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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) BERITA BIOLOGI JURNAL BIOLOGI INDONESIA

Tintin Suhartini

Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar No. 3A, Bogor 16111 Telp. (0251) 8337975

Author-ID : 389397

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance

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Analisis Molekuler Piramida Gen Xa pada Progeni Padi Varietas Ciherang dan Inpari 13 ., Fatimah; Prasetiyono, Joko; Priyatno, Tri Puji; Yunus, Muhammad; Suhartini, Tintin; Ridwan, Iman; Baroya, Mushlihatun
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2161

Bacterial leaf blight (BLB) is a major disease in Indonesian lowland rice.This research was undertaken to pyramid three BLB resistant genes xa5, Xa7 and Xa21 and one background BLB resistant gene Xa4 into Ciherang and Inpari 13 varieties. The donor parent Code (Xa4+Xa7) was crossed with Angke (Xa4+xa5) while Ciherang and Inpari 13 were crossed with IRBB21 (Xa21). Progenies were selected using marker assisted selection and yield component observation. Foreground selection was conducted using SSR and STS markers linked with the targeted genes in the F1 and DCF1 population. Individuals with triple positives Xa genes were screened for the presence of Xa4 gene as the background. Selected heterozygote plants in F1 Code x Angke, F1 Ciherang x IRBB21 and F1 Inpari 13 x IRBB21 were used to develop DCF1 population. Molecular analysis on DCF1 population through alleles of three BLB resistant genes xa5, Xa7 and Xa21 and one background BLB resistant gene Xa4 resulted 8 (2,6%) in DCF1 Ciherang and 13 (3,5%) in DCF1 Inpari 13. Yield component characters on F1 Code x Angke resulted significant in number of panicle. F1 Ciherang x IRBB21, F1 Inpari 13 x IRBB21 and DCF1 Ciherang resulted significant in weight of empty grain while DCF1 Inpari 13 resulted no significance in all of observed characters.ÃÂ Keywords: Rice, F1 Population, DCF1 Population, molecular marker, Xa geneÃÂ

PERTUMBUHAN AKAR DUAPULUH GENOTIP PADI GOGO PADA KAHATFOSFOR DAN CEKAMAN ALUMINIUM Suhartini, Tintin
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Phosphorous insufficiency is a limiting factor for rice production. P deficiency in acid soil of tropical regions causing fixation of Al. Because of it, the giving P fertilizers in acid soil become not efficient. To exploit tolerance varieties to P deficiency and Al toxicity are one of the solutions to decrease P fertilizers. The objective of this experiment was to screen twenty upland rice genotypes for P deficiency and Al toxicity, and double stress Al toxicity and P deficiency in culture solution. The experiment was arranged in a split plot design with three replications. The main plots were aluminium treatment (without and 45 ppm Al), the sub plots were P treatments (0 ppm P, 0,5 ppm P, 5 and 10 ppm P) and twenty genotypes of upland rice were sub-sub plot. The root length and root dry weight characters were used to identify genotypes tolerant to P deficiency and Al toxicity. The results indicated that based on the root length character, Sentani and K36-5-1-1-1 were tolerant to Al toxicity and double stress P deficiency and Al toxicity; while based on the root dry weight character, K36-5-1 -1-1 and NIL-C443 were tolerant to P deficiency, Al toxicity and double stress P deficiency.

PENGELOMPOKAN PLASMANUTFAH SPESIES PADI LIAR {Oryza spp.) BERDAS ARKAN PEUB AH KUANTITATIF TANAMAN Suhartini, Tintin; Sutoro, Sutoro
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Wild rice species are important gene sources for the rice improvement programs.The morphological characters is the easiest way to identify the crop specificity, and it is applicable for determining the relation among species.The grouping on wild rice species are determined by using cluster analysis through the principal component analysis which involved 16 characters of quantitative traits of wild rice species. The total sample of wild rice species was 89 accessions of 18 species.The research was done at green house level during 3 seasons from 2004 to 2005.All the accessions of wild rice were planted in pots consists of 10 kg soil by 3 replications for each accession.The result of analysis of the first principal component from quantitative characters (plant height,amount of grain fill per panicle, amount of grain total per panicle, panicle length, 1000 grains weight, grain shape, days of flowering, total internodes and awning length ) could explain that variability were 82 %.The result of cluster analysis involved the four of principal component with similarity level equal to 80 %, obtained 5 groups of wild rice species. The result of the clusters was Oryza sativa included at cluster I, while O. officinalis had 3 clusters that is cluster III, IV and V. While O. meyeriana and Oryza ridleyi joint at cluster II.Quantitative character was applicable for clustering the accessions of wild rice species as according to each genome.

EVALUASI KARAKTER PEKA PANJANG HARI (PHOTOPERIOD) PADA TIGA GOLONGAN (Subspecies) PADI (Oryza sativa) SERTA PENGARUHNYA TERHADAP KARAKTER AGRONOMIS Suhartini, Tintin
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Day length or photoperiod is a situation of replacement of light period to dark which able to control the flower initiation and also growth of crop. Rice is short day plant; if day length exceed the critical phase it will be late of flowering.The purpose of this experiment was to evaluate rice cultivars which differ in groups and agronomic characters to photoperiod.The experiment was done at two seasons in November 1997 and May 1998 at green house BB-BIOGEN Bogor. The total of 29 cultivars consist of 3 subspesies of javanica, indica and japonica type were used. The experiment was based on randomized block design with 4 replications. The result showed the local varieties were sensitive and javanica were medium, while improved varieties and japonica type were insensitive to photoperiod. The effect of seasons were significantly different to flowering days of local and javanica types, significantly and not significantly different to japonica, while not significantly different to improved varieties. The season has not significant effect to panicle length and unfilled grain per panicle characters for all cultivars type. Plant heigth and dry straw per hill were higher in rainy season, while fill grain per panicle, total grain per panicle and 1000 grain weight were higher in dry season. The results showed there have correlation between flowering days to all characters were observed, except number of tiller in rainy season was not correlated.

