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All Journal Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Pharmaceutical Science and Technology Indonesian Journal of Biotechnology JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Jurnal Farmasetis Acta Holistica Pharmaciana
Arimurni, Dewa Ayu
Sekolah Tinggi Farmasi Mahaganesha
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Other

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Formulation of Medium Viscosity Chitosan-Pectin –MJ Protein Nanoparticles Conjugated with Anti-Ep-CAM and Its Cytotoxicity Against T47D Breast Cancer Cell Lines Anggun Feranisa; Dewa Ayu Arimurni; Hilda Ismail; Ronny Martien; S. Sismindari
Indonesian Journal of Biotechnology Vol 20, No 1 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.516 KB) | DOI: 10.22146/ijbiotech.15283

Abstract

Chitosan nanoparticle could become potential formula to protect protein degradation during therapy,since chitosan nanoparticles have “proton sponge hypothesis” mechanism on its protection. Chitosan and pectinis used as basic formula of drug delivery because of its biodegradable and biocompatible properties. Chitosanpectin nanoparticles can be formulated by polyelectrolit complex. EpCAM showed excessive expression inepithelial cancer cells thus can be used as a therapeutic biomarker. MJ protein, a Ribosome-Inactivating Proteins(RIPs) isolated from Mirabilis jalapa L had a higher cytotoxicity on malignant cells than normal cells. MJ proteinneed to be formulated to protect from proteosome degradation in endosome. The aim of this research was todevelop MJ protein-chitosan-pectin nanoparticles and conjugated with anti EpCAM for breast cancer therapy.Mj protein was extracted from M.jalapa leaves. RIPs activity was assayed by supercoiled DNA cleavage. MJprotein were loaded into chitosan nanoparticles using medium viscous chitosan and pectin as cross-linker withpolyelectrolit complex method. Anti EpCAM was conjugated to MJ protein-chitosan-pectin nanoparticles bycarbodiimide reaction and characterized for its entrapment efficiency, morphology by transmission electronmicroscope, particles size, and zeta potential. MJ protein nanoparticles conjugated anti EpCAM and withoutanti EpCAM were cytotoxicity assayed toward T47D and Vero cell lines. MJ protein was able to cleave thesupercoiled DNA into linear and nicked-circular ones. The nanoparticles optimal concentration of mediumviscous chitosan: MJ protein: pectin was 0.01%: 0.01%: 1% (m/v). A high entrapment efficiency of MJ proteinnanoparticles was 98.97 ± 0.07%. Morphology nanoparticles showed an amorphic structure with 200.00 nmparticles size. The nanoparticles conjugated anti EpCAM showed average particles size 367.67nm, polydispersityindex 0.332, and zeta potential +39.97mV. MJ protein-chitosan-pectin nanoparticles conjugated anti EpCAMand unconjugated both had higher cytotoxicity with the IC50 57.64 μg/mL and 46.84 μg/mL respectivelyagainst T47D and 99.38 μg/mL and 111.34 μg/mL against Vero cell lines compared to MJ protein with IC50 of3075.61 μg/mL against T47D and 3286.88 μg/mL against Vero cell lines. Both MJ protein-nanoparticles couldincrease the cytotoxicity effects about 50 times compared to the unformulated MJ protein activity, howeverhad less specificity toward T47D and Vero cell lines.
Development and optimization of Curcuma longa Linn. oleoresin non-aqueous gel for transdermal delivery Dewa Ayu Arimurni; Made Dwi Pradipta Wahyudi S; Erika Yuda Colatama
Pharmaciana Vol 12, No 1 (2022): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (646.828 KB) | DOI: 10.12928/pharmaciana.v12i1.22285

