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All Journal Jurnal Sains dan Teknologi Meditory : The Journal of Medical Laboratory
Widiari, Ni Nyoman
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Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Environmental Science

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Potensi Cuka Salak Sibetan sebagai Disinfektan pada Alat Penatah Gigi Sangging dalam Upacara Potong Gigi “Metatah” Widiari, Ni Nyoman; Arini , Ni Putu Dewi Tata; Karta, I Wayan; Dwija Putra, I Gusti Ngurah
JST (Jurnal Sains dan Teknologi) Vol. 13 No. 3 (2024): Oktober
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jstundiksha.v13i3.84648

Abstract

Sterilisasi alat tatah dalam upacara Metatah masih menggunakan desinfektan kimia yang berpotensi menimbulkan efek samping bagi kesehatan dan lingkungan. Alternatif desinfektan alami berbasis bahan lokal seperti cuka salak Sibetan belum banyak diteliti, khususnya dalam konteks ini. Penelitian ini bertujuan untuk menganalisis efektivitas cuka salak Sibetan sebagai desinfektan alami dengan mengevaluasi kandungan fitokimia, aktivitas antioksidan, dan kemampuan antimikroba terhadap alat tatah. Penelitian menggunakan desain eksperimen laboratorium dengan pendekatan kuantitatif. Subjek penelitian melibatkan cuka salak konsentrasi 2% yang diuji pada empat alat tatah dan sampel kontrol menggunakan akuades dan alkohol 70%. Data dikumpulkan melalui uji total fenol, flavonoid, tanin, aktivitas antioksidan menggunakan DPPH, serta analisis Angka Lempeng Total (ALT) mikroorganisme pada alat tatah. Data dianalisis menggunakan metode statistik deskriptif dan inferensial. Hasil penelitian menunjukkan cuka salak memiliki efektivitas tinggi sebagai desinfektan alami. Kandungan fenol, flavonoid, dan tanin yang tinggi memungkinkan cuka salak secara signifikan mengurangi mikroorganisme hingga tingkat yang sebanding dengan alkohol 70%. Kesimpulan penelitian menegaskan bahwa cuka salak dapat menjadi alternatif desinfektan alami yang efektif dan aman, menjawab permasalahan kesehatan pada upacara Metatah. Penelitian ini mendukung pengembangan desinfektan berbasis bahan alami lokal yang ramah lingkungan dan berpotensi mendukung keberlanjutan praktik budaya sekaligus memperkuat nilai ekonomi lokal.
PHYTOCHEMICAL AND ANTIMICROBIAL ACTIVITY TESTS OF TURMERIC TINCTURE AS A DISINFECTANT FOR MEPANDES CEREMONIAL EQUIPMENT IN BALI Widiari, Ni Nyoman; Tata Arini, Ni Putu Dewi; Karta, I Wayan
Meditory : The Journal of Medical Laboratory Vol 12, No 1 (2024): Meditory, Volume 12 No. 1 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i1.3077

Abstract

Background: In the traditional Mepandes ceremony of Bali, one of the key practices involves the use of sangging equipment, including chisels, files, and sharpeners, to smoothen teeth surfaces. Typically, one set of sangging tools is shared among 3-10 individuals, rotating their use. This practice poses a potential risk of disease transmission. The current cleaning methods vary, with some using water and turmeric while others soak the equipment in 5% NaOCl. However, the use of water alone may not ensure proper hygiene, and NaOCl usage presents challenges such as equipment odor and potential health hazards.Aims: The objective of this study is to qualitatively analyze the phytochemical content, inhibitory activity, and total plate count in turmeric tinctures at concentrations of 5%, 10%, and 15% Methods: The tincture is prepared by soaking turmeric simplicia in 70% alcohol for 5 days at concentrations of 5%, 10%, and 15%. In this study, the negative control is 70% alcohol, the positive control is 5% NaOCl, and the treatment includes tinctures at 5%, 10%, and 15%. Phytochemical tests include flavonoid, tannin, saponin, terpenoid, and alkaloid assays. Antimicrobial activity is assessed through total plate count (TPC) and inhibition tests. The results The research results indicate the presence of tannin, flavonoids, and phenols in all three treatments. Based on the antibacterial tests and total plate count, all three tincture solutions can be used as disinfectants. However, for efficiency, a 5% concentration is recommended as it exhibits the same antibacterial strength as the 10% and 15% tinctures.Conclusions:The use of turmeric tincture treatment can be employed as a disinfectant for ceremonial tools since it can inhibit the growth of S. aureus bacteria, and its total plate count value matches that of the positive control.
Co-Authors Arini , Ni Putu Dewi Tata Dwija Putra, I Gusti Ngurah I Wayan Karta Tata Arini, Ni Putu Dewi