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All Journal Journal of Tropical Plant Protection Agrica: Journal of Sustainable Dryland Agriculture
Ratdiana, Ratdiana
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Agriculture, Biological Sciences & Forestry Chemistry

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PENGARUH APLIKASI BIONEMATISIDA PURPUREOCILLIUM LILACINUM TERHADAP KOMUNITAS NEMATODA PADA LAHAN JAMBU KRISTAL Dewi, Ni Kadek Emi Sintha; Zahra, Fatimah Az; Swibawa, I Gede; Suharjo, Radix; Fitriana, Yuyun; Maryono, Tri; Lestari, Puji; Helina, Selvi; Aulia, Shifa Veronica; Wardiyani, Wardiyani; Marlinawati, Fransiska Dina; Basuki, Muh.; Ratdiana, Ratdiana
AGRICA Vol. 18 No. 1 (2025): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v18i1.5728

Abstract

Crystal guava (Psidium guajava var. crystal) is a high-value horticultural commodity, but its production in several regions has declined due to infestations by plant-parasitic nematodes. This study aimed to evaluate the effects of a bionematicide formulated with Purpureocillium lilacinum on the composition and diversity of soil nematode communities in crystal guava cultivation. Assessments were conducted on nematode abundance, absolute frequency, prominence value, and diversity metrics, including the Shannon index, evenness, dominance, and species richness, before and after the application of compost and bionematicide treatments. The results revealed the presence of 11 nematode genera, with Aphelenchus being the most dominant. Both compost and bionematicide treatments led to a decline in most genera, except Aphelenchoides, which increased in both abundance and prominence, and Rhabditis, which showed an increase in abundance but a reduction in ecological value. Post-treatment measurements indicated decreases in diversity, evenness, and richness indices, alongside an increase in dominance, particularly following compost application. These findings suggest that while P. Lilacinum is effective in targeting certain nematode groups; however, it may also reduce overall nematode community diversity, potentially leading to the dominance of the Aphelenchus genus. Thus, its application should be accompanied by ecological impact assessments to ensure the long-term sustainability of soil ecosystems.
Soil Fungus and Bacteria Antagonism Test to Control Phytophthora nicotianae in The In Vitro Istifada, Valia; Syib’li, Muhammad Akhid; Sektiono, Antok Wahyu; Ratdiana, Ratdiana; Limin, Atfritedy
Journal of Tropical Plant Protection Vol. 3 No. 2 (2022)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2022.003.2.5

Abstract

This study aims to determine the potential of antagonistic fungi and bacteria as controlling agents for Phytophthora nicotianae. The research was conducted at the Laboratory of Biological Control 1, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Brawijaya, Malang. The method used is soil sampling, isolation, purification, identification and in vitro antagonism test. The antagonism test is done by the dual culture method. This study used a completely randomized design for eight treatments, including the control and each treatment was repeated four times. In this study, the antagonism test results were obtained between antagonistic fungi and Phytophthora nicotianae, which Penicillium sp. (PEG01) had the highest percentage of inhibition, and the one with the lowest total diameter was Trichoderma sp. (TRC01). Moreover, the antagonism test results were obtained between antagonistic bacteria and Phytophthora nicotianae, which bacteria C had the highest percentage of inhibition and the lowest total diameter.
Co-Authors Akmalia, Alien Aulia, Shifa Veronica Basuki, Muh. Dewi, Ni Kadek Emi Sintha Helina, Selvi I GEDE SWIBAWA, I GEDE Istifada, Valia Limin, Atfritedy Marlinawati, Fransiska Dina PUJI LESTARI Radix Suharjo Sektiono, Antok Wahyu Syib’li, Muhammad Akhid Tri Maryono Wardiyani, Wardiyani Yuyun Fitriana Zahra, Fatimah Az