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All Journal Jurnal Ilmu Dasar Indonesian Journal of Biotechnology JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pendidikan IPA (JPPIPA) Makara Journal of Science
Slamet Diah Volkandari, Slamet Diah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Education Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics

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The Application of Polymerase Chain Reaction – Restriction Fragment Polymorphisms (PCR-RFLP) to Determine Genetic Diversity of Madura Cattle in Sapudi Island Hartatik, Tety; Volkandari, Slamet Diah; ., Sumadi; ., Widodo
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.889 KB)

Abstract

The aim of this study was to determine genetic diversity of Madura cattle using Polymerase Chain Reaction – Restriction Fragment Length Polymorphisms (PCR-RFLP) analysis of the cytochrome b (cytb) gene. Samples used for the experiments were blood of 43 cattle that consist of 15 cattle obtained from Madura Island, 23 cattle from Sapudi Island, and 5 Limousin-Madura (Limura) cattle. A fragment of 464 base pair of cytb gene was amplifi ed by forward primer L14735 and reverse primer H15149. The PCR product was digested with TaqIand HinfI restriction enzymes to identify genetic patterns. Data of PCR-RFLP showed two haplotypes, that were A and B, in cattle obtained from both Madura Island and Sapudi Island. The frequencies of haplotype A and B of cattle from Sapudi Island were 69.57% and 30.47%, respectively. More diverse frequencies were observed in cattle obtained from Madura Island, where haplotype A and B were 86.67% and 13.33%, respectively. In this experiment, Limura cattle had only haplotype A. As a conclusion, PCR-RFLP of the cytb gene had been able to determine a genetic diversity of Madura cattle.Key words: Genetic diversity, Madura cattle, haplotype.
DIVERSITY OF FRUIT FLIES (DIPTERA: TEPHRITIDAE) ATTRACTED BY ME LURE IN CSC-BG GERMPLASM CARAMBOLA PLANTATION Riastiwi, Indira; Paradisa, Yashanti Berlinda; Mambrasar, Yasper Michael; Raunsai, Marlin Megalestin; Perwitasari, Urip; Volkandari, Slamet Diah; Sari, Nurul Fitri; Sulistiyani, Tri Ratna; Ibo, Leberina Kristina
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 2 (2021): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.221151-157

Abstract

Diversity of fruit flies (Diptera: tephritidae) attracted by me lure in CSG-BG germplasm carambolae plantation. Germplasm Garden (KPN) Cibinong Science Center-Botanical Garden (CSC-BG) is one of the gardens that conserve and utilizes Indonesian local germplasm, one of which is carambolae. The current problem in KPN is the attack of fruit flies (Bactrocera spp.). This study was conducted to determine the diversity of fruit flies in the KPN carambola plantation area. A total of eight traps were placed randomly in the plantation area. Each trap contained 1 mL of methyl eugenol pheromone solution. Identification of fruit flies was carried out based on three morphological differences, they were wings, abdomen, and hind legs. There were 317 fruit flies collected and separated into three species, namely Bactrocera carambolae, B. papayae, dan B. umbrosa, of which B. carambolae had a dominance index of 0.74 and abundance index of 85%. Data on the types of fruit flies can be used as primary data to determine the preparation of pest prevention efforts.
Confirmation of Existing Insulin-like Growth Factor-1 Gene Associated with Growth and Milk-Production Traits and Genetic Diversity in Buffalo Margawati, Endang Tri; Volkandari, Slamet Diah; Indriawati,; Thalib, Chalid
Makara Journal of Science Vol. 21, No. 3
Publisher : UI Scholars Hub

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Insulin-like growth factor-1 (IGF-1) gene plays an important role in the endocrine system of animals by regulating nutrient metabolism, growth, and milk production. There have been extensive molecular genetics research studies on cattle but less studies have focused on buffalo (Bubalus bubalis). This study aimed to confirm the association of IGF-1 gene in swamp or river buffalo (B. bubalis spp.) with growth and milk production traits. DNA samples were obtained from 12 buffalos (eight swamp buffalo and four river buffalo). One Bali cattle (Bos javanicus) was included as an outgroup (control). The eight swamp buffalo originated from East Nusa Tenggara (n = 1), Baluran, East Java (n = 4), and Banyuwangi, East Java (n = 3), while the four river buffalo originated from Sei Putih, Medan of North Sumatera. All DNA samples were amplified using an IGF-1 primer for 30 cycles, and amplicons were visualized on 1% agarose gel. Five of the 13 samples were sequenced to determine nucleotide sequence variation between the swamp and river buffalo. The results revealed that the size (225–231 bp) of all the fragments was in in accordance with that of IGF-1. There was not found genetic variation among the buffalo samples. The results indicate that buffalo samples bear growth and milk production traits.
The Application of UTY and SRY Molecular Markers for Determination of Unknown Sex Samples in Bali Cattle Indriawati, Indriawati; Volkandari, Slamet Diah; Margawati, Endang Tri
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.781 KB) | DOI: 10.19184/jid.v21i1.9333

Abstract

An investigation involving large number of animals is often resulting incomplete or in accurate information such as animal parentage, or misidentify on sex due to unlabeled sex samples. A PCR method by applying Y chromosome markers (UTY and SRY) facilitates in determination of unknown sex problem. This study was intended to determine sex from unlabelled sex of blood samples by applying PCR method using a pooled-DNA template. Twenty five of unknown sex blood samples from Nusa Penida, Bali were used in this study. The samples were plotted into 5 pooled-DNA whith each pool DNA consisted of 5 individuals DNA. Two pairs of sex primers, UTY (58oC) and SRY (60oC) with 35 cycles were applied to amplify the samples. The result showed there was only one pooled-DNA (P4) amplified by UTY (484bp). Whereas re-PCR of the positive pooled-DNA (P4) using SRY primer, only one out of 25 samples determined as male Bali cattle (325bp). This finding suggests that UTY and SRY primers are suitable for sex determination and the pooled-DNA could be used as an efficient PCR method both in consumables and PCR process for sex determination. Keywords: Determination, sex, unknown sample, pooled DNA, Bali cattle.
Impact of Environmental Geographic toward Point Mutations in Exon 1 of Growth Differentiation Factor (GDF9) Gene in Kosta and Lakor Goat Breeds Rumanta, Maman; Kunda, Rony Marsyal; Volkandari, Slamet Diah; Munir, Ivan Mambaul
Jurnal Penelitian Pendidikan IPA Vol 9 No 6 (2023): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i6.3794

Abstract

This study aims to analyze the impact of environmental biogeography on point mutations in Kosta and Lakor goat breeds as local Indonesian germplasm using the GDF9 Exon 1 gene sequence. A total 43 folicle samples of Kosta and Lakor goats which collected in Serang regency and Lakor island were analyzed. To isolate the total genome of the samples, the tissue isolation method was used. The polymerase chain reaction (PCR) method was used for amplification of the exon-1 region of the GDF9 gene with two pairs of primers. Analysis of genetic variation and single nucleotide polymorphisms (SNP) using MEGA software version X. Multiple alignment results showed that there are 2 nucleotides that have mutations, but not encode different amino acids. This results indicates that the mutation has association with litter size of two breeds. This finding shows that Lakor goats have polymorphism i.e SNP g.54C>T which is significantly associated with litter size. The analysis of GDF9 gene showed that all samples from the Two local Indonesian goat breeds (Kosta and Lakor) are  monomorphic. But, although both are monomorphic, geographical isolation still contributes to genetic drift within and between populations.
Co-Authors Chalid Thalib ENDANG TRI MARGAWATI Ibo, Leberina Kristina Indriawati, Indriawati, Indriawati Maman Rumanta Munir, Ivan Mambaul Paradisa, Yashanti Berlinda Raunsai, Marlin Megalestin Riastiwi, Indira Rony Marsyal Kunda Sari, Nurul Fitri Sumadi . Tety Hartatik TRI RATNA SULISTIYANI Urip Perwitasari, Urip Yasper Michael Mambrasar, Yasper Michael