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All Journal JURNAL SAINS DAN TEKNOLOGI INDONESIA MEDIA INFORMASI Bioscientist : Jurnal Ilmiah Biologi Jurnal Buana Farma Pharmaceutical Sciences and Research (PSR) Sciences of Pharmacy CERATA
Siswa Setyahadi
Badan Pengkajian dan Penerapan Teknologi - BPPT
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences

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ISOLASI DAN IDENTIFIKASI SENYAWA KIMIA DARI EKSTRAK ETIL ASETAT DAUN SIRSAK DAN UJI BAKTERI STREPTOCOCCUS MUTANS ATCC 31987 Herdiana, Irvan; Setyahadi, Siswa; S, Partomuan
Media Informasi Vol 13, No 2 (2017): BULETIN MEDIA INFORMASI
Publisher : Poltekkes Kemenkes Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.428 KB)

Abstract

Berbagai obat antibiotik telah banyak digunakan secara klinis untuk  pengobatan infeksi  yang disebabkan oleh bakteri gram positif maupun gram negatif. Namun obat antibiotik tersebut memiliki sejumlah efek samping, baik ringan maupun berat. Bakteri Streptococcus mutans adalah bakteri penyebab utama dalam pembentukan karies gigi. Bermanfaat sebagai pengobatan infeksi. Tujuan penelitian ini adalah untuk mengetahui senyawa dari fraksi etil asetat daun sirsak yang berperan sebagai antibakteri dalam menghambat pertumbuhan bakteri Streptococcus mutans ATCC 31987. Studi aktivitas penghambatan pertumbuhan bakteri dilakukan secara invitro dengan metode diameter daya hambat dengan mengukur diameter zona bening di sekitar disk pada media padat yang ditumbuhi bakteri menggunakan jangka sorong. Hasil penelitian menunjukkan bahwa fraksi etil asetat daun tumbuhan sirsak dari isolat Sub Fraksi F.7.IV memiliki aktivitas antibakteri melalui daya hambat pertumbuhan bakteri Streptococcus mutans ATCC 31987 pada konsentrasi 40%, 20%, 10% dan 5% dengan kategori tidak aktif, Analisis data dan identifikasi senyawa dilakukan dengan LCMS dan FT-IR, terdapat campuran 3 senyawa yaitu; annopentocin C, annopentocin B dan annomuricin D-one.
DEMINERALISASI DAN DEPROTEINASI KULIT UDANG SECARA KONTINYU PADA TAHAPAN EKSTRAKSI KITIN SECARA BIOLOGIS waltam, deden rosid; Hermansyah, Heri; Setyahadi, siswa
Jurnal Sains dan Teknologi Indonesia Vol. 12 No. 1 (2010)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (232.55 KB) | DOI: 10.29122/jsti.v12i1.843

Abstract

Chitin extraction in industry has been conducted by chemical process. The process has been known as a harsh treatment that badly affected to chitin quality, equipment and the environment. Since the last decade biologically chitin extractionhas more attracted attention. The biologically chitin extraction was conducted by batch fermentation or subsequent-batch fermentation. Continous demineralization and deproteinization is a new inovation on biologically chitin production technology.This system promises as an alternative technology for overcoming problems of batch fermentation process and chemical process. The objectives of the experiment was to obtain the optimal condition for continous deminineralizationand deproteinization for producing chitin from Panaeus vannamei shrimp shells. Lactobacillus acidophilus FNCC 116 and Bacillus licheniformis F11.1 was used for demineralization and deproteination process respectively. The results showed that the best condition for continuous demineralization was 6,5% glucose feed, with 16 hours retention time. For continuous deproteinization, the best condition was with 12 hours retention time. The process could remove 92.95% ash and 91.40% protein. The chitin, ash, and protein content of chitin product was 96.69%, 1.44% and 1,76% respectively.
Amobilisasi Sel Lactobacillus Acidophilus FNCC116 Untuk Demineralisasi Limbah Kulit Udang Dalam Pengolahan Kitin Betha, Ofa Suzanti; Setyahadi, Siswa; Suryadi, Herman
Majalah Ilmu Kefarmasian Vol. 6, No. 3
Publisher : UI Scholars Hub

