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Dianursanti
Departemen Teknik Kimia Universitas Indonesia, Kampus Baru UI Depok Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Electrical & Electronics Engineering Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Other

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BIOADSORPSI SPESI SULFUR DALAM ION TIOSULFAT OLEH THIOBACILLUS THIOPARUS DENGAN BANTUAN ZEOLIT ALAM LAMPUNG SEBAGAI MEDIUM ADSORPSI Hidayanto, Ariyo Prabowo; Dianursanti, Dianursanti; Karamah, Eva F; Wulan, Praswasti PDK
Indonesian Journal of Biotechnology and Biodiversity Vol 1, No 1 (2017): Indonesian Journal of Biotechnology and Biodiversity
Publisher : Universitas Esa Unggul

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Abstract

AbstrakSelain dapat menurunkan keekonomisan gas bumi, gas buang yang mengandung senyawa sulfur juga memiliki potensi yang dapat menimbulkan pencemaran udara jika terlepas ke udara. Untuk mengurangi kadar senyawa ini, proses bioadsorpsi dapat digunakan dengan menggabungkan proses degradasi oleh aktivitas mikroba dalam hal ini melalui aktivitas bakteri Thiobacillus thioparus dengan proses adsorpsi melalui bantuan material berpori yaitu zeolit alam Lampung sebagai adsorben. Pada tahap pembuatan medium dan fase tumbuh kultur Thiobacillus thioparus, koloni sel dijaga pada fase log yang akan terbentuk mulai hari ke 4 dan berhenti pada hari ke 8 dengan pH optimum harus stabil pada rentang 6 – 8. Pada fase inilah kultur digunakan pada proses bioadsorpsi. Zeolit sebagai bahan pengisi kolom bioadsorpsi juga sebelumnya di preparasi dengan proses mekanik untuk menyeragamkan ukuran diameter partikel yaitu sebesar ± 1 – 2 mm. BET autosorb digunakan untuk mengetahui profil fisik dari zeolit yaitu luas permukaan, volume pori, dan diameter pori. Pada proses bioadsorpsi, persentase reduksi tertinggi spesi sulfur pada ion tiosulfat dicapai sebesar 41,31 % pada jam ke 8. Sedangkan, proses bioadsorpsi akan terus berlanjut sampai mencapai jam ke 24 hingga tak tersedianya lagi sulfur yang dapat didegradasi oleh Thiobacillus thioparus, maka sel memasuki fase kematian hingga mengakhiri proses yang terjadi.Kata kunci : Thiobacillus thioparus, zeolit alam lampung, bioadsorpsi
Peningkatan produksi biomassa Chlorella vuldaris melalui perlakuan teknik pemerangkapan sel dalam aliran sirkulasi media kultur D Dianursanti; Rachma Nuzulliany; Anondho Wijanarko; M Nasikin
Jurnal Teknik Kimia Indonesia Vol 8, No 3 (2009)
Publisher : ASOSIASI PENDIDIKAN TINGGI TEKNIK KIMIA INDONESIA (APTEKIM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/jtki.2009.8.3.3

