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All Journal Jurnal Pasca Sarjana : EDUKASI Nexus Pendidikan Kedokteran dan Kesehatan Nexus Biomedika Nexus Kedokteran Translasional
Suyatmi .
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Biochemistry, Genetics & Molecular Biology Education Health Professions Medicine & Pharmacology

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Journal : Nexus Biomedika

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Pengaruh Ekstrak Buah Jambu Biji Merah terhadap Kerusakan Struktur Histologis Hepar Mencit yang Diinduksi Asetaminofen Wardana, Vasa Adi Wisnu; ., Suyatmi; Sakiman, Bambang Sukilarso
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Antioxidants in guava fruit are vitamin C, flavanoid, and lycopene which are estimated having hepato-protective function. The objectives of this research are to know the effect of guava fruit extract and in various doses on mice liver damage induced by acetaminophen. Methods: Samples of 28 male mice, Swiss webster type, 2-3 months old age and + 20gr of each weight, were divided into 4 groups of 7 mice. Guava fruit extract was given for 14 days in a row, while acetaminophen in toxic dose was given on the 12th, 13th, and 14th day. The score of hepatocyte damage was determined by pyknosis, karyorhexis, and karyolysis. The data was analized by One-Way ANOVA test (α= 0,05) and continued by Post Hoc Multiple Comparisons using Least Significant Differences(LSD) test(α= 0,05). Results:The group with the highest average damage of hepatocyte is KP1(84,23±3,207), and the groups with decrease average damage of hepatocyte are KP2 (48,76±2,964) and KP3(35,23±2,755). The results of One-Way ANOVA test showed a significant difference in all group (p<0.001). The statistical data result showed a significant difference of hepatic damage between K-P1, K-KP2, K-KP3, KP1-KP2, KP1-KP3, and KP2-KP3. Conclusion: Guava fruit extract can decrease the histological damage of the hepatocyte of mice induced by acetaminophen. The higher dose of guava fruit extract, the higher protection effect againts liver histological damage from acetaminophen toxic on mice. Key words: guava fruit extract, acetaminophen, histological damage, hepatocyte.
Pengaruh Ekstrak Buah Jambu Biji Merah terhadap Kerusakan Struktur Histologis Hepar Mencit yang Diinduksi Asetaminofen Vasa Adi Wisnu Wardana; Suyatmi .; Bambang Sukilarso Sakiman
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.86 KB)

Abstract

Background: Antioxidants in guava fruit are vitamin C, flavanoid, and lycopene which are estimated having hepato-protective function. The objectives of this research are to know the effect of guava fruit extract and in various doses on mice liver damage induced by acetaminophen. Methods: Samples of 28 male mice, Swiss webster type, 2-3 months old age and + 20gr of each weight, were divided into 4 groups of 7 mice. Guava fruit extract was given for 14 days in a row, while acetaminophen in toxic dose was given on the 12th, 13th, and 14th day. The score of hepatocyte damage was determined by pyknosis, karyorhexis, and karyolysis. The data was analized by One-Way ANOVA test (?= 0,05) and continued by Post Hoc Multiple Comparisons using Least Significant Differences(LSD) test(?= 0,05). Results:The group with the highest average damage of hepatocyte is KP1(84,233,207), and the groups with decrease average damage of hepatocyte are KP2 (48,762,964) and KP3(35,232,755). The results of One-Way ANOVA test showed a significant difference in all group (p<0.001). The statistical data result showed a significant difference of hepatic damage between K-P1, K-KP2, K-KP3, KP1-KP2, KP1-KP3, and KP2-KP3. Conclusion: Guava fruit extract can decrease the histological damage of the hepatocyte of mice induced by acetaminophen. The higher dose of guava fruit extract, the higher protection effect againts liver histological damage from acetaminophen toxic on mice. Key words: guava fruit extract, acetaminophen, histological damage, hepatocyte.
Hepatoprotective Effect of Ethanolic Extract of Pegagan Leaves (Centella asiatica L. Urban) on Histological Damage of Hepatocyte on Mice Model induced by Parasetamol Naila Shofwati Putri; Suyatmi .; Ratih Puspita Febrinasari
Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Pegagan leaves (Centella asiatica L. Urban) contain antioxidative compounds that can protect hepatocytes from free radicals. The aim of this research was to know the hepatoprotective effect of ethanolic extract of pegagan leaves (Centella asiatica L. Urban) on histological damage of hepatocyte on mice model induced by paracetamol. Methods: This study was an experimental laboratory with the post test only control group design. Samples were 30 male Swiss webster mice, with 2-3 months old and 20 gr body weight divided into 5 groups equally. KN and KI were given distilled water for 14 days. In addition, KI was also given paracetamol in dose 5.07 mg/20 gbw on the 12th, 13th, 14th day. KP1, KP2, and KP3 were given ethanolic extract of pegagan leaves in dose 5 mg, 10 mg, and 20 mg per 20 gbw respectively for 14 days and paracetamol in dose 5.07 mg per 20 gbw on the 12th, 13th, 14th day. On the 15th day, mice were sacrificed and their livers were taken for preparation with paraffin block method and stained with HE. The features of histological damage of hepatocyte were assessed by counting the numbers of nucleus damage of hepotocyte (pyknosis, karyorrhexis, karyolysis) of 100 cells in the sentrolobular zone. The data were analyzed by One-way ANOVA test and Post Hoc LSD test. \ Results: The means of histological damage of hepatocyte were 16.08 5.160; 66.00 3.275; 44.58 5.583; 31.67 2.188; 26.17 3.810 for KN, KI, KP1, KP2, KP3 respectively. The result of One-Way ANOVA test was *p = 0.000. The result of Post Hoc LSD test showed a significant difference between five groups, *p = 0.000 for KN-KI, KN-KP1, KN-KP2, KN-KP3, KI-KP1, KI-KP2, KI-KP3, KP1-KP2, KP1-KP3 and *p = 0.002 for KP2-KP3. Conclusion: The ethanolic extract of pegagan leaves can prevent histological damage of hepatocyte on mice model induced by paracetamol. Keywords: ethanolic extract of pegagan leaves, paracetamol, histological damage of hepatocyte
Bilimbi Fruit (Averrhoa bilimbi Linn.) Extract Protects Liver from Damage Induced by Reheated Palm Oil on Mice (Mus musculus) Muhammad Hilmy Labibi; Suyatmi .; Zulaika Nur Afifah
Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Introduction: Liver diseases such as hepatitis, cirrhosis hepatis, and fatty liver disease are serious health problem worldwide. Stress oxidative is involved in the pathogenesis of the diseases. Bilimbi fruit (Averrhoa bilimbi Linn.) contains flavonoid, triterpenoid saponin, vitamin A, and vitamin C which might protect the liver from stress oxidative. This research aimed to know the effect of Averrhoa bilimbi Linn. extract on the liver damage induced by reheated palm oil on mice. Methods: This was an experimental laboratory research with the post test only controlled group design. This research used 30 male Swiss webster mice aged 2-3 months and weighed 20 g weight divided randomly and equally into 5 groups. The normal control group (KKn) was given distilled water, the exposure group (KKp) was given reheated palm oil, and the 1st,2nd3rd treatment group (KP1, KP2, KP3) were given ethanolic extract of Averrhoa bilimbi Linn. in dose 5.6 mg, 11.2 mg, and 22.4 mg respectively. The induction for liver damage was done by giving reheated palm oil afterward. These treatments were given for 14 days. Mice were sacrificed on the 15th day by neck dislocation. The liver were obtained for preparation with paraffin block method and stained with HE. The Liver damage was assessed by quantifying of necrotic cells of each 100 cells in the centrolobular zone. Data was analyzed using One-Way ANOVA test and followed by Post Hoc test (?=0,05). Results: Mean of necrotic cells for KKn, KKp, KP1, KP2, and KP3 were 5.171.53; 78.084.74; 40.336.34; 30.176.29; and 19.005.89 cells respectively. One-Way ANOVA test showed a significant differences of liver damage score among five groups, *p=0.000. The results of Post Hoc test also showed significant difference between pairs of all group (*p =0.000). Conclusion: Averrhoa bilimbi Linn.extract protected liver from damage induced by reheated palm oil on mice. Keywords: Averrhoa bilimbi Linn. extract, reheated palm oil, liver histological damage
Co-Authors Bambang Sukilarso Sakiman Bambang Sukilarso Sakiman Dewantari Saputri Kusmadewi Eka Damayanti Leli Saptawati Muhammad Afif Muhammad Hilmy Labibi Naila Shofwati Putri Ratih Puspita Febrinasari Sheilla Elfira San Pambayun Vasa Adi Wisnu Wardana