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Abstract

Chitin, a homopolimer, is the most abundant renewable natural resources after cellulose. Chitin and its derivatives hold many applications in agriculture, textile, pharmacy and medic. Chitin that extracted from waste shrimp shells by biological fermentation has better quality than chemical procees. Demineralization of chitin by biological procees use lactic acid as product of fermentation. Deproteinization of chitin use proteolytic activity of enzyme that produce by bacteria in fermentation. Lacto-bacillus acidophilus FNCC116 has been immobilized by entrapment methods and 2% sodium alginate in 0,2 M CaCl2 as the matric . The ability of immobilized Lacto-bacillus acidophilus FNCC116 cell in fermentation was tested. The fermentation that was carried out in medium which consist of 6% glukosa, 1,5% yeast extract, 0,003% MnSO4 0,003% FeSO4.7H2O, 0,02% MgSO4.7H2O and has been producted 2,24% lactic acid. Demineralization of waste shrimp shell with 30% immobilized Lactobacillus acidophilus FNCC116 cell has successfully decreased ash content tol 1,18% and produced lactic acid maximum 2.24%. Immobilization of Lactobacillus acidophilus FNCC116 cell promised an efficient method in bioproceesing of chitin recovery.
PURIFIKASI DAN KARAKTERISASI ENZIM KOLAGENASE DARI Bacillus sp. KUB BPPT CC DENGAN MENGGUNAKAN SUBSTRAT KOLAGEN DARI KULIT CEKER AYAM Febrina, Melia; Setyahadi, Siswa; Churiyah
CERATA Jurnal Ilmu Farmasi Vol 13 No 1 (2022): Cerata Jurnal Ilmu Farmasi
Publisher : Universitas Muhammadiyah Klaten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61902/cerata.v13i1.454

Abstract

Kolagenase merupakan endopeptidase yang dapat memecah domain triple helix dari kolagen. Sumber utama substrat kolagen yang digunakan pada penelitian ini adalah kulit ceker ayam, untuk memanfaatkan limbah dari Rumah Potong Ayam dengan jumlah cukup banyak. Ceker ayam merupakan salah satu bagian dari tubuh ayam yang kurang diminati, terdiri atas komponen kulit, otot dan tulang dengan kandungan kolagen yang tinggi pada bagian kulit. Karakteristik kolagen dari sumber yang berbeda menghasilkan karakteristik enzim kolagenase yang berbeda pula. Sehingga dibutuhkan informasi mengenai karakteristik enzim dan aktivitas enzim yang optimum. Penelitian ini bertujuan untuk ekstraksi kolagen, penentuan berat molekul kolagen, karakterisasi kolagen, produksi kolagenase, pemurnian enzim kolagenase, karakterisasi enzim kolagenase crude dan murni. Produksi kolagenase dengan menggunakan media Luria Bertani Broth (LB) cair dan menambahkan 5% kulit ceker ayam. Fermentasi dengan Bacillus sp. KUB BPPT CC dengan variasi waktu 24, 36 dan 48 jam. Waktu produksi optimum adalah 36 jam dengan aktivitas kolagenase 0,1001 U/ml. Pemurnian enzim dilakukan dengan presipitasi amonium sulfat mulai dari konsentrasi 0 hingga 80%, dimana hasil aktivitas optimum dengan presipitasi amonium sulfat 60% (b/v), dilanjutkan dengan kromatografi kolom DEAE Sephadex A-50. Pengendapan dengan amonium sulfat mengakibatkan pemurnian 2,43 kali lipat. Setelah pemurnian dengan kolom DEAE Sephadex A-50, enzim dimurnikan 11,21 kali lipat. Berat molekul enzim kolagenase 42 kDa. Aktivitas optimum kolagenase crude dan murni pada suhu 50oC. Enzim crude memiliki pH optimum 10, sedangkan enzim yang dimurnikan pada pH 7. Pada pengujian aktivitas kolagenase digunakan substrat yang berasal dari kulit ceker ayam. Metode ekstraksi kolagen tergolong kedalam kolagen larut asam dengan uji kolagen yaitu Nilai rendemen kolagen 9,475%, Nilai pH 6,8, Kadar air 5%, Kadar Abu 0,6%, Kadar protein 0,425 mg/ml dan Berat molekul kolagen 130 kDa .
PENGUJIAN AKTIVITAS DAYA ANALGETIK EKSTRAK n-HEKSANA, ETIL ASETAT DAN ETANOL DAUN KANGKUNG PAGAR (Ipomoea carnea Jacq) Qurotulaeni, Engkun; Setyahadi, Siswa; Simajuntak, Partomoan
Jurnal Buana Farma Vol. 4 No. 3 (2024): Jurnal Buana Farma : Jurnal Ilmiah Farmasi
Publisher : Fakultas Farmasi Universitas Buana Perjuangan Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36805/jbf.v4i3.1135

