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Microbiological Assessment of Fresh Expressed Breast Milk on Room Temperature at Dr. Soetomo Hospital Neonatal Unit Widjaja, Nur Aisyah; Hardiyani, Kartika; Hanindita, Meta Herdiana; Irawan, Roedi
Folia Medica Indonesiana Vol. 55 No. 1 (2019): March
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.9 KB) | DOI: 10.20473/fmi.v55i1.24346

Abstract

Storing EBM at room temperature in several hours before consuming, frequently found in Indonesia. Based on Academy of Breastfeeding Medicine guidelines EBM can last for 6 to 8 hours in room temperature (25oC or 77oF). However, currently there hasn't been study in tropical country especially Indonesia for the guidelines. This study aimed to assess microbiological quality of EBM on room temperature, including bacterial growth and major bacterial found on EBM for health care and society recommendations. An observational study of 30 expressed breast milk samples provided by 30 healthy women with term baby below 6 month old. The samples were kept sterile and laid at plates for 0 hours, 2 hours, 4 hours and 6 hours in room temperature (26°-32° C) and used drop plate technique on several culture media. Data was analyzed by Chi-square and paired sample T-test. Thirty of unheated fresh EBM from 30 lactating mothers were stored at room temperature, examined for the degree of bacterial contamination at 0 hour, 2 hours, 4 hours, and 6 hours. All the EBM samples were contaminated at 2 hour. Bacterial species identified was Coagulase-negative Staphylococcus (CNS), Escherichia coli, Klebsiella pneumoniae and Streptococcus faecalis, range of growth 109 cfu/ml-63 x 109 cfu/mm3 after 6 hour of storage. The EBM exposed at room temperature (30-36 0C) for more than two hour reduce the quality and do not recommended to be given to the infants. 
Alternative Milk Options for Infants with Cow's Milk Allergy: A Systematic Review of Goat's Milk, Plant-Based Milk, and Partially Hydrolyzed Protein Wija, Ida Bagus Eka Utama; Angelica, Chatrine; Hardiyani, Kartika; Felicia, Mildi; Firmansyah, Gogma; Nandya, Galuh; Tampubolon, Christine Handayani
Jurnal KESANS : Kesehatan dan Sains Vol 4 No 12 (2025): KESANS: International Journal of Health and Science
Publisher : Rifa'Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54543/kesans.v4i12.426

Abstract

Introduction: Cow's milk allergy (CMA) is the most common food allergy occurring in the first year of life. Restriction of cow's milk and dairy products results in decreased macro- and micronutrient intake, especially in infants who are not breastfed. Therefore, alternative milk supplements for supplemental nutrition in CMA must be considered. Methods: This systematic review used PRISMA guidelines with the PICO search strategy, focusing on RCTs, clinical trials, and observational studies. A comprehensive search was conducted to obtain articles published in English between 2010 and 2025 using ScienceDirect, PubMed, SagePub, and Scopus. Result and Discission: Results A total of 2010 publications were retrieved using relevant search terms. After a rigorous three-stage review, nine studies were selected based on inclusion criteria and deemed suitable for in-depth analysis. These studies addressed tolerance and allergic reactions or cross-reactivity following administration of goat's milk, soy isolate, rice isolate, and partially hydrolyzed formula. Conclusion: In infants with CMA, goat's milk can trigger cross-reactivity. Partially hydrolyzed formulas can be an option for oral food challenges to assess tolerance. Plant-based formulas, particularly rice isolate, are the best choice, especially for those with non-severe CMA and CMA who cannot tolerate extensively hydrolyzed and amino acid-containing formulas.