ANALISIS MOLEKULER PIRAMIDA GEN XA PADA PROGENI PADI VARIETAS CIHERANG DAN INPARI 13 ., Fatimah; Prasetiyono, Joko; Priyatno, Tri Puji; Yunus, Muhammad; Suhartini, Tintin; Ridwan, Iman; Baroya, Mushlihatun
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2161

Bacterial leaf blight (BLB) is a major disease in Indonesian lowland rice.This research was undertaken to pyramid three BLB resistant genes xa5, Xa7 and Xa21 and one background BLB resistant gene Xa4 into Ciherang and Inpari 13 varieties. The donor parent Code (Xa4+Xa7) was crossed with Angke (Xa4+xa5) while Ciherang and Inpari 13 were crossed with IRBB21 (Xa21). Progenies were selected using marker assisted selection and yield component observation. Foreground selection was conducted using SSR and STS markers linked with the targeted genes in the F1 and DCF1 population. Individuals with triple positives Xa genes were screened for the presence of Xa4 gene as the background. Selected heterozygote plants in F1 Code x Angke, F1 Ciherang x IRBB21 and F1 Inpari 13 x IRBB21 were used to develop DCF1 population. Molecular analysis on DCF1 population through alleles of three BLB resistant genes xa5, Xa7 and Xa21 and one background BLB resistant gene Xa4 resulted 8 (2,6%) in DCF1 Ciherang and 13 (3,5%) in DCF1 Inpari 13. Yield component characters on F1 Code x Angke resulted significant in number of panicle. F1 Ciherang x IRBB21, F1 Inpari 13 x IRBB21 and DCF1 Ciherang resulted significant in weight of empty grain while DCF1 Inpari 13 resulted no significance in all of observed characters.Â Keywords: Rice, F1 Population, DCF1 Population, molecular marker, Xa geneÂ

Peranan Plasma Nutfah Padi dalam Meningkatkan Produktivitas Lahan Keracunan Besi Suhartini, Tintin
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 29 No. 2 (2001): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Productivity of land in Java insel decreased. Therefore, it is necessary to utilize marginal Fe toxic land in order to increase production of rice. One of the hinderness is the lack of rice variety which is tolerant to this condition. The rice research institute and Balitbio in Bogor have the collection of rice varieties which can be used in breeding program. The program obtained a rice variety that is adaptive to Fe toxic land. Key words: Rice, Iron

Evaluation of Several Pup1-Lines of Rice (Oryza sativa L.) on Nutrient Solution and at the Field Prasetiyono, Joko; Suhartini, Tintin; Soemantri, Ida Hanarida; Tasliah, ,; Moeljopawiro, Sugiono; Aswidinnoor, Hajrial; Sopandie, Didy; Bustamam, Masdiar
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 40 No. 2 (2012): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Phosphorus (P) deficiency is one of major problems in rice cultivation. Development of a tolerant variety to P deficient soil is expected to reduce the needs of P fertilizer. The aim of this research was to evaluate the agronomic performance of Pup1-introgression rice lines. This research consisted of two separate experiments, an evaluation on rice grown in nutrient solution in the greenhouse of ICABIOGRAD, Bogor; and field evaluation at upland rice screening site Taman Bogo, Lampung. The rice lines used were BC2 F3 derived from crossing of Situ Bagendit with Kasalath and NIL-C443, and from crossing of Batur with Kasalath and NIL-C443. Molecular analysis was conducted to verifiy the Pup1 introgression in the lines used and successfully confirmed the occurance of homozygote Pup1 segment in those lines. Evaluation of rice lines grown in nutrient solution was performed in a split plot factorial design, using the dose of Al (0 and 45 ppm Al3+) as main plots and dose of P (0.5 and 10 ppm P) as subplots. Field evaluation was performed in split plot design, with P fertilization (0 and 500 kg SP-18 ha-1) as main plots and BC2 F3 lines as the subplots. Evaluation using Yoshida nutrient solution showed that the BC2 F3 lines had greater total dry weight under low-P condition (37.5-112.5%), especially under Al-toxicity, compared to the respective parental varieties (Situ Bagendit and Batur). Field evaluation showed that the Pup1-introgression lines had greater shoot dry weight than the respective parental lines (10.5-74.82%). However there was no significnant effect of Pup1 introgression in terms of weight of filled grain.

Co-Authors , Tasliah Baroya, Mushlihatun Baroya, Mushlihatun Didy Sopandie Fatimah . HAJRIAL ASWIDINNOOR Ida Hanarida Soemantri Iman Ridwan, Iman Joko Prasetiyono Masdiar Bustamam Muhammad Yunus SUGIONO MOELJOPAWIRO Sutoro Sutoro Tri Puji Priyatno

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