Abstract

Pain is unpleasant symptom and feeling in of the organs, caused by tissue damage such as inflammatory conditions, infection, or related to muscle spasms. Previous research, is known that leaves Ipomoea carnea can inhibit prostaglandins which are pain mediators. This research aims to determine the activity of analgesic leaves Ipomoea carnea. The leaves Ipomoea carnea macerated with 96% ethanol and partitioned with n-hexane and ethyl acetate. The three extracts tested on male white mice (Mus musculus) with a weight of 25-30 g. The research method used is the writhing test. The test animals were divided into 11 groups. Group 1 (Na CMC 0.5% positive control), Group 2 (Paracetamol as a comparison), Group 3-5 (Ipomoea carnea ethanol extract doses of 50 mg, 100 mg and 200 mg/Kg BW), Groups 6-8 (ethyl acetate extract of Ipomoea carnea leaves doses of 50 mg, 100 mg and 200 mg/kb BW) and groups 9-11 (n-hexane extract of Ipomoea carnea leaves dose 50 mg, 100 mg and 200 mg/kb BW). The pain inducer 0.5% acetic acid intraperitoneally. Observations were carried out for 1 hour every 5 minutes and the percentage of analgesic power was calculated. Ethanol extract provides effect than ethyl acetate and n-hexane extracts, dose of 50 mg/Kg BW 62.4%; 100 mg/Kg BW 70.42% and 200 mg/Kg BW 69.15%. The significant value of the Mann-Whitney test is 0.008 from the test sample against the positive control so it can be concluded that there is analgesic activity from the ethanol extract of Ipomoea carnea leaves.
Formulasi Masker Peel Off Gel dari Kombinasi Minyak Atsiri Sereh dan Cengkeh untuk Menghambat Bakteri Penyebab Jerawat Manus, Noriko; Taurhesia, Shelly; Setyahadi, Siswa; Manus, Widya Christine
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.11497

Abstract

Acne is a chronic skin disease involving inflammation of the pilosebaceous units with the main bacteria Staphylococcus epidermidis and Propionibacterium acnes has prompted the creation of cosmetic masks containing antibacterial agents. This study aims to evaluate the antibacterial activity of Lemongrass and Clove essential oils and to determine the synergistic effects of combining these essential oils in a peel-off gel mask formulation. The study was conducted using the liquid dilution method to determine the minimum inhibitory concentration and the disk diffusion method to test the inhibition zone diameter. Additionally, tests are conducted to determine the antibacterial activity against Propionibacterium acnes and Staphylococcus epidermidis, as well as to analyse the stability of the product both chemically and physically during storage. The results of the study showed that (1) the formulation containing a combination of 25% lemongrass and 18% cloves showed high inhibitory power and good stability, but caused moderate irritation; (2) lemongrass and clove essential oils were effective against Propionibacterium acnes and Staphylococcus epidermidis, but it was recommended to reduce the active ingredients to prevent potential irritation.
Antibacterial Activity of Kecombrang (Etlingera elatior) Stems Against Skin Infection-Causing Bacteria Adini, Syilvi; Kumala, Shirly; Setyahadi, Siswa
Sciences of Pharmacy Volume 4 Issue 3
Publisher : ETFLIN Publishing House

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Abstract

Skin infections caused by Staphylococcus aureus, Staphylococcus epidermidis, and Propionibacterium acnes often exhibit resistance to conventional treatments. This issue has led to the exploration of medical plants, such as kecombrang stems (Etlingera elatior), which are known for their antibacterial properties. This study aimed to evaluate the antibacterial activity of kecombrang stem and to identify its active compounds. The methanolic extract of kecombrang stems was tested against the three bacteria using the disc diffusion method at concentrations of 80%, 40%, and 20%. The Minimum Inhibitory Concentration (MIC) was determined using the microdilution method and an ELISA reader. TLC-Bioautography was employed to identify the antibacterial compounds present in the extract. The methanol extract of kecombrang stems exhibited antibacterial activity against S. aureus, S. epidermidis, and P. acnes, with an inhibition zone diameter ranging from 9.23 ± 0.472 to 25.53 ± 0.378 mm. The MIC results showed that the minimum concentration of 78.12 ppm could inhibit the growth of S. aureus by 33.74%, S. epidermidis by 14.45%, and P. acnes by 3.5%. The results of TLC Bioautography analysis indicate that flavonoids exhibit antibacterial properties. The kecombrang stem has the potential to serve as an antibacterial agent against bacteria that cause skin infections.
Co-Authors Adini, Syilvi Churiyah deden rosid waltam Febrina, Melia Heri Hermansyah Herman Suryadi Irvan Herdiana Manus, Widya Christine Noriko Manus, Noriko Ofa Suzanti Betha Partomuan Simanjuntak Qurotulaeni, Engkun Reubun, Yonathan Tri Atmodjo S, Partomuan Shirly Kumala Simajuntak, Partomoan Taurhesia, Shelly