Abstract

A long-term oral administration of NSAID and DMARD on rheumatoid arthritis (RA) treatment may cause gastritis, kidney, and cardiovascular disorder. One of the alternative therapies that have been investigated is by using herbal medicine such as Curcuma longa Linn. which contains curcumin and essential oils. Even though both compounds are quite effective in treating RA, poor aqueous solubility and low intestinal absorption limit their oral bioavailability. To overcome these drawbacks, transdermal delivery was chosen as an alternative route of administration. This study was aimed to formulate the Curcuma longa Linn. oleoresin into a transdermal non-aqueous gel system using Carbopol 934 and low substituted hydroxypropyl cellulose (4.25:0.75 %) as the gelling agent. In this study, multiple solvents (PEG 400, PG, glycerin, ethanol, and tween 20) were used in the system. The solvents were chosen based on their ability to dissolve the gelling agents. Optimization was done using a simplex lattice design based on the physical characteristics (viscosity, pH, spreadability, and adhesivity) of the prepared gel. The system with the optimum concentration of PEG 400 and PG was then observed for its stability and in vitro transport through snakeskin membrane using Franz diffusion cell with PBS pH 7.4 as acceptor medium. The optimal formula was comprised of 75% PEG and 25% PG which has a viscosity of 6.34+0.19 dPa.s, adhesivity of 6.05+0.11 seconds, pH of 5.16+0.09, spreadability of 6.94+0.06 cm, and quite stable after freeze-thaw cycling test, whilst around 26.85% curcumin was diffused through the membrane (flux = 0.084 mg.cm-2) after 2 hours. It can be concluded that the Curcuma longa Linn. oleoresin can be formulated into a non-aqueous gel system, which showed a fair gel physical characteristic with good stability and ability to permeate across the skin membrane, and is promising to be further developed as an alternative for RA treatment.
Formulasi dan Evaluasi Gel Nanopartikel Perak Citra Ariani Edityaningrum; Artika Tri Oktafiani; Lina Widiyastuti; Dewa Ayu Arimurni
Indonesian Journal of Pharmaceutical Science and Technology Vol 9, No. 2, 2022
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v9i2.35343

Abstract

Nanopartikel perak diketahui dapat menembus membran sel bakteri penyebab jerawat dan menyebabkan kerusakan struktural yang berakibat pada kematian sel bakteri. Nanopartikel perak perlu diformulasikan dalam bentuk gel agar memudahkan dalam penggunaannya. Penelitian ini dilakukan untuk mensintesis nanopartikel perak dan memformulasikannya dalam bentuk gel. Sintesis nanopartikel perak dilakukan menggunakan metode reduksi kimia dengan reduktor natrium sitrat dan penstabil gelatin. Nanopartikel perak kemudian diformulasikan dalam sediaan gel dengan variasi konsentrasi HPMC 7%, 10%, dan 15%. Hasil karakterisasi menunjukkan telah terbentuk nanopartikel perak berdasarkan panjang gelombang maksimal yang terbentuk yaitu 430,2 nm, absorbansi 0,297, ukuran partikel 157,73±15,03 nm, indeks polidispersitas 0,328±0,032, dan morfologi bulat. Berdasarkan hasil analisis, dapat disimpulkan bahwa formula 3 dengan konsentrasi HPMC 15% merupakan formula terbaik dengan sifat fisik berupa nilai viskositas 3931,75 ± 92,383 cps, diameter sebar 6,41 ± 0,06 cm dan daya sebar 22,78 ± 0,221 g.cm/detik, daya lekat 59,43 ± 1,158 detik, serta nilai pH 5,81 ± 0,04.
Formulation and Characterization of Carbamazepine Chitosan Nanoparticle Citra Ariani Edityaningrum; Ariza Nur Zulaechah; Widyasari Putranti; Dewa Ayu Arimurni
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 9 No. 2 (2022): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v9i22022.146-154

