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All Journal Indonesian Journal of Pharmaceutical Science and Technology Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
NANDYAWATI, DEWI
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Environmental Science Immunology & microbiology Medicine & Pharmacology

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Journal : Indonesian Journal of Pharmaceutical Science and Technology

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Immobilization of Penicillin-G Acylase from Bacillus thuringiensis BD1 for Enhanced Amoxicillin Production Using Na-Alginate Entrapment Dewi, Rizky Aulia Prasasti; Widyasti, Erma Widyasti; Dianursanti, Dianursanti; Sriherwanto, Catur; Kusumaningrum, Susi; Rahayu, Maya D.; Putra, Noorendra L.; Hasanah, Nuur F.; Sativa, Rizka G.; Setyahadi, Siswa; Nandyawati, Dewi
Indonesian Journal of Pharmaceutical Science and Technology Vol 12, No 3 (2025)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v12i3.55501

Abstract

Efficient enzymatic production, particularly using Penicillin-G Acylase (PGA), is crucial for synthesizing amoxicillin, a penicillin-type antibiotic. This study optimized PGA immobilization from Bacillus thuringiensis BD1 using Na-alginate to enhance stability and cost-effectiveness. Various Na-alginate concentrations (1%, 1.25%, 1.5%) were tested, with stability assessments at pH 6-9 and temperatures of 30-60 °C, alongside reusability, morphology, and amoxicillin synthesis evaluations. Initial activity was 46.59 U/mg, with optimal immobilization at 1.5% Na-alginate achieving 41.01 U/mg. After four uses, immobilized PGA BD1 retained ±20% activity with optimal conditions at pH 7 and 40 °C. Enhanced amoxicillin synthesis compared to free enzymes highlights its industrial potential. This research demonstrates the feasibility of using immobilized PGA BD1 for scaling up amoxicillin production, offering significant economic and technological benefits.
Co-Authors Catur Sriherwanto, Catur Dewi, Rizky Aulia Prasasti Dianursanti Gebrina, Amanda Dwi Hasanah, Nuur F. Kharis Yohan Abidin, Kharis Yohan Kusumasmarawati, Ambar Dwi Pradiva, Molina Indah Purwoto, Heri Putra, Noorendra L. Putri, Renny Primasari Gustia Rahayu, Maya D. Royanti, Ida Sativa, Rizka G. Siswa Setyahadi Susi Kusumaningrum Widyasti, Erma Widyasti, Erma Widyasti