farmasi Sains dan Praktis Asia Pacific Journal of Sustainable Agriculture, Food and Energy (APJSAFE) Eruditio : Indonesia Journal of Food and Drug Safety Journal of Food and Pharmaceutical Sciences Borneo Journal of Pharmacy Kupang Journal of Food and Nutrition Research
Sophian, Alfi
Pusat Pengembangan Pengujian Obat Dan Makanan Nasional, Badan Pengawas Obat Dan Makanan. Jl. Percetakan Negara, No. 23. Jakarta Pusat,10560, Indonesia
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Journal : Indonesian Food Science and TechnologyJournal

 1 
Species DNA Detection Using PGR Gene Genetic Markers in Chicken Nuggets: Species DNA Detection Using PGR Gene Genetic Markers in Chicken Nuggets Sophian, Alfi
Indonesian Food Science and Technology Journal Vol. 5 No. 1 (2021): Volume 5 Nomor 1, December 2021 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v5i1.14618

Abstract

Species DNA detection using genetic markers of the PGR gene on chicken nuggets was carried out to see whether these genetic markers could be used to test species DNA detection in chicken nuggets food products. The test method used in this study is a real-time PCR test method using the SYBR green technique where the test results can be either Ct or Tm values ​​which indicate amplification or detection. The results of DNA isolation showed that the concentration and purity of the isolated DNA were in the range of 35.10 ng/µL – 36.10ng/µL with an average of 35,488 ng/µL. As for the purity value measured at the A260/A280 wavelength, the results were obtained with a purity range between 2,110 – 2,250 with an average of 2,165. For the results of real-time PCR amplification, the Ct value of the chest sample was at 24.50, the Ct LOD value was at 21.20 and the Ct value of the positive control was 27.10. For the Tm value of the busty sample at 81.10, the Tm LOD value at 81.20 and the positive control Tm value at 82.10. In a conclusion, in this study, genetic markers of the PGR gene can be used to test specific DNA detection of chicken species in chicken nugget products.
Detection of Lysteria monocytogenes in Frozen Meatballs Using Real-Time PCR Sophian, Alfi; Purwaningsih, Ratna
Indonesian Food Science and Technology Journal Vol. 6 No. 1 (2022): Volume 6. Number 1, December 2022 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v6i1.20391

Abstract

Detection of pathogenic bacteria Listeria monocytogenes in processed food products of frozen meatballs is one of the critical test parameters in product quality testing. The challenge in testing product quality is how to find a reliable and effective test method to generate test results data. With this research, it is hoped that it can provide information or references for similar research. The method used in this research is the enrichment method with a direct isolation technique and amplification using real-time PCR. Data analysis was carried out based on 2 main criteria which included: (1) Ct analysis (Cycle threshold) and (2) melting temperature (Tm) analysis. From the results of the analysis using real-time PCR, the results of the tested samples were detected at Ct 11.60 with a Tm value of 81.50. For NTC control not detected, this indicates that the master mix used is in good condition and no contamination occurred when testing the sample. The use of this NTC control plays an important role in monitoring the quality of the master mic and test run. For the LOD control and positive control, the results were detected, whereas in the LOD control, the Ct value was detected at 10.08 and the Tm value was at 81.30. for positive control, the value of Ct was detected at 10.85 and the value of Tm was at 82.0. Based on the results of the study, it can be concluded that the test method used can detect Listeria monocytogenes in processed meat products in the form of frozen meatballs.
Co-Authors Abdul Rohman Aditya Anugerah Marusaha Sitorus Andi Syukur Arif Wahyudi Dewi Rahmawati Diana Tri Wulan Eka Putri Juniarti Igirisa Eni Cahyaningsih La Ode Nasir Marcella Sutanta Muhammad Luthfi Amirullah Muhammad Tri Sutrisno Muindar Muindar Muindar, Muindar Nawwaruddin, Hazza Hammam Purwadi Purwadi Ratna Purwaningsih Ratna Purwaningsih Ratna Purwaningsih Rumiyati, Rumiyati Sari, Miftiana Nugraha Sofia Dyah Utami Sri Utaminingsih Sri Utaminingsih Sri Utaminingsih Sri Utaminingsih Tirta Setya Bhakti Utami, Sofia Dyah Yenita . Yusrina Nabila Yustina Yustina