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Program Studi Agroteknologi, Fakultas Pertanian, Universitas Slamet Riyadi Surakarta
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Social Sciences Other

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Journal : Proceeding of International Congress

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AVOID CONTAMINATION IN SOYBEAN (Glycine max, L. [Merrill]) MICROSPORES CULTURE -, Sumarmi
Proceeding of International Congress 2014: Challenges of Biotechnological Research in Food and Health
Publisher : Proceeding of International Congress

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Abstract

Microspore culture is done to obtain pure strains. The purpose of soybean microspore culture to obtainquality seeds. Two important step that must be done is isolation of microspores in starvation medium andsubculture into embryogenesis medium. Many factors contributing to the contamination of soybeanmicrospore culture. Contamination in the B medium temperature 34 0C is more common than 4 0C. Vulnerableto contamination because of embryogenesis medium rich in nutrients. Bacterial contaminationcan be caused by internal contaminants such as shape of the anther. Other internal contaminants thatcause diseases such as fungi Colletotrichum truncatum and Phakopsora pachyrhizi. Antagonistic fungiwhich contaminate cultures that Trichoderma spp., Alternaria spp., Fusarium spp. Handling of contaminationis done by selecting the appropriate methods in order to remain viable microspores. Sterilization soybeanflower buds with 20% Tween for 10 minutes and then rinsed with distilled water. Moreover sterilizationwith 4% Hg Cl2 and 10% NaOCl for 10 minutes, rinsed with distilled water times, followed by 96%alcohol for 1 minute, can press up to 70% contamination.Keywords: soybean, microspore, contamination
Co-Authors Afringga Rohmawan Angga Fitriadi Nugroho Arif Ramadhan Aslam, Benhar Awaludin Sinur Kholis Ayu Dita Rani Ayu Dita Rani Chanindra Dhimas Chanindra Dhimas Fiqie Saputra Dewi Ratna Nuhayati Diana Diah Utami Dina Nur Fitrian Fransiska Mardiyana haris asrori Lulus Fitriyani Merkuria Karyantina Prasetyo Bowo Riana Kusumasari Riris Dwi Puspita Risti Arina Lestari Saiful Bahri Saiful Bahri Santi Murdiahningsih Sartono Joko Santosa Sartono Joko Santoso Sholihah, Efi Nikmatu Sri Hardiatmi Swari Utami Dewi Triyono, Kharis wahyu agustina agustina Zuniar Amanza Pratama Putra