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All Journal Rumphius Pattimura Biological Journal
Moniharapon, Debby
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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THE EFFECT OF LEMONGRASS EXTRACT (Cymbopogon citratus L.) ON PEST MORTALITY OF Plutella xylostella L. IN MUSTARD PLANTS (Brassica juncea L.) Moniharapon, Debby; Nindatu, Maria; Bastian, Alien
RUMPHIUS Vol 3 No 2 (2021): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv3i2p034-038

Abstract

Cabbage leaf caterpillars (Plutella xylostella L., Lepidoptera: Plutellidae) are the main pests that are very damaging to Brassicaceae plants, especially cabbage, mustard greens, and caisin in Indonesia. The chemical content of lemongrass is citral, citronella, geraniol, mirsene, nerol, farnesol methil heptenol and dipentene. The purpose of this study was to determine the effective concentration of Plutella xylostella pest mortality and the right LC50 value of Plutella xylostella pest mortality. This research is experimental using a completely randomized design. The subjects were 50 plutella xylostella L. Instar III pests. The concentrations used were 10 g, 20 g, 30 g, 40 g with negative control 0 g (aquades) with 5 repetitions for each treatment group. Observations were made 24 hours after spraying and the results obtained were an increase in pest death with increasing concentration. Based on the results of analysis of variance seen in the calculated F value (41,962)> F table (2,866). This proves that the administration of lemongrass stem extract significantly influences the mortality of Plutella xylostella in mustard plants (Brassisca juncea). The results of LC50 probit analysis were obtained at the concentration of lemongrass extract at 21,277% with a lower limit of 17,782 and an upper limit of 24,824, meaning that at a concentration of 21,277% lemongrass extract was able to kill 50% of the Plutella xylostella pest used after 24 hours of administration at a 95% confidence level.
ACTIVITY OF CLOVE LEAVES (Syzygium aromaticum L.) LARVACIDES ON MORTALITY OF Anopheles sp. Sapulette, Fenska Violenta; Unitly, Adrien Jems Akiles; Moniharapon, Debby
RUMPHIUS Vol 1 No 2 (2019): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv1i2p042-046

Abstract

This study used a completely randomized design with a sample of 525 Anopheles sp. larvae. which were divided into 7 groups and 3 replicates, which contained 25 Anopheles spper larvae. Except for the control, each group was given clove leaf infusion starting from a concentration of 0.50%, 1%, 2%, 4% and 6%, where the treatment was carried out for 24 hours. The results showed that there was a larvicidal effect of brewing clove leaves (Syzygium aromaticum L.) on the mortality of Anopheles sp mosquito larvae, where the effective concentration was 6% and had an LC50 value in killing Anopheles sp larvae of 0.89%. The overall phytochemical content of clove leaves which has a fast response to the mortality of Anopheles sp. are saponins, flavonoids and tannins which are capable of acting as larvicides through the mechanism of damaging cell membranes or interfering with larval metabolic processes. The content of saponins, flavonoids and tannins can increase the mortality of Aedes aegypti L. larvae through the mechanism of damaging cell membranes or interfering with larval metabolic processes as stomach poisoning.
Co-Authors Adrien Jems Akiles Unitly Bastian, Alien Nindatu, Maria Sapulette, Fenska Violenta