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Hubungan Lama Penggunaan Kontrasepsi Oral Kombinasi dengan Tekanan Darah pada Wanita Usia Subur Febriniata, Mustiqa; ., Widardo; Febrinasari, Ratih Puspita
Nexus Kedokteran Komunitas Vol 1, No 2 (2012): Nexus Kedokteran Komunitas
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Combined oral contraception is one of contraception methods that contains estrogen and progesterone. Both of that hormones have an effect on blood pressure levels because of the using duration of combined oral contraception, Body Mass Index (BMI), and acceptor age. The purpose of this research is to analyze the correlation between the using duration of combined oral contraception and blood pressure levels among fertile women in Jaten area, Karanganyar. Methods: This research used observational cross sectional analytic approach method. The population were fertile women who use combined oral contraception. The total sample were 48 patients who appropriate with inclusion and exclusion criterias. The data were collected by kuesioner, interview, anthropometry measurement, and blood pressure direct measurement. Those variables such as the using duration of combined oral contraception, blood pressure levels (systolic and diastolic), age, and body mass index (BMI) were analyzed using Kolmogorof-Smirnov’s test of normality and linear regression analysis using SPSS 16 for Windows. Results: The result of simple statistic test using linear regression showed the correlation significancy between the using duration of combined oral contraception and blood pressure levels, both systolic (p=0,004) and diastolic (p=0,012). Conclusions: There is a correlation between the using duration of combined oral contraception and blood pressure levels among fertile women. Keywords: The using duration of combined oral contraception, Blood Pressure, Age, Body mass index  
Uji Sitotoksik Ekstrak Daun Andrographis paniculata Ness. pada Kultur Limfosit T Pasien Systemic Lupus Erythematosus Febriandaru, Dedi; Febrinasari, Ratih Puspita; Febrinasari, Ratih Puspita; Wijayanti, Lilik; Wijayanti, Lilik
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Systemic Lupus Erythematosus or SLE is a chronic autoimmune disease with varied clinical manifestations. SLE prevalence in Indonesia is being dominated with female, especially in their productive age. Certain etiology of SLE is still unknown until now, but genetic factors and immune system are being estimated as the primary factors. The first line therapy of SLE is cyclophosphamide. Unfortunately if being consumed more than six months, cyclophosphamide has several side effects, like bone marrow depression, infections, alopecia, hemorrhagic cystitis, and infertility. Because of that side effects, herbal medicine is being developed nowday. One of them is Andrographis paniculata Ness. leaf. The aim of this study was quantitative cytotoxic test for Andrographis paniculata Ness. leaf on T lymphocyte culture of Systemic Lupus Erythematosus. Methods: This study was anexperimental study. Subjects in this study were patients with SLE who appropriated SLE’s criterias and normal people without allergy and autoimmune diseases’s history. This study was done by doing T lymphocyte culture from intravenous’s blood sample and observing cell’s absorbance with ELISA reader. Collected data were used for determining live cell’s percentage (IC50) and analyzed with Statistical Product and Service Solution (SPSS) 11.00 for Windows. Mean resistance data was tested by linear regression test. Results:The result showed that IC50 dose of Andrographis paniculata Ness. leaf extract treatment on SLE samples was smaller than the normal people. In Andrographis paniculata Ness. leaf’s extract treatment, IC50 in SLE = 0.00074 and in normal people = 0.19584. Conclusion: Andrographis paniculata Ness.leaf extract had stronger cytotoxic effect on T lymphocyte culture of patients with SLE than the normal people. Keywords: Systemic lupus erythematosus, Andrographis paniculata Ness. leaf extract, T lymphocytes, IC50
Uji Sitotoksik Ekstrak Daun Andrographis paniculata Ness. pada Kultur Limfosit T Pasien Systemic Lupus Erythematosus Febriandaru, Dedi; Febrinasari, Ratih Puspita; Febrinasari, Ratih Puspita; Wijayanti, Lilik; Wijayanti, Lilik
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Systemic Lupus Erythematosus or SLE is a chronic autoimmune disease with varied clinical manifestations. SLE prevalence in Indonesia is being dominated with female, especially in their productive age. Certain etiology of SLE is still unknown until now, but genetic factors and immune system are being estimated as the primary factors. The first line therapy of SLE is cyclophosphamide. Unfortunately if being consumed more than six months, cyclophosphamide has several side effects, like bone marrow depression, infections, alopecia, hemorrhagic cystitis, and infertility. Because of that side effects, herbal medicine is being developed nowday. One of them is Andrographis paniculata Ness. leaf. The aim of this study was quantitative cytotoxic test for Andrographis paniculata Ness. leaf on T lymphocyte culture of Systemic Lupus Erythematosus. Methods: This study was anexperimental study. Subjects in this study were patients with SLE who appropriated SLE’s criterias and normal people without allergy and autoimmune diseases’s history. This study was done by doing T lymphocyte culture from intravenous’s blood sample and observing cell’s absorbance with ELISA reader. Collected data were used for determining live cell’s percentage (IC50) and analyzed with Statistical Product and Service Solution (SPSS) 11.00 for Windows. Mean resistance data was tested by linear regression test. Results:The result showed that IC50 dose of Andrographis paniculata Ness. leaf extract treatment on SLE samples was smaller than the normal people. In Andrographis paniculata Ness. leaf’s extract treatment, IC50 in SLE = 0.00074 and in normal people = 0.19584. Conclusion: Andrographis paniculata Ness.leaf extract had stronger cytotoxic effect on T lymphocyte culture of patients with SLE than the normal people. Keywords: Systemic lupus erythematosus, Andrographis paniculata Ness. leaf extract, T lymphocytes, IC50
Uji Sitotoksik Ekstrak Curcumin Pada Kultur Sel Limfosit T Penderita Systemic Lupus Erythematosus (SLE) Mustikasari, Hanif; Febrinasari, Ratih Puspita; Widardo, .
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Systemic lupus erythematosus (SLE) is an autoimmune disease that causes chronic inflammation in multiorgan, in which its etiology and pathogenesis are unclear. Curcumin has been found to have a selective cytotoxic effects on various types of cancer cells through induction of apoptosis on the various pathways. This study aimed to demonstrate whether curcumin extract had cytotoxic effects on T lymphocytes autoreactivity of patients with SLE. Methods: This research was an experimental laboratory with post test only controlled group design. Samples were using cell cultures of T lymphocytes from the veins blood of a patient with SLE and normal people. Each of T lymphocytes cell culture consisted of media control (KM), cells control (KS), the positive control with cyclophosphamide (KP1), and a group treated with curcumin extract (KP2). Curcumin extracts and cyclophosphamide were divided into 8 serial doses (50 µg/mL, 25 µg/mL, 12.5 µg/mL, 6.25 µg/mL, 3.125 µg/mL, 1.5625 µg/mL, 0.78125 µg/mL, 0.390625 µg/mL). Cell inhibition was calculated based on a percentage (%) of cell viability. Then, the data was analyzed using probit analysis to determine the dose of IC50 with SPSS for Window Release 17.0. Results: The result of cell inhibition probit analysis (IC50) on T lymphocytes cell culture of patients with SLE using curcumin extract was active (score 3) and T lymphocytes cell culture of patients with SLE using cyclophosphamide was moderately active (score 2).While, T lymphocytes cell culture of normal people using curcumin extract was active (score 3) and T lymphocytes cell culture of normal people using cyclophosphamide was moderately active (score 2). Conclusions: Curcumin extract had cytotoxic effect on T lymphocytes cell culture of SLE. The cytotoxic effect of curcumin extract on T lymphocytes cell culture of patients with SLE was stronger than cyclophosphamide.   Keywords: curcumin extract, cytotoxic, SLE
Perbedaan Efek Ekstrak Etanol Stevia Dibandingkan Madu Terhadap Perubahan Glukosa Darah Tikus Wistar Model Diabetik Fatimah R, Siti; ., Kisrini; Puspita Febrinasari, Ratih
Nexus Kedokteran Translasional Vol 1, No 1 (2012): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background:Stevia’s leaf contains steviol, stevioside and rebaudioside which have effect to increase secretion insulin. Honey, natural sweetener contains several antioxidant such as propolis, flavonoid and vitamin. It will eliminate free radicals and indirectly improve pankreas glands cells to produce insulin. This research was aimed to know the difference effect of stevia’s ethanol extract comparedto honey on changing of blood glucoselevel on diabetic model Wistar rats. Methods:This was experimental research with pre and post test controlled group design. Samples were 32 male rats, Wistar Strain, 6-8 weeks and + 200 gweight. Sampling technique used in this research was incidental sampling. Samples were divided into 4 groups: negative control group (aquadest), positive control (glibenclamide dose 0.09 mg/200 g body weight), treatment group 1 (stevia’s ethanol extract dose 20 mg/200 g body weight), treatment group 2 (honey dose 2 ml/200 g body weight). Data were analyze using Saphiro-Wilk’s test of normality and Friedmann test (α = 0.05) and Post Hoc Multiple Comparison (Wilcoxon) (α = 0.05) using SPSS 17 for Windows. Results:There was a significant difference (p = 0.002) on the Wistar’s blood glucose level which was given stevia’s ethanol extract or honey. Stevia’s ethanol extract effect more on decreasing the blood glucose than honey. But, the both of them are able to decrease the Wistar’s blood glucose on diabetic model Conclusions:Stevia’s ethanol extract has the effect of decreasing blood glucose level on diabetic model Wistar rats better than honey. Keywords:ethanol extract of stevia, honey, alloxan, changes in blood glucose levels.
Pengaruh Pemberian Ekstrak Kulit Manggis (Garcinia Mangostana L.) Dalam Mencegah Peningkatan Kadar Proteinuria Tikus Putih (Rattus Norvegicus) Yang Diinduksi Gentamisin Anggraini, Asih; Febrinasari, Ratih Puspita; Hikmayani, Nur Hafidha
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background    : The increasing use of nephrotoxic drugs has increased the incidence of renal diseases. Research on herbs with potential nephroprotective effects is needed to offer adjuvant therapy for renal diseases. Mangosteen peel contains gamma-mangostin and antioxidant which can prevent renal damages. Methods          : This research was a laboratory experiment with pre and post-test control group design. Subjects were 30 white, male, Wistar-strain rats (Rattus norvegicus), aged 2-3 months and weighed 150-230 grams. Subjects were randomly divided into 5 groups. Negative control group was treated nothing, while normal control group was treated 0.5% NaCMC. Treatment group 1, 2 and 3 were treated with mangosteen peel extract at dose 62.5 mg/kg-BW, 125 mg/kg-BW and 250 mg/kg-BW, respectively. The treatment was done for 14 days. In the last 7 days, the negative control group and the treatment groups 1, 2, and 3 were induced by gentamicin 100 mg/kg-BW. Urine samples of the subjects were collected before and after treatment to measure proteinuria levels. Data were analyzed by Kruskal-Wallis test and Mann-Whitney tests (α = 0.05) using SPSS 16.0 for Windows. Results            : Result of Kruskal-Wallis test showed that proteinuria levels differed significantly across five groups (p = 0.008). The Mann-Whitney tests showed statistically significant differences between the negative control group and the other 4 groups (p < 0.05). No significant difference was found between the normal control group and the treatment groups 1, 2, and 3 (p > 0.05). Conclusion     : Mangosteen (Garcinia mangostana L.) peel extract could prevent the increase of proteinuria levels in white rats (Rattus norvegicus) induced gentamicin. Keywords        : Mangosteen Peel Extract, Proteinuria Level, Gentamicin  
Pengaruh Pemberian Ekstrak Kulit Manggis (Garcinia mangostana L.) dalam Mencegah Kerusakan Histopatologi Ginjal Tikus Putih (Rattus norvegicus) yang Diinduksi Gentamisin Pratama, Rio Nanda; Febrinasari, Ratih Puspita; Hikmayani, Nur Hafidha
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: The use of medicinal plants has been increasing in recent years following development of herbal industry and evidence on adverse effects of several chemical drugs, including nephrotoxic effect. Research on herbs with nephroprotective effect are needed to offer alternative therapy for drug-induced renal diseases. Mangosteen pericarp extract contains xanthone which has antiinflammation effect to prevent renal damages. This research aimed to examine the nephroprotective effect of mangosteen pericarp extract on renal structure. Methods: This research was a laboratory experiment with posttest only controlled group design. Subjects were white male Wistar-strain rats (Rattus norvegicus), aged 2-3 months and weighed 150-230 grams. Subjects were randomly divided into five treatment groups. Normal control group was treated 0.5% NaCMC, while negative control group was treated nothing. The treatment groups 1, 2 and 3 were treated with extract of mangosteen pericarp at doses of 62.5 mg/kg-BW, 125 mg/kg-BW, and 250 mg/kg-BW, respectively. The treatments were maintained for 2 weeks. In the second week, the negative control group and the treatment groups 1, 2, and 3 were induced by gentamicin 100 mg/kg-BW. The subjects were later terminated and their kidneys were stained with hematoxicylin eosin. Histopathological renal damages were semi-quantitatively assessed by identification of pyknotic, karyorrhexic and karyolytic areas in proximal tubulus cells and were compared using one-way ANOVA test and followed by LSD test. Result: Result of one-way ANOVA test showed that proximal tubulus cellular damage differed significantly across five groups (p < 0.001). LSD tests further showed a significant difference between negative control group and other groups (p < 0.001). Renal damage in normal control group also differed significantly from those of treatment group 1 and 3 (p < 0.001). No significant difference was found between normal control group and treatment group 2 (p = 0.972). Conclusion: Mangosteen (Garcinia mangostana L.) pericarp extract could prevent histopathological renal damage in white rats (Rattus norvegicus) induced gentamicin. Keywords: Mangosteen pericarp extract, Renal histopathology, Gentamicin.  
Efek Nefroprotektif Ekstrak Daun Sirsak (Annona muricata L.) pada Mencit Model yang Diinduksi Parasetamol Hidayah, Nur; Suyatmi, .; Febrinasari, Ratih Puspita
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Soursop Leaf (Annona muricata L.) contains active compounds that have antioxidant effects consisting of flavonoid, alkaloid, tannin, triterpenoid and steroid. The aim of this research is to know the protective effect of Soursop Leaf Extract on the renal histological damage of  mice induced by paracetamol Methods: This study was experimental research with the post test only controlled group design. Samples were 30 male Swiss webster mice, 2-3 months old and ± 20 g of body weight. Samples were divided randomly into 5 groups, each group consisted of 6 mice. The normal group (KN) and negative control group (KKn) were given distilled water for 14 days. KP1, KP2, and KP3 were given soursop leaf extract in dose 5.6 mg, 11.2 mg, and 22.4 mg per g of body weight of mice, respectively, for 14 days. Paracetamol in dose 5.07 g per 20 g body weight of mice were given to KKn, KP1, KP2, and KP3 on the 12th ,13th ,14th days. On the 15th day mice were sacrificed and then renal organ were taken for histological preparation and stained with HE. Renal histological features were assessed based on quantifying of necrotic cells (pyknosis, karyorrhexis, karyolysis) of 50 epithelial cells from proximal tubule of right and left renal organ. Data were analyzed using One-Way ANOVA test and continued with Post Hoc (LSD) test. Results: The means of necrotic cells for KN, KKn, KP1, KP2, and KP3 were 8.17, 38.58, 26.92, 21.08 and 11.67, respectively. The One-Way ANOVA test showed a significant difference of renal damage score in five groups with p = 0.000. The Post Hoc LSD test also showed significant difference among five groups, p = 0.000. Conclusion: Soursop leaf extract can prevent the histological damage of renal cells on mice induced by paracetamol. Keywords: Soursop leaf extract, paracetamol, renal histological damage
Efek Hepatoprotektif Ekstrak Daun Sirsak (Annona muricata L.) Terhadap Kerusakan Histologis Sel Hepar Mencit (Mus musculus) yang Diinduksi Parasetamol Kumala, Riris Arizka Wahyu; Suyatmi, .; Febrinasari, Ratih Puspita
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: Soursop leaves (Annona muricata L.) contains alkaloid, flavonoid, saponin, triterpenoid, and steroid that are potent antioxidant to protect the liver from free radicals. The aim of this research was to know the hepatoprotective effect of soursop leaves extract toward liver histological damage of mice (Mus musculus) induced by paracetamol. Methods: This was an experimental laboratory research with the post test only controlled group design. Samples were 30 male Swiss Webster mice with 2-3 months old and ± 20g of weight taken by incidental sampling. They were divided randomly into 5 groups, namely kelompok kontrol negatif (KKn), kelompok kontrol (KK), kelompok perlakuan 1 (KP1), kelompok perlakuan 2 