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All Journal PREDIKSI JURNAL BIOLOGI INDONESIA Jurnal Pemberdayaan: Publikasi Hasil Pengabdian kepada Masyarakat (JPM ITTC ) Jurnal Kajian Stratejik Ketahanan Nasional
Fatimah .
Fakultas Ilmu Budaya Universitas Brawijaya
Religion Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Social Sciences

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Journal : JURNAL BIOLOGI INDONESIA

 1 
Analisis Molekuler Piramida Gen Xa pada Progeni Padi Varietas Ciherang dan Inpari 13 ., Fatimah; Prasetiyono, Joko; Priyatno, Tri Puji; Yunus, Muhammad; Suhartini, Tintin; Ridwan, Iman; Baroya, Mushlihatun
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2161

Abstract

Bacterial leaf blight (BLB) is a major disease in Indonesian lowland rice.This research was undertaken to pyramid three BLB resistant genes xa5, Xa7 and Xa21 and one background BLB resistant gene Xa4 into Ciherang and Inpari 13 varieties. The donor parent Code (Xa4+Xa7) was crossed with Angke (Xa4+xa5) while Ciherang and Inpari 13 were crossed with IRBB21 (Xa21). Progenies were selected using marker assisted selection and yield component observation. Foreground selection was conducted using SSR and STS markers linked with the targeted genes in the F1 and DCF1 population. Individuals with triple positives Xa genes were screened for the presence of Xa4 gene as the background. Selected heterozygote plants in F1 Code x Angke, F1 Ciherang x IRBB21 and F1 Inpari 13 x IRBB21 were used to develop DCF1 population. Molecular analysis on DCF1 population through alleles of three BLB resistant genes xa5, Xa7 and Xa21 and one background BLB resistant gene Xa4 resulted 8 (2,6%) in DCF1 Ciherang and 13 (3,5%) in DCF1 Inpari 13. Yield component characters on F1 Code x Angke resulted significant in number of panicle. F1 Ciherang x IRBB21, F1 Inpari 13 x IRBB21 and DCF1 Ciherang resulted significant in weight of empty grain while DCF1 Inpari 13 resulted no significance in all of observed characters. Keywords: Rice, F1 Population, DCF1 Population, molecular marker, Xa gene 
Isolation and Disease Assessment of Xanthomonas oryzae pv. oryzae from Java Island and Pathogenic Assay on Near Isogenic Lines with Different Resistant Genes ., Fatimah; Priyatno, TP.; Fadlillah, SH.; ., Hermanto; Baroya, M.; ., Mahrup; ., Wawan; Sasongko, D.; Suryadi, Y.; Kadir, TS.
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.2104

Abstract

Bacterial leaf blight disease caused by Xanthomonas oryzae pv oryzae (Xoo) is one of the important bacterial diseases,which is very destructive to rice plant. In this study, seventy isolates of Xoo were collected from several regions inWest Java, Central Java, Yogyakarta and East Java provinces and ten isolates from ICABIOGRAD collection. Theaim of the study was to survey variation and distribution of the pathogen and to study the interactions between theisolates and near isogenic lines carrying specific genes for bacterial leaf blight resistance. Twenty Xoo isolates wererandomly chosen to assess the differential characteristics of ten near-isogenic rice lines in Indonesia. The resultsshowed that xa5 resistant gene was the highest effective against the majority of Xoo isolate, followed by Xa21, andXa7 combined with Xa4 as the background. These findings are useful for rice breeding programs in designing stablebroad-spectrum bacterial blight resistant rice cultivars.Key words: Xanthomonas oryzae, resistant gene, Near isogenic line, Java
ANALISIS MOLEKULER PIRAMIDA GEN XA PADA PROGENI PADI VARIETAS CIHERANG DAN INPARI 13 ., Fatimah; Prasetiyono, Joko; Priyatno, Tri Puji; Yunus, Muhammad; Suhartini, Tintin; Ridwan, Iman; Baroya, Mushlihatun
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2161

Abstract

Bacterial leaf blight (BLB) is a major disease in Indonesian lowland rice.This research was undertaken to pyramid three BLB resistant genes xa5, Xa7 and Xa21 and one background BLB resistant gene Xa4 into Ciherang and Inpari 13 varieties. The donor parent Code (Xa4+Xa7) was crossed with Angke (Xa4+xa5) while Ciherang and Inpari 13 were crossed with IRBB21 (Xa21). Progenies were selected using marker assisted selection and yield component observation. Foreground selection was conducted using SSR and STS markers linked with the targeted genes in the F1 and DCF1 population. Individuals with triple positives Xa genes were screened for the presence of Xa4 gene as the background. Selected heterozygote plants in F1 Code x Angke, F1 Ciherang x IRBB21 and F1 Inpari 13 x IRBB21 were used to develop DCF1 population. Molecular analysis on DCF1 population through alleles of three BLB resistant genes xa5, Xa7 and Xa21 and one background BLB resistant gene Xa4 resulted 8 (2,6%) in DCF1 Ciherang and 13 (3,5%) in DCF1 Inpari 13. Yield component characters on F1 Code x Angke resulted significant in number of panicle. F1 Ciherang x IRBB21, F1 Inpari 13 x IRBB21 and DCF1 Ciherang resulted significant in weight of empty grain while DCF1 Inpari 13 resulted no significance in all of observed characters. Keywords: Rice, F1 Population, DCF1 Population, molecular marker, Xa gene 
ISOLATION AND DISEASE ASSESSMENT OF XANTHOMONAS ORYZAE PV. ORYZAE FROM JAVA ISLAND AND PATHOGENIC ASSAY ON NEAR ISOGENIC LINES WITH DIFFERENT RESISTANT GENES ., Fatimah; Priyatno, TP.; Fadlillah, SH.; ., Hermanto; Baroya, M.; ., Mahrup; ., Wawan; Sasongko, D.; Suryadi, Y.; Kadir, TS.
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.2104

Abstract

Bacterial leaf blight disease caused by Xanthomonas oryzae pv oryzae (Xoo) is one of the important bacterial diseases,which is very destructive to rice plant. In this study, seventy isolates of Xoo were collected from several regions inWest Java, Central Java, Yogyakarta and East Java provinces and ten isolates from ICABIOGRAD collection. Theaim of the study was to survey variation and distribution of the pathogen and to study the interactions between theisolates and near isogenic lines carrying specific genes for bacterial leaf blight resistance. Twenty Xoo isolates wererandomly chosen to assess the differential characteristics of ten near-isogenic rice lines in Indonesia. The resultsshowed that xa5 resistant gene was the highest effective against the majority of Xoo isolate, followed by Xa21, andXa7 combined with Xa4 as the background. These findings are useful for rice breeding programs in designing stablebroad-spectrum bacterial blight resistant rice cultivars.Key words: Xanthomonas oryzae, resistant gene, Near isogenic line, Java
Co-Authors . Ermanto Aznura, Naya Chisa Baroya, Mushlihatun Baroya, Mushlihatun D. Sasongko Dian, Agnes Fazura, Aghnina Harahap, Nisrina Adilah Iman Ridwan, Iman Irfansyah, Ahmad Joko Prasetiyono Kataren, Yogi Karmelta Khairani, Umi Lubis, Siti Zahra Khostamarul Aspia M. Baroya, M. Mahrup ., Mahrup Muhammad Yunus Ritonga, Suhairi Saifurrahman, Rizky SH. Fadlillah, SH. Tintin Suhartini TP. Priyatno, TP. Tri Puji Priyatno TS. Kadir, TS. Vera Yunita Wardana, Aji Wawan . Y. Suryadi, Y. Yahya, Harun