Abstract

Background: Carbamazepine is an antiepileptic drug used to treat trigeminal neuralgia and pain associated with neurological disorders. The drug belongs to class II of the Biopharmaceutical Classification System (BCS), which has low solubility. Hence, dissolution is a rate-limiting step. Objective: This study aimed to determine the best formula for carbamazepine nanoparticles based on physical characteristics and determine the effect of chitosan and Na-TPP concentration variation on nanoparticle characterization. Methods: The carbamazepine chitosan nanoparticles were prepared using ionic gelation method with a concentration of 0.1% w/v carbamazepine and the ratio of chitosan and Na-TPP concentrations of 0.2%:0.1% (F1), 0.2%:0.2% (F2), and 0.3%:0.1% w/v (F3). The parameters evaluated included particle size, polydispersity index, zeta potential, particle morphology, and entrapment efficiency. Statistical analysis was conducted on the evaluation data using One Way ANOVA. Results: The results showed that the effect of increasing the concentration of chitosan reduced particle size (p<0.05), increased zeta potential (p<0.05), and had no effect on the value of entrapment efficiency (p>0.05). Furthermore, F3 had a particle size of 169.8±13.71 nm with a polydispersity index of 0.378±0.02, the zeta potential of +28.80±2.44 mV, entrapment efficiency of 84.3±7.50%, and spheric particle morphology which was measured using Transmission Electron Microscope (TEM). Conclusion: Therefore, F3 with the ratio of chitosan and Na-TPP concentrations of 0.3%:0.1% was the formula that provided the best characteristics of chitosan carbamazepine nanoparticles.
Formulation optimization and wound healing activity of Vitex trifolia L leaf extract loaded chitosan hydrogel film on hyperglycemic rats Made Dwi Pradipta Wahyudi S; Dewa Ayu Arimurni; Komang Angelita Safira; Erika Yuda Colatama; Putu Bisma Duta Valenrika
Pharmaciana Vol 12, No 3 (2022): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (617.307 KB) | DOI: 10.12928/pharmaciana.v12i3.25035

Abstract

Decreased blood supply, high blood sugar level, and a possibility of bacterial infection in diabetic wounds pose risk for limb loss and mortality. Therefore, proper wound care is needed to improve the quality of life of diabetic patients. Vitex trifolia L (Legundi) extract is reported to have antibacterial and antioxidant activity that enhances cell proliferation and migration. The antimicrobial and hemostatic properties of chitosan film are viewed as an ideal material for enhancing wound healing. The film should retain its integrity and flexibility while used on the skin, therefore chitosan was combined with PVA and PVP K30 to improve its quality. The objectives of this study were to optimize the concentration of chitosan, PVP K30, and PVA towards the Legundi extract film properties, and to evaluate its wound healing activity on hyperglycemic mice using an incision wound model. In this study, the film’s compositions were optimized using a simplex lattice design, and the effects of its components on their characteristics, such as thickness, weight, folding endurance, swelling rate, and swelling index, were evaluated. BALB/c Mice were divided into three groups (Group I, Group II, Group III) which were treated with normal saline, placebo film, and Legundi film respectively once daily for 8 days. The result suggested that chitosan and PVA were responsible for affecting the film’s thickness, weight, and folding endurance, whilst PVP K30 was the dominant factor in increasing the swelling index and rate of the film. The optimum formula of Legundi extract films consists of 1.15 %(b/v) chitosan, 1.25 %(b/v) PVP K30, and 1.6 %(b/v) PVA. The animal treated with Legundi extract film have higher wound closure compared to the control and placebo group four days after wound incision (p<0.05). Thus, Legundi extract film was a potential dressing to treat a diabetic wound.
Optimasi Komposisispan 60 dan Tween 80 dalam Sediaan Body Cream Ekstrak Umbi Bit Menggunakan Metode Simplex Lattice Design Made Dwi Wahyudi S.; Ni Komang Eni Astari; Ni Putu Ratna Mulia Adnyani; Dewa Ayu Arimurni; Elisabeth Oriana Jawa la
Jurnal Farmasetis Vol 12 No 4 (2023): Jurnal Farmasetis: November 2023
Publisher : LPPM Sekolah Tinggi Ilmu Kesehatan Kendal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32583/far.v12i4.1405

Abstract

Umbi bit (Beta vulgaris), salah satu tanaman yang banyak tumbuh di Indonesia, diketahui memiliki aktivitas antioksidan. Penelitian ini bertujuan untuk mengoptimasi komposisi span 60 dan dan tween 80 pada krim ekstrak etanol umbi bit. Optimasi konsentrasi span 60 serta tween 80 dilakukan dengan metode Simplex Lattice Design menggunakan Design Expert 13.0. Sediaan yang dibuat dievaluasi karakteristiknya yang meliputi organoleptis, homogenitas, daya sebar, pH, dan daya lekat. Hasil penelitian menunjukkan bahwa perbedaan komposisi span 60 dan tween 80 dapat mempengaruhi kualitas fisik sediaan body cream ekstrak umbi bit yang meliputi daya sebar, daya lekat dan pH. Formula optimum krim ekstrak etanol umbi bit diketahui memiliki komposisi 2,34% span 60 dan 2,86% Tween 80 dengan diameter sebar sebesar 5,31 cm; pH 7,05; daya lekat 0,48 s dan viskositas 96,67 dPa.s.
PENDEKATAN SIMPLEX LATTICE DESIGN PADA FORMULASI WOUND DRESSING GEL PENTOXIFYLLINE DENGAN KOMBINASI GELLING AGENT HPMC DAN CHITOSAN Dewa ayu Arimurni; Made Dwi Pradipta Wahyudi S; Gede Ngurah Ary Zega Widyartha; Luh Gede Tina Sujayanti; Gracia Isabel Baptista Soares
Acta Holistica Pharmaciana Vol 2 No 2 (2020): Acta Holistica Pharmaciana
Publisher : School of Pharmacy Mahaganesha (Sekolah Tinggi Farmasi Mahaganesha)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pentoxifylline terbukti memiliki khasiat sebagai penyembuh luka seperti ulkus diabetik. Pentoxifylline pada penelitian inidiformulasikan dalam bentuk sediaan gel dengan kombinasi basis HPMC dan Chitosan. Penggunaan kombinasi ini diketahui akan menghasilkan gel dengan sifat fisik yang lebih baik daripada penggunaan tunggal. Penelitian ini bertujuan untuk meneliti pengaruh variasi HPMC dan chitosan terhadap sifat fisik gel dan stabilitas fisik gel pentoxifylline selama penyimpanan, serta mencari konsentrasi HPMC dan Chitosan yang optimum. Sediaan gel dibuat dengan konsentrasi pentoxifylline sebesar 1% dengan basis HPMC dan chitosan. Komposisi HPMC dan chitosan ditentukan melalui proses skrining dan dioptimasi menggunakan metode Simplex Lattice Design dengan perangkat lunak Design Expert trial version 10.0.3. Masing-masing formula diuji sifat fisiknya yang meliputi pH, viskositas, daya sebar, dan daya lekat. Formula optimum gel pentoxifylline terdiri dari 3,5% HPMC dan 1,5% chitosan. Hasil uji sifat fisik gel pentoxifylline optimum menunjukkan bahwa sediaan yang diperoleh homogen dengan nilai pH 5, viskositas 266,67 dPa.S, daya sebar 43,33 cm.g/menit, dan daya lekat 2,16 detik. Gel pentoxifylline stabil selama 7 siklus pengujian meliputi daya lekat, viskositas, daya sebar dan daya lekat, gel stabil ditandai dengan P value tidak berbeda signifikan.
Optimization of self-nano emulsifying drug delivery system of rifampicin for nebulization using cinnamon oil as oil phase Ricardo, I Komang Aan Adi; Arimurni, Dewa Ayu
Pharmaciana Vol. 14 No. 3 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i3.28892

Abstract

Lung delivery can overcome the problems related to the effectiveness of tuberculosis treatment by increasing the drug concentration at the target site. Rifampicin as the first-line antibiotic for tuberculosis has low water solubility and is unstable in gastric which hinders its effectiveness. Self-nanoemulsifying drug delivery system (SNEDDS) is a strategy known to improve the solubility and stability of such drugs. This study aimed to obtain the optimum formula of rifampicin SNEDDS intended for lung nebulization using essential oil as an oil phase. Several essential oils are known to have effective antibacterial on Mycobacterium tuberculosis. However, a high capability to solubilize the drug is required for SNEDDS formulation. Cinnamon oil, tween 80, and transcutol P were chosen as SNEDDS components for optimization using a D-optimal mixture based on the physicochemical characteristics. The optimum formula comprised 12.65% cinnamon oil, 75.00% tween 80, and 12.35% transcutol P which dispersed easily to form a highly transparent emulsion in normal saline under 1 minute. Upon dilution with saline, the optimal SNEDDS can produce a homogenous nanometer droplet (169.2±19.771 nm, PDI of 0.258±0.070) with acceptable pH for lung administration. It also has a viscosity similar to water (0.94±0.01 cP) which allows it to be nebulized easily (aerosol output rate of 0.14±0.02 g/min). Although the diluted SNEDDS has a zeta potential of -2.533±0.268 mV, it was stable for up to 4 hours during the nebulization. These results indicate the potential of cinnamon oil-based rifampicin SNEDDS to be an alternative in the pulmonary delivery of rifampicin via nebulization.
Formulations and Antibacterial Activity of Shallot (Allium cepa L.) Peel Extract Patch against Streptococcus pyogenes Azizah, Siwi Nur; Ste, Luay Banna; Maulida, Khansa Syahira; Zahirah, Nadrah Adinda; Nisa, Salsabila Naura; Sri Mulyaningsih; Arimurni, Dewa Ayu; Edityaningrum, Citra Ariani
JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Vol. 12 No. 2 (2025): JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jfiki.v12i22025.247-255

Abstract

Background: Bacterial pharyngitis is an inflammatory condition in the back of the throat caused by Streptococcus pyogenes. Patients are often prescribed antibiotics and antiinflammatories to alleviate pain and discomfort while reducing bacterial growth in the throat. However improper and prolonged use of antibacterial and antiinflammatory agents increases the risk of bacterial resistance and side effects. An often discarded Shallot (Allium cepa L.) peel rich in flavonoids with great antibacterial and anti-inflammatory properties is potentially used as an alternative treatment for bacterial pharyngitis. Objective: This study aimed to develop shallot peel extract as an antibacterial against Streptococcus pyogenes. Methods: Patch was formulated with variations in extract concentration of 5% (F1), 10% (F2), and 15% (F3) to observe their influence on weight uniformity, thickness, folding endurance, surface pH, moisture content, and antibacterial activity using disc diffusion. Results: All formulations produce slightly heavy and thicker but uniform patches (CV<5%), surface pH suitable for application in the skin (4.6-4.9), flexible and durable patches with high folding endurance (> 300 folds), good moisture content (<10%) and moderate to strong antibacterial activity (inhibition zone diameter ranging from 9 to 13.67 mm). Variations in extract concentration in the formula significantly influenced the thickness, weight, folding endurance, and also the antibacterial activity of the patches. Higher concentrations of extract produce thicker and heavier patches but stronger antibacterial activity against pharyngitis pathogens. Conclusion: Therefore, antibacterial patches containing up to 15% shallot peel extract are potentially used as an alternative treatment for pharyngitis.
Co-Authors Anggun Feranisa Ariza Nur Zulaechah Artika Tri Oktafiani Azizah, Siwi Nur Citra Ariani Edityaningrum, Citra Elisabeth Oriana Jawa La Erika Yuda Colatama Gede Ngurah Ary Zega Widyartha Gracia Isabel Baptista Soares Hilda Ismail, Hilda Komang Angelita Safira Luh Gede Tina Sujayanti Made Dwi Pradipta Wahyudi S Maulida, Khansa Syahira Ni Komang Eni Astari Ni Putu Ratna Mulia Adnyani Nisa, Salsabila Naura Putu Bisma Duta Valenrika Ricardo, I Komang Aan Adi Ronny Martien S. Sismindari Sri Mulyaningsih Ste, Luay Banna Widiyastuti, Lina Widyasari Putranti Zahirah, Nadrah Adinda