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PENGARUH APLIKASI BIONEMATISIDA PURPUREOCILLIUM LILACINUM TERHADAP KOMUNITAS NEMATODA PADA LAHAN JAMBU KRISTAL Dewi, Ni Kadek Emi Sintha; Zahra, Fatimah Az; Swibawa, I Gede; Suharjo, Radix; Fitriana, Yuyun; Maryono, Tri; Lestari, Puji; Helina, Selvi; Aulia, Shifa Veronica; Wardiyani, Wardiyani; Marlinawati, Fransiska Dina; Basuki, Muh.; Ratdiana, Ratdiana
AGRICA Vol. 18 No. 1 (2025): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v18i1.5728

Abstract

Crystal guava (Psidium guajava var. crystal) is a high-value horticultural commodity, but its production in several regions has declined due to infestations by plant-parasitic nematodes. This study aimed to evaluate the effects of a bionematicide formulated with Purpureocillium lilacinum on the composition and diversity of soil nematode communities in crystal guava cultivation. Assessments were conducted on nematode abundance, absolute frequency, prominence value, and diversity metrics, including the Shannon index, evenness, dominance, and species richness, before and after the application of compost and bionematicide treatments. The results revealed the presence of 11 nematode genera, with Aphelenchus being the most dominant. Both compost and bionematicide treatments led to a decline in most genera, except Aphelenchoides, which increased in both abundance and prominence, and Rhabditis, which showed an increase in abundance but a reduction in ecological value. Post-treatment measurements indicated decreases in diversity, evenness, and richness indices, alongside an increase in dominance, particularly following compost application. These findings suggest that while P. Lilacinum is effective in targeting certain nematode groups; however, it may also reduce overall nematode community diversity, potentially leading to the dominance of the Aphelenchus genus. Thus, its application should be accompanied by ecological impact assessments to ensure the long-term sustainability of soil ecosystems.
Pendampingan PP Gapsera Sejahtera Mandiri Dalam Pengembangan Agens Hayati Sebagai Penerapan Budidaya Padi Tanpa Pestisida Maryono, Tri; Helina, Selvi; Lestari, Puji; Dewi, Ni Kadek Emi Sintha
International Journal of Community Service Learning Vol. 9 No. 2 (2025): May
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijcsl.v9i2.87551

Abstract

Masalah Perkumpulan Poktan (PP) Gapsera Sejahtera Mandiri adalah kelompok tani yang aktif dalam budidaya padi ramah lingkungan dan bebas residu pestisida. Namun, produksi padi di kelompok ini menghadapi kendala serius akibat serangan penyakit virus kerdil rumput padi dan kerdil hampa yang ditularkan oleh wereng batang coklat (WBC). Pengabdian ini bertujuan untuk melakukan pendampingan dalam perbanyakan massal agens hayati Trichoderma untuk menangani vektor WBC. Pengabdian ini menggunakan metode pendekatan institusional dan partisipatif. Selain itu, small laboratory dibuat pada lokasi PP Gapsera Sejahtera Mandiri untuk mendukung perbanyakan massal agens hayati. Pengumpulan data dilakukan melalui wawancara, kuesioner, dan observasi langsung agar mempermudah pemberian umpan balik selama kegiatan. Hasil pengabdian di PP Gapsera Sejahtera Mandiri, berlangsung lancar dan kondusif. Nilai pre-test dan post-test petani terkait agens hayati menunjukkan peningkatan signifikan sebesar 82,28%. Kegiatan pengabdian ini memberikan dampak signifikan pada peningkatan pemahaman dan keterampilan petani PP Gapsera Sejahtera Mandiri dalam memperbanyak agens hayati.
Morphological Identification of Balinese Caulerpa Seaweeds Dewi, Ni Kadek Emi Sintha; Wirawan, I Gede Putu; Suada, I Ketut
Jurnal Biologi Tropis Vol. 24 No. 2b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2b.7858

Abstract

Bulung boni and bulung anggur are species of Caulerpa seaweeds commonly found along the coastline of Serangan Island, Bali. Although these species have been widely utilized in various ways, formal species identification of these two macroalgae in Bali had not been conducted prior to this study. This research aims to characterize the morphological structures of bulung boni and bulung anggur through detailed morphological examination. Identification keys and previous studies were used to assist in the morphological identification process. The findings revealed that bulung boni shares morphological characteristics with Caulerpa cylindracea, while bulung anggur is morphologically comparable to Caulerpa macrophysa. These results provide valuable insights into the species composition of seaweeds in the region and contribute to a better understanding of local biodiversity.
Uji Efektivitas Trichoderma asperellum dalam Pengendalian Nematoda Puru Akar (Meloidogyne spp.) Secara In Vitro Indriyani, Sisi; Swibawa, I Gede; Dewi, Ni Kadek Emi Sintha; Ginting, Cipta
Jurnal Proteksi Agrikultura Vol. 3 No. 1 (2026): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1315-20

Abstract

Root-knot nematodes (Meloidogyne spp.) are significant pests that damage the root systems of plants and are difficult to control using conventional methods. The use of chemical nematicides carries the risk of environmental contamination, so environmentally friendly control alternatives are needed, one of which is using the biological agent Trichoderma asperellum. This study aims to determine the effect of spore concentration and exposure duration of T. asperellum on the hatching of Meloidogyne spp. eggs in vitro. The research was conducted at the Plant Pest Science Laboratory and the Biotechnology Laboratory of the Faculty of Agriculture, University of Lampung, from May to July 2025. The experiment used a completely randomized design (CRD). The first experiment tested five concentrations of spore suspension dilutions (10-1, 10-2, 10-3, 10-5, and a control), while the second experiment tested exposure durations of 10, 15, 30, and 60 minutes. The results showed that a concentration of 10-2 (2.7 × 105 spores/mL) was able to inhibit egg hatching by 50.90%, which was higher than the other concentrations. In the duration test, juvenile mortality increased over time, reaching 85.37% at 10 minutes and 99.83% at 60 minutes. In conclusion, a 10-2 concentration with a minimum exposure of 30–60 minutes is effective in reducing the hatching rate of Meloidogyne spp. eggs and has the potential to be applied as an environmentally friendly biological control.