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Fakultas Peternakan UGM
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Follicle development and FSH secretion pattern of Ongole crossbred cow with natural twin birth history ., Aryogi; E, Baliarti; ., Sumadi; ., Kustono
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 3 (2013)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (807.486 KB) | DOI: 10.14334/jitv.v18i3.318

Abstract

A research was done to obtain basic data about influence of natural twin birth history on reproduction of Ongole crossbred (PO) cow, as a basic consideration in utilizing twin genetic potency to increase beef production. The research was done for three estrus cycles respectively, and consists of two activities. The first activity was done in Beef Cattle Research Station (BCReS) at Grati sub-district, Pasuruan district – East Java, to observe number and development of follicle of ten cows with natural twin birth history (TP) and ten cows with single birth history (SP). In this activit ultra sonography (USG) equipment was used. The second activity was done in BCReS and in the Faculty of Veterinary Airlangga University at Surabaya, to observe concentration and profile of FSH using Bovine Blood Serum kit and IRMA method of five cows with TP and five PO cows with SP. Data obtained were analyzed using Chi-square test, t-test and descriptive presentation. Result shows that 23.33% of cows with TP produced two dominant (de Graf) follicles in an estrus cycle and significantly (P < 0.01) higher FSH secretion concentration (1.26 – 3.13 times) than that of cows with SP. It is concluded that PO cows with TP can produces more than one de Graf follicles in an estrus cycle and it has very high secretion concentration level of FSH. Key Words: Twin Births, PO Cow, Follicle, FSH
Morphological diversity and genetic differentiation of PO cattle in smallholder farmers Hartatik, Tety; ., Hartati; ., Sumadi; ., Subandriyo
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 1 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.518 KB) | DOI: 10.14334/jitv.v15i1.680

Abstract

PO cattle is one of  the local cattle with high genetic diversity. The aim of this research was to study genetic diversity of PO cattle in smallholder farmer  based on morfology and molecular markers. This research was conducted at breeding center in East Java and Central Java include Tuban, Lamongan and Blora regency, since June until December 2008.  PO bull used were of 18 months until 24 months of age and cow of 24 months until 36 months of age or at first calving as much as 30 head each location. The variables measured were body weight, body length, withers height, hip height, shoulder point width, chest girth, chest depth, canon bone circumference, head length and head width. Data were analyzed using descriptive statistic, average analysis between subpopulations, discriminant and canonical analysis and mahalanobis distance was done by SAS DISCRIM procedure. Whole blood were collected to get the data of polymorphism DNA microsatellite using technology of PCR and electrophoresis using 6 microsatellite loci. Variable measured were number and size of  alel, alel frequency, heterozygosity,genetic distance and fixation index (F-stat). Data were analyzed using analysis of Microsate Toolkit, GENEPOPV4, MSA (Microsatellite Analyser) and Mega 4. The result showed that Blora and Tuban subpopulation have genetic diversity which is relative lower  compared to that of Lamongan. The genetic distance which was close presented by Tuban and Blora subpopulation while Lamongan and Tuban subpopulation show far genetic distance. The result of canonical analyses showed  high correlation on shoulder point width, head width, body length, withers height and hip height variable so this five variable can be used as distinguishing variables among subpopulation. The result of molecular genetics analysis using microsatellite showed that highest frequency of alel presented by HEL9 locus at Tuban population so this loci have the high polymorfism. The genetic differentiation among subpopulation showed by differentiation FST value among six loci  indicating the increasing of inbreeding in  the three subpopulations. Key words: Morphological Diversity, Genetic Differentiation, PO Cattle
Reproductive performances of Boe, Kacang and Boerka does Elieser, Simon; ., Sumadi; Suparta, G; ., Subandriyo
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 2 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.639 KB) | DOI: 10.14334/jitv.v17i2.683

Abstract

Does reproduction performance could be expressed by her ability to give birth and to give milk to their kids during the pre-weaning period. This study was aimed to evaluate the reproductive performance of Boer, Kacang and Boerka does; and was carried out for two years at Research Institute for Goat Production, Sungei Putih. The materials used were goats owned by the institute. The parameters observed were: litter size, parity of does, preweaning mortality, kidding interval and sex ratio of kids. The rate of reproduction of the does was estimated using Amir and Knipscheer methods and were statistically analyzed using General linear model. Results showed that litter size and kidding interval of Boer goats were higher (P < 0.05) then that of Kacang goats, while the Boerka goats was in between. The mortality at preweaning of Boer goats was lower (P < 0.05) then that of Kacang goats, while the Boerka goats was in between. The percentage of kid sex ratio of three breeds were fluctuated. Parity of does had significant effect on all reproduction traits (P < 0.05) except for sex ratio of kids. The does reproduction was smallest at the first parity, and increased with the increase of parity from one to four, and then decreased in subsequent parities. The highest does reproduction rate was found in the Boerka (1.82), followed by Boer (1.80) and Kacang (1.80). It is concluded that the reproductive performance of the three breed female goat was relatively the same. Key Words: Performance, Reproductive, Boer, Kacang
The Application of Polymerase Chain Reaction – Restriction Fragment Polymorphisms (PCR-RFLP) to Determine Genetic Diversity of Madura Cattle in Sapudi Island Hartatik, Tety; Volkandari, Slamet Diah; ., Sumadi; ., Widodo
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.889 KB)

Abstract

The aim of this study was to determine genetic diversity of Madura cattle using Polymerase Chain Reaction – Restriction Fragment Length Polymorphisms (PCR-RFLP) analysis of the cytochrome b (cytb) gene. Samples used for the experiments were blood of 43 cattle that consist of 15 cattle obtained from Madura Island, 23 cattle from Sapudi Island, and 5 Limousin-Madura (Limura) cattle. A fragment of 464 base pair of cytb gene was amplifi ed by forward primer L14735 and reverse primer H15149. The PCR product was digested with TaqIand HinfI restriction enzymes to identify genetic patterns. Data of PCR-RFLP showed two haplotypes, that were A and B, in cattle obtained from both Madura Island and Sapudi Island. The frequencies of haplotype A and B of cattle from Sapudi Island were 69.57% and 30.47%, respectively. More diverse frequencies were observed in cattle obtained from Madura Island, where haplotype A and B were 86.67% and 13.33%, respectively. In this experiment, Limura cattle had only haplotype A. As a conclusion, PCR-RFLP of the cytb gene had been able to determine a genetic diversity of Madura cattle.Key words: Genetic diversity, Madura cattle, haplotype.
Co-Authors Aryogi . Baliarti E G Suparta Hartati . Kustono . Simon Elieser Slamet Diah Volkandari, Slamet Diah Subandriyo . Tety Hartatik