Abstract

Recently, Chlorella vulgaris is widely studied by experts for its ability as a food supplement and health.  Cultivation of Chlorella vulgaris can be used as a tool to reduce global warming. Chlorella vulgaris can efficiently reduce CO2 because they can grow quickly and easily adapted into the photobioreactor system engineering. This research uses continuous illumination of 5000 lux. However, this lighting condition has limitations because of the intensity given is always constant while the number of Chlorella vulgaris in culture increased. Therefore, one solution to solve it is by using the entrapment of cell or filtration process. Chlorella vulgaris cultivation process carried out in bubble column photobioreactor medium flowed by air containing 5% CO2 with a flow rate of 15.66 m / hr. The process of Chlorella vulgaris cultivation with filtration technique has successfully increased production of biomass up to 1.03 times compared with continuous lighting without filtration with the same amount of inoculum. The final result of dry weight biomass obtained was 0.00756 g/dm3 with shorter cultivation period, 200 hours.  Keywords: filtration, Chlorella vulgaris., constant lighting intensity, bubble column photobioreactor, CO2 fixation.AbstrakBelakangan ini, Chlorella vulgaris diminati oleh para ahli untuk diteliti karena kemampuannya sebagai penghasil biomassa yang bermanfaat sebagai suplemen makanan dan kesehatan. Dalam proses pembudidayaannya, Chlorella vulgaris ini dapat dimanfaatkan sebagai pereduksi pemanasan global. Chlorella vulgaris dapat dengan efisien mereduksi CO2 karena mereka dapat tumbuh dengan cepat dan mudah diadaptasikan ke dalam rekayasa sistem fotobioreaktor. Pencahayaan yang diberlakukan pada penelitian ini adalah pencahayaan kontinu dengan intensitas 5000 lux. Namun, pencahayaan ini memiliki keterbatasan karena intensitas yang diberikan selalu konstan padahal jumlah Chlorella vulgaris dalam kultur semakin meningkat. Oleh sebab itu, salah satu upaya yang dapat dilakukan untuk mengatasinya adalah dengan menggunakan proses pemerangkapan sel atau  filtrasi. Proses Kultivasi Chlorella vulgaris dilakukan dalam fotobioreaktor kolom gelembung skala menengah yang dialiri oleh udara yang mengandung 5% CO2 dengan laju alir sebesar 15,66 m/jam. Proses filtrasi untuk kultivasi Chlorella vulgaris berhasil meningkatkan produksi biomassanya hingga 1,03 kali lipat dibandingkan dengan pencahayaan kontinu tanpa filtrasi dengan jumlah inokulum yang sama. Hasil akhir produksi biomassa adalah 0,00756 g/dm3 dengan masa kultivasi yang lebih singkat yaitu selama 200 jam.Keywords: filtrasi, Chlorella vulgaris., pencahayaan kontinu, fotobioreaktor kolom gelembung, fiksasi CO2.
PENGARUH PENGURANGAN AIR TERPRODUKSI TERHADAP FLOW REGIME PADA PIPA PENYALUR DI LAPANGAN PHE-30 PT PHE WMO Dibran Paramartha; Dianursanti Dianursanti
Prosiding Seminar Nasional Pakar Prosiding Seminar Nasional Pakar 2019 Buku I
Publisher : Lembaga Penelitian Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/pakar.v0i0.4182

Abstract

PT Pertamina Hulu Energi West Madura Offshore (PHE WMO) berupayameningkatkan produksi minyak, salah satu cara yaitu melakukan optimalisasi pipa penyalur dari PHE-30 menuju fasilitas proses PPP dengan mengurangi slug flow pada pipa penyalur tersebut. Saat ini produksi minyak, gas dan air dari lapangan PHE-30 sebesar 2.070 Bopd, 5,1 MMscfd dan 10.800 Bwpd. Produksi minyak dari lapangan PHE-30 dapat dioptimalkan dengan menjaga gas liquid ratio (GLR) pada pipa penyalur tersebut. Optimalisasi ditujukan untuk menjaga fasilitas proses tetap aman dan terhidar dari kondisi shutdown atau terhenti sementara akibat terjadinya slug flow yang terjadi disepanjang pipa penyalur.Slug flow terjadi karena ketidakseimbangan perbandingan antara distribusi laju alir gas dan laju alir liquid pada pipa penyalur atau dapat disebut dengan gas liquid ratio (GLR).Perbedaan GLR ini disebabkan oleh tingginya air terproduksi dari dalam sumur PHE-30. Analisa dilakukan dengan menggunakan software Pipesim untuk melakukan simulasi flow regime pada pipa penyalur dari PHE-30 menuju PPP. Parameter yang digunakan yaitu laju alir minyak, gas dan air serta kecepatan superficial gas dan liquid. Hasil menunjukkan bahwa dengan pengurangan air terproduksi dari lapangan PHE-30 dengan GLR 2.463 scf/bbl, dapat merubah kondisi flow regime dari yang sebelumnya slug flow menjadi stratified flow.
NOx Enriched Flue Gas Fixation for Biomass Production of Chlorella Vulgaris Buitenzorg Dianursanti Dianursanti; Mohammad Nasikin; Anondho Wijanarko
ASEAN Journal of Chemical Engineering Vol 10, No 1 (2010)
Publisher : Department of Chemical Engineering, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.188 KB) | DOI: 10.22146/ajche.50091

Abstract

Cultivation of Chlorella vulgaris Buitenzorg in a pilot scale of bubble column photo bioreactor using simulated NOx enriched flue gas concluded that presence of N2O as simulated NOx pollution (0.02%) in blowing bubbled air and CO2 is not so significant, compare to control experiment that was designed by absence of N2O (around 20% decreased). Meanwhile, presence of N2O tends a less significantly decreasing of μ - specific growth rate and qCO2 – specific CO2 transferred rate. It is around 30% decreased in both of μ and qCO2. Then, cultivation by presence of NOx in blowing simulated flue gas could drastically decreased intracellular carotene and lipid content and become increase to level near to both of pigment and lipid content in control experiment. Furthermore, cultivation by presence of NOx in blowing simulated flue gas also could drastically exchange intracellular fatty acid content and it become dominated by 16:0 species. Finally, refreshing cellular growth product with re-cultivation by blowing fresh air, could be restored the fatty acid content nearly to beginning microbial fatty acid content. It was happened cause of converting hexadecanoate species to octadecanoate species and it was shown that oleate (18:1) was dominating species.
Enhanced Chlorella vulgaris Buitenzorg growth by Photon Flux Density Alteration in Serial Bubble Column Photobioreactors Anondho Wijanarko; Dianursanti Antonius Yudi Sendjaya; Misri Gozan; Roekmijati Widaningroem Soemantojo; Arief Budi Witarto; Kazuhiro Asami Kazuhisa Ohtaguchi
ASEAN Journal of Chemical Engineering Vol 7, No 1 & 2 (2007)
Publisher : Department of Chemical Engineering, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.265 KB) | DOI: 10.22146/ajche.50131

Abstract

Micro algae are photolitotrophs that perform oxygenic photosynthesis and capable of accumulating a large amount of CO2, using an inducible CO2 concentrating mechanism (CCM). These characteristics make the micro algae potentially useful for removal and utilization of CO2 emitted from industrial plant. Generally, the usage of photosynthetic microorganism in CO2 fixation and biomass production for the economically viable commodities have been increased and significantly improved as a solution for this problem. Using these facts and previous research results using Anabaena cylindrica IAM M1 and Spirulina platensis IAM M 135, enhancement of CO2 fixation and biomass production by Chlorella vulgaris Buitenzorg with photon flux density alteration along with an increasing of culture biomass during the cellular growth period, was implemented in this research. The photon flux density used in this alteration was the maximum light for Chlorella’s maximum growth rate ( I mmax,opt ). The cultivation of Chlorella vulgaris Buitenzorg in the Benneck basal medium operating conditions: T, 29oC; P, 1.0 atm; UG, 2.4m/h; CO2, 10%; using Philip Halogen Lamp 20W/12V/50Hz as the light source and three bubble column photobioreactors arranged in series order with each having a volume of 0.200dm3. Results had shown that the photon flux density alteration as a whole could increase around 60% the biomass production of Chlorella vulgaris and around 7% the CO2 fixation ability, compared to constant photon flux density outcomes. This experiment also showed that the noncompetitive inhibition of [HCO3-] as carbon source substrate is affected significantly during the cultivation in both of alteration and continuous photon flux density.
Effect of Photoperiodicity on Co2 Fixation By Chlorella vulgaris Buitenzorg in Bubble Column Photobioreactor For Food Supplement Production Wijanarko, Anondho; Dianursanti, Dianursanti; Witarto, Arief Budi; Soemantojo, Roekmijati Widaningroem
Makara Journal of Technology Vol. 8, No. 2
Publisher : UI Scholars Hub

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Abstract

To reduce the level of CO2 content in air, effort on converting CO2 to useful products is required. One of the alternatives includes CO2 fixation to produce biomass using Chlorella vulgaris Buitenzorg. Chlorella vulgaris Buitenzorg is applied for production of food supplement. Chlorella vulgaris Buitenzorg is also easy to handle due to its superior adaptation. Currently, Chlorella vulgaris Buitenzorg has been analyzed by some experts for its cellular composition, its ability to produce high quality biomass and the content of essential nutrition. A series of experiments was conducted by culturing Chlorella vulgaris Buitenzorg using Beneck medium in bubbling column photobioreactor. The main variation in this experiment was photoperiodicity, where growth of Chlorella vulgaris Buitenzorg was examined during photoperiodicity condition. The difference between CO2 gas concentration of inlet and outlet of the reactor during operational period, was compared to the same experiment under continuous illumination. Under photoperiodicity of 8 and 9 h/d, the culture cell densities (N) were approximately 40 % higher than under continuous illumination. Final biomass density of Chlorella vulgaris Buitenzorg at 9 h/d illumination was 1.43 g/dm3, around 46% higher than under continuous illumination. Specific carbon dioxide transfer rate (qCO2) in photoperiodicity was 50-80% higher than under continuous illumination. These experiments showed that photoperiodicity affects the growth of Chlorella vulgaris Buitenzorg The specific growth rate (μ) by photoperiodicity was higher than that by continuous ilumination while the growth period was two times longer. Based on the experiments, it can be concluded that photoperiodicity might save light energy consumption. The prediction of kinetic model under continuous illumination as well as under photoperiodicity illumination showed that Haldane model became the fitted kinetic model.
Kinetic Model For Triglyceride Hydrolysis Using Lipase:Review Hermansyah, Heri; Wijanarko, A.; Dianursanti, Dianursanti; Gozan, Misri; Wulan, Praswasti P. D.K; Arbianti, Rita; Soemantojo, Roekmijati W.; Utami, Tania Surya; Yuliusman, Yuliusman; Kubo, Momoji; Kitakawa, Naomi Shibasaki; Yonemoto, Toshiy
Makara Journal of Technology Vol. 11, No. 1
Publisher : UI Scholars Hub

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Abstract

Triglyceride hydrolysis using lipase has been proposed as a novel method to produce raw materials in food and cosmetic industries such as diacylglycerol, monoacylglycerol, glycerol and fatty acid. In order to design a reactor for utilizing this reaction on industrial scale, constructing a kinetic model is important. Since the substrates are oil and water, the hydrolysis takes place at oil-water interface. Furthermore, the triglyceride has three ester bonds, so that the hydrolysis stepwise proceeds. Thus, the reaction mechanism is very complicated. The difference between the interfacial and bulk concentrations of the enzyme, substrates and products, and the interfacial enzymatic reaction mechanism should be considered in the model.
Biomass Production Chlorella Vulgaris Buitenzorg Using Series of Bubble Column Photo Bioreactor with a Periodic Illumination Wijanarko, Anondho; Dianursanti, Dianursanti; Muryanto, Muryanto; Simanjuntak, Josia; Kencana Wulan, Praswasti Pembangun Dyah; Hermansyah, Heri; Gozan, Misri; Soemantojo, Roekmijati Widaningroem
Makara Journal of Technology Vol. 12, No. 1
Publisher : UI Scholars Hub

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Abstract

Chlorella vulgaris Buitenzorg cultivation using three bubble column photo bioreactors arranged in series with a volume of 200 mL for 130 hours shows an increase of biomass production of Chlorella vulgaris Buitenzorg up to 1.20 times and a decrease of the ability of CO2 fixation compared to single reactor at a periodic sun illumination cycle. The operation conditions on cultivation are as following: T, 29.0oC; P,1 atm.; UG, 2.40 m/h; CO2, 10%; Benneck medium; and illumination source by Phillip Halogen Lamp 20W /12V/ 50Hz. Other research parameters such as microbial carbon dioxide transferred rate (qco2), CO2 transferred rate (CTR), energy consumption for cellular formation (Ex), and cultural bicarbonate species concentration [HCO3] also give better results on series of reactor.
Effect of Light Quality, Light Intensity, and Cell Inoculum Arrangement on Growth, Pigment and Carbon Content from Spirulina platensis using LED Light Dianursanti; Chempaka, Rayi Mishellia; Hartono, Nelvina; Habibah, Lulu; Praharyawan, Swastika
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1123-1134

Abstract

This study evaluates the effect of lighting and cell inoculum in Spirulina platensis cultivation on its growth, pigment composition, and ability to capture CO2. Different light intensities (1,000, 3,000, and 5,000 lux), light quality (white, blue, and red), and cell inoculum which are shown as OD values (0.2, 0.3, and 0.5) were assessed. The highest growth rate was obtained from red light, followed by white and blue light. The maximum biomass concentration (0.0711 mg/ml) was obtained when red light was used under 5,000 lux light intensity. The highest carbon content (5.1274 mg/ml algae) was also obtained during red light cultivation under 5,000 lux light intensity. Meanwhile, the highest chlorophyll (1.4365 mg/mg algae) content was obtained at blue light cultivation under 5,000 lux intensity and OD 0.5 cell inoculum. The highest phycocyanin (0.0309 mg/mg algae) was obtained under red light with 3,000 lux light intensity and OD 0.5 cell inoculum. It was found that the cultivation conditions to achieve high biomass and high pigment concentration were different.
Immobilization of Penicillin-G Acylase from Bacillus thuringiensis BD1 for Enhanced Amoxicillin Production Using Na-Alginate Entrapment Dewi, Rizky Aulia Prasasti; Widyasti, Erma Widyasti; Dianursanti, Dianursanti; Sriherwanto, Catur; Kusumaningrum, Susi; Rahayu, Maya D.; Putra, Noorendra L.; Hasanah, Nuur F.; Sativa, Rizka G.; Setyahadi, Siswa; Nandyawati, Dewi
Indonesian Journal of Pharmaceutical Science and Technology Vol 12, No 3 (2025)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v12i3.55501

Abstract

Efficient enzymatic production, particularly using Penicillin-G Acylase (PGA), is crucial for synthesizing amoxicillin, a penicillin-type antibiotic. This study optimized PGA immobilization from Bacillus thuringiensis BD1 using Na-alginate to enhance stability and cost-effectiveness. Various Na-alginate concentrations (1%, 1.25%, 1.5%) were tested, with stability assessments at pH 6-9 and temperatures of 30-60 °C, alongside reusability, morphology, and amoxicillin synthesis evaluations. Initial activity was 46.59 U/mg, with optimal immobilization at 1.5% Na-alginate achieving 41.01 U/mg. After four uses, immobilized PGA BD1 retained ±20% activity with optimal conditions at pH 7 and 40 °C. Enhanced amoxicillin synthesis compared to free enzymes highlights its industrial potential. This research demonstrates the feasibility of using immobilized PGA BD1 for scaling up amoxicillin production, offering significant economic and technological benefits.
Co-Authors Aida R. Pudjiastuti Albert Santoso Anondho Wijanarko Anondho Wijanarko Anondho Wijanarko Anondho Wijanarko Arief Budi Witarto Arief Witarto Arief Witarto, Arief Catur Sriherwanto, Catur Chempaka, Rayi Mishellia Dewi, Rizky Aulia Prasasti Dibran Paramartha Elsa Krisanti Eny Kusrini Habibah, Lulu Hanung Adi Nugroho Hartono, Nelvina Hasanah, Nuur F. Heri Hermansyah Hidayanto, Ariyo Prabowo Josia Simanjuntak Josia Simanjuntak Kamarza Mulia, Kamarza Karamah, Eva F Kazuhiro Asami Kazuhisa Ohtaguchi Kitakawa, Naomi Shibasaki M Nasikin Maemonah, Maemonah Misri Gozan Mohammad Nasikin Momoji Kubo Momoji Kubo, Momoji Muryanto Muryanto Muryanto Muryanto, Muryanto Muthia Delaamira, Muthia NANDYAWATI, DEWI Naomi Shibasaki-Kitakawa Naomi Shibasaki-Kitakawa, Naomi Ng Sha Shiong Praswasti P. D.K Wulan Praswasti P. D.K Wulan, Praswasti P. D.K Praswasti PDK Wulan, Praswasti PDK Praswasti Pembangun Dyah Kencana Wulan Praswasti Wulan Praswasti Wulan, Praswasti Putra, Noorendra L. Rachma Nuzulliany Rahayu, Maya D. Rita Arbianti Roekmijati Soemantojo Roekmijati Soemantojo Roekmijati Soemantojo, Roekmijati Roekmijati W. Soemantojo Roekmijati W. Soemantojo, Roekmijati W. Roekmijati Widaningroem Soemantojo Sativa, Rizka G. Siswa Setyahadi Soemantojo, Roekmijati Widaningroem Susi Kusumaningrum Swastika Praharyawan, Swastika Syafrizal Syafrizal Tania Surya Utami Tegar Maulana, Tegar Toshiy Yonemoto Toshiy Yonemoto, Toshiy Widyasti, Erma Widyasti Wijanarko, A. Yoki Yulizar Yosmarina Harahap Yuliusman Yuliusman