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All Journal JURNAL SAINS DAN TEKNOLOGI INDONESIA Media Penelitian dan Pengembangan Kesehatan Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Pharmaceutical Science and Technology Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Susi Kusumaningrum
Center of Pharmaceutical and Medical Technology Agency on Assessment and Application of Technology (BPPT)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Education Immunology & microbiology Medicine & Pharmacology Public Health

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PENGUJIAN AKTIVITAS ANTIACNE NANOPARTIKEL KITOSAN – EKSTRAK KULIT BUAH MANGGIS (Garcinia mangostana) Rismana, Eriawan; Kusumaningrum, Susi; Bunga, Olivia; Nizar, Nizar; Marhamah, Marhamah
Media Penelitian dan Pengembangan Kesehatan Vol 24, No 1 Mar (2014)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.469 KB)

Abstract

AbstrakUji aktivitas antiacne dari pasta dan bubuk nanopartikel kitosan – ekstrak kulit manggis telah diamati terhadap daya hambat pertumbuhan Propionibacterium acnes. Bahan pasta nanopartikel kitosan – ekstrak manggis dibuat melalui reaksi gelasi ionik dengan cara mencampurkan larutan kitosan 0,2 % (dalam asam asetat) dengan ekstrak etanol 70 %kulit buah manggis yang kemudian direaksikan dengan natrium tripolifosfat 0,1 %. Sedangkan bahan bubuk nanopartikel kitosan – ekstrak manggis diproduksi melalui metode freeze drying dari bahan pasta nanopartikel. Hasil pengujian antiacne dari bahan pasta dan bubuk nanopartikel kitosan – ekstrak manggis menunjukkan bahwa kedua bahan dapat menghambat pertumbuhan Propionibacterium acnes yang baik dengan konsentrasi terendah 0.15 %.Kata kunci : antiacne, pasta, kitosan, nanopartikel, ekstrak etanol 70 % kulit buah manggisAbstractThe antiacne activity of paste and powder of chitosan – Garcinia mangostana extract nanoparticles were observed that it’s against of Propionibacterium acnes. The paste of nanoparticles has been prepared by ionic gelation reaction by mixture 0.2 % chitosan solution in acetic acid with Garcinia mangostana 70 % ethanol extract and it’s continued byreaction process with 0.1 % sodium tripolyphosphate. Meanwhile the powder of nanoparticles was produced by freeze drying process of a paste of the nanoparticles. The results of antiacne activity of paste and powder of nanoparticles were showed that it’s could against Propionibacterium acnes with the lowest concentration about 0.15 %.Keywords : antiacnes, paste, chitosan, nanoparticles, Garcinia mangostana 70 % ethanol extract
QSAR Analysis of Rocaglamide Derivatives Cytotoxic Activities Using LFER Hansch Model Firdayani, .; Kusumaningrum, Susi; Setyo Utomo, Doddy Irawan; Wibowo, Agung Eru; Chaidir, .
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

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Abstract

Rocaglamide derivatives are the compounds which have featuring cyclopenta[b]tetra-hydrobenzofuran skeleton. Until now it includes more than 50 naturally occurring derivatives. They were chosen to be interesting candidates for possible therapeutic agents primarily in the field of cancer chemotherapy due to their cytotoxic activities data against various cancer cells. A quantitative structure activity relationship (QSAR) studies were done to investigate physicochemical properties of molecule which  contribute to their activities. Series of rocaglamide derivatives have been used and analyzed using linear free energy regression Hansch model for their cytotoxic activities against MONO-MAC-6 leukemia cells, RAJI lymphoma cells and MEL-JUSO melanoma cells. Results showed that the best QSAR equations were revealed involving C Log P and CMR parameters with nonlinear regression relationships in cytotoxic activities of rocaglamide derivatives against cancer cells above.
POTENSI SENYAWA BIOAKTIF TANAMAN GENUS Phyllanthus SEBAGAI INHIBITOR REPLIKASI VIRUS HEPATITIS B Firdayani, .; Kusumaningrum, Susi; Miranti, Yosephine Ria
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 4, No 2 (2017): December 2017
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1040.446 KB) | DOI: 10.29122/jbbi.v4i2.2589

Abstract

Potency of Plant Bioactive Compounds from the Genus Phyllanthus as Hepatitis B Virus Replication InhibitorIn this research, simulations of molecular docking of Phyllanthus bioactive compounds were performed into the core protein of HBV. This simulation aimed to predict the interaction between compounds with virus core protein causing disruption of capsid formation and inhibiting its replication. The docking simulation was completed by Molegro Virtual Docker 6.0. The 3D stable conformation of molecule structures were docked into HBV core protein downloaded from Protein Data Bank, then the results were analyzed to view the minimum energy and interactions that occurred. The coordinate docking was done at the same coordinate as the previously docked reference ligand position and was validated. From the results it was known that repandusinic acid formed the most stable affinity bond with amino acid residues of viral core proteins. Interaction of B chain forming hydrogen bonds with the amino acid residues of Thr 33, Trp 102, Phe 23, Leu 140, Tyr 118 and Ser 141, and C chain with Thr 128, Val 124 and Glu 117.These compounds can be used as marker for anti HBV.Keyword: Bioactive compounds, core protein, HBV , molecular docking, Phyllanthus ABSTRAKPada penelitian ini dilakukan simulasi penambatan molekul senyawa-senyawa bioaktif Phyllanthus ke dalam protein inti virus hepatitis B. Simulasi ini bertujuan untuk memprediksi interaksi terbentuk antara senyawa dengan protein yang menyebabkan terganggunya pembentukan kapsid virus dan menghambat replikasinya. Simulasi penambatan molekul dilakukan menggunakan program Molegro Virtual Docker 6.0. Sebagai reseptor target digunakan struktur 3D protein inti yang diunduh dari Protein Data Bank. Posisi penambatan dilakukan pada koordinat yang sama dengan posisi ligan referensi yang sudah tertambat sebelumnya dan tervalidasi. Dari hasil simulasi diketahui bahwa asam repandusinat membentuk komplek dengan energi afinitas ikatan yang paling kecil dengan residu asam amino protein inti virus. Interaksi terjadi dengan rantai B yang membentuk ikatan hidrogen dengan asam amino Thr 33, Trp 102, Phe 23, Leu 140, Tyr 118 dan Ser 141, dan rantai C dengan asam amino Thr 128, Val 124 dan Glu 117. Senyawa ini dapat dijadikan sebagai marka untuk anti VHB.Kata kunci: Penambatan molekul, Phyllanthus, protein inti, senyawa bioaktif, VHBReceived: 11 December 2017                 Accepted: 27 December 2017           Published: 31 December 2017 
SINTESIS DAN KARAKTERISASI NANOPARTIKEL KITOSAN – EKSTRAK KULIT BUAH MANGGIS (Garcinia Mangostana) rismana, eriawan; Kusumaningrum, susi; p, olivia bunga; rosidah, idah; marhamah, marhamah
Jurnal Sains dan Teknologi Indonesia Vol. 14 No. 3 (2012)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.139 KB) | DOI: 10.29122/jsti.v14i3.925

Abstract

The chitosan – Garcinia Mangostana extract nanoparticles has been prepared by ionic gelation reaction by mixture 0.2 % chitosan solution in acetic acid with Garcinia Mangostana extract and it’s continued by reaction process with 0.1 % sodium tripolyphosphate. The particle size of material was determined by Particle Size Analyzer (PSA) that it showed in the range of 200 – 500 nm. The color, pH, water, α- mangostin, mercury, arsenic, cadmium, lead, totally microbe aerobic, totally mold and yeast, and solvent residue contents of nanoparticles were also examined by many methods that these resulted are yellow, 4.50 – 5.50, 89 – 90 %, 1.05 %, < 0.005 ppm, < 0.01 ppm, < 0.01 ppm, < 0.05 ppm, < 10 CFU/g, < 10 CFU/g and not detected, respectively. The other characterization was also observed that it’sincluded stability andTLC chromatogram. A mixture of nanoparticles with cosmetics bases was showed that it’s increased stability, homogeneity and easy to formed.
POTENSI SENYAWA BIOAKTIF TANAMAN GENUS Phyllanthus SEBAGAI INHIBITOR REPLIKASI VIRUS HEPATITIS B Firdayani, .; Kusumaningrum, Susi; Miranti, Yosephine Ria
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 4 No. 2 (2017): December 2017
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1040.446 KB) | DOI: 10.29122/jbbi.v4i2.2589

Abstract

Potency of Plant Bioactive Compounds from the Genus Phyllanthus as Hepatitis B Virus Replication InhibitorIn this research, simulations of molecular docking of Phyllanthus bioactive compounds were performed into the core protein of HBV. This simulation aimed to predict the interaction between compounds with virus core protein causing disruption of capsid formation and inhibiting its replication. The docking simulation was completed by Molegro Virtual Docker 6.0. The 3D stable conformation of molecule structures were docked into HBV core protein downloaded from Protein Data Bank, then the results were analyzed to view the minimum energy and interactions that occurred. The coordinate docking was done at the same coordinate as the previously docked reference ligand position and was validated. From the results it was known that repandusinic acid formed the most stable affinity bond with amino acid residues of viral core proteins. Interaction of B chain forming hydrogen bonds with the amino acid residues of Thr 33, Trp 102, Phe 23, Leu 140, Tyr 118 and Ser 141, and C chain with Thr 128, Val 124 and Glu 117.These compounds can be used as marker for anti HBV.Keyword: Bioactive compounds, core protein, HBV , molecular docking, Phyllanthus ABSTRAKPada penelitian ini dilakukan simulasi penambatan molekul senyawa-senyawa bioaktif Phyllanthus ke dalam protein inti virus hepatitis B. Simulasi ini bertujuan untuk memprediksi interaksi terbentuk antara senyawa dengan protein yang menyebabkan terganggunya pembentukan kapsid virus dan menghambat replikasinya. Simulasi penambatan molekul dilakukan menggunakan program Molegro Virtual Docker 6.0. Sebagai reseptor target digunakan struktur 3D protein inti yang diunduh dari Protein Data Bank. Posisi penambatan dilakukan pada koordinat yang sama dengan posisi ligan referensi yang sudah tertambat sebelumnya dan tervalidasi. Dari hasil simulasi diketahui bahwa asam repandusinat membentuk komplek dengan energi afinitas ikatan yang paling kecil dengan residu asam amino protein inti virus. Interaksi terjadi dengan rantai B yang membentuk ikatan hidrogen dengan asam amino Thr 33, Trp 102, Phe 23, Leu 140, Tyr 118 dan Ser 141, dan rantai C dengan asam amino Thr 128, Val 124 dan Glu 117. Senyawa ini dapat dijadikan sebagai marka untuk anti VHB.Kata kunci: Penambatan molekul, Phyllanthus, protein inti, senyawa bioaktif, VHBReceived: 11 December 2017                 Accepted: 27 December 2017           Published: 31 December 2017 
DEKSTROSA MONOHIDRAT KUALITAS FARMASI DARI PATI Manihot ecsulenta, Metroxylon sagu, Zea mays, Oryza sativa, dan Triticum Kartika, Bayu Mahdi; Khojayanti, Lely; Nuha, .; Listiana, Shelvi; Kusumaningrum, Susi; Wijaya, Ayustiyan Futu
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1230.738 KB) | DOI: 10.29122/jbbi.v6i2.3208

Abstract

Pharmaceutical Grade Dextrose Monohydrate from Manihot ecsulenta, Metroxylon sagu, Zea mays, Oryza sativa, dan Triticum Starch ABSTRACT Pharmaceutical-grade dextrose monohydrate, one of raw materials used as active pharmaceutical ingredients (API) and additives, can be made from starch. There are five types of local Indonesian commercial starch that are potentially used, namely tapioca (Manihot esculenta), sago (Metroxylon sagu), corn (Zea mays), rice (Oryza sativa), and wheat (Triticum) starch. This study aimed to compare these five starches as raw materials for preparing pharmaceutical-grade dextrose monohydrate which was expected to meet the requirements of the Indonesian Pharmacopoeia (5th Edition) and the United States Pharmacopeia (USP). The starch was converted into dextrose monohydrate through liquefaction hydrolysis, saccharification hydrolysis, activated carbon purification and filtration, ion exchange purification, evaporation, crystallization and drying.  High Performance Liquid Chromatogram (HPLC) and the Luff-Schoorl methods were used for dextrose equivalent value (DE) analysis. The results showed that only three of the starch types produced pharmaceutical-grade dextrose monohydrate, namely (DE) sago starch (107.23% and 100.77%), corn starch (97.86% and 96.19%), and tapioca starch (85.18% and 99.20%).Keywords: dextrose equivalent, dextrose monohydrate, hydrolysis, pharmaceutical grade, starchABSTRAKDekstrosa monohidrat kualitas farmasi, salah satu bahan baku yang digunakan sebagai active pharmaceutical ingredient (API) dan bahan tambahan, dapat dibuat dari bahan pati-patian. Terdapat lima jenis pati komersial lokal Indonesia yang berpotensi digunakan yakni pati tapioka (Manihot esculenta), pati sagu (Metroxylon sagu), pati jagung (Zea mays), pati beras (Oryza sativa), dan pati gandum (Triticum). Penelitian ini bertujuan membandingkan lima jenis pati tersebut sebagai bahan baku pembuatan dekstrosa monohidrat kualitas farmasi yang diharapkan mampu memenuhi standar persyaratan dari Farmakope Indonesia Edisi V dan United States Pharmacopeia (USP). Pati diubah menjadi dekstrosa monohidrat melalui hidrolisis likuifikasi, hidrolisis sakarifikasi, pemurnian karbon aktif dan filtrasi, pemurnian ion exchange, evaporasi, kristalisasi dan pengeringan. Metode High Performance Liquid Chromatogram (HPLC) dan Luff-Schoorl digunakan untuk analisis dextrose equivalent (DE). Hasil penelitian menunjukkan hanya tiga jenis pati yang menghasilkan dekstrosa monohidrat kualitas farmasi, yakni (DE) pati sagu (107,23% dan 100,77%), pati jagung (97,86% dan 96,19%), dan pati tapioka (85,18% dan 99,20%).Kata kunci: dekstrosa monohidrat, dextrose ekuivalen, hidrolisis, kualitas farmasi, pati
Immobilization of Penicillin-G Acylase from Bacillus thuringiensis BD1 for Enhanced Amoxicillin Production Using Na-Alginate Entrapment Dewi, Rizky Aulia Prasasti; Widyasti, Erma Widyasti; Dianursanti, Dianursanti; Sriherwanto, Catur; Kusumaningrum, Susi; Rahayu, Maya D.; Putra, Noorendra L.; Hasanah, Nuur F.; Sativa, Rizka G.; Setyahadi, Siswa; Nandyawati, Dewi
Indonesian Journal of Pharmaceutical Science and Technology Vol 12, No 3 (2025)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v12i3.55501

Abstract

Efficient enzymatic production, particularly using Penicillin-G Acylase (PGA), is crucial for synthesizing amoxicillin, a penicillin-type antibiotic. This study optimized PGA immobilization from Bacillus thuringiensis BD1 using Na-alginate to enhance stability and cost-effectiveness. Various Na-alginate concentrations (1%, 1.25%, 1.5%) were tested, with stability assessments at pH 6-9 and temperatures of 30-60 °C, alongside reusability, morphology, and amoxicillin synthesis evaluations. Initial activity was 46.59 U/mg, with optimal immobilization at 1.5% Na-alginate achieving 41.01 U/mg. After four uses, immobilized PGA BD1 retained ±20% activity with optimal conditions at pH 7 and 40 °C. Enhanced amoxicillin synthesis compared to free enzymes highlights its industrial potential. This research demonstrates the feasibility of using immobilized PGA BD1 for scaling up amoxicillin production, offering significant economic and technological benefits.
Co-Authors . Chaidir . Firdayani Agung Eru Wibowo Catur Sriherwanto, Catur Dewi, Rizky Aulia Prasasti Dianursanti Doddy Irawan Setyo Utomo Eriawan Rismana Hasanah, Nuur F. Idah Rosidah Kartika, Bayu Mahdi Khojayanti, Lely Listiana, Shelvi Marhamah Marhamah Miranti, Yosephine Ria Miranti, Yosephine Ria NANDYAWATI, DEWI Nizar Nizar Nuha, . Olivia Bunga p, olivia bunga Putra, Noorendra L. Rahayu, Maya D. Sativa, Rizka G. Siswa Setyahadi Widyasti, Erma Widyasti Wijaya, Ayustiyan Futu