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Journal : PROSIDING SEMINAR KIMIA

THE POTENTIAL OF SECONDARY METABOLITES COMPOUNDS OF METHANOL EXTRACT STEM BARK MELOCHIA UMBELLATE AS ANTI-BACTERIAL Usman -; Nunuk Hariani S; Hanapi Usman; Ahyar Ahmad
PROSIDING SEMINAR KIMIA PROCEEDING OF INTERNATIONAL CONFERENCE 2015
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

A Study antibacterial essay of the methanol extracts of steam bark M.umbellate (Houtt) Stapf var. degrabrata (Paliasa). The results of this study showed that the methanol extract from the bark of M. umbellate (Houtt) Stapf var. degrabrata group of compounds containing alkaloids, flavonoids, triterpenoids, phenolics and saponins. Methanol extract from the bark of M. umbellate can inhibit the growth of bacteria Bacillus subtilis, Staphylococcus aureus, gram-negative bacteria as well as representing Escherichia coli, Salmonella thypi, and Pseudomonas aeruginosa representing gram-positive bacteria. At a concentration of 400 ppm of methanol extracts of these plants showed effective barrier against bacteria tested, with a zone of inhibition, respectively as follows: 12.67 mm against bacteria B. subtilis, 12.97 mm against bacteria, S. aureus, 11.86 mm against bacteria E. coli and 12.12 mm against bacteria S. thypi. However, methanol extract from the bark of
SENYAWA TURUNAN OLEANAN DARI KULIT BATANG Melochia umbellata (Houtt) Stapf var. Degrabrata K DAN BIOAKTIVITASNYA Usman -; Nunuk Hariani Soekamto; Ahyar Ahmad
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2014
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

Senyawa turunan oleanan 3-asetil-12-oleanen-28-oat diisolasi dari fraksi n-heksan kulit batang M.umbellata (Houtt) Stapf var. degrabrata K. Struktur molekul ditentukan melalui analisis data spektroskopi IR, NMR 1D dan NM R 2D ( 1 H, 13 C, DEPT, COSY, HMQC dan HMBC). Hasil uji bioakt ivitas menunjukkan bahwa senyawa tersebut bersifat toksik terhadap A. salina dengan nilai LC 50 361,93 μg/mL. Pada konsentrasi 1000 μg/ mL senyawa oleanan 3-asetil-12-o leanen-28-oat memberikan hambatan tertinggi terhadap bakteri B. subtilis dan jamu r C. albicans, dengan diameter zona hambat 15,8 mm dan 15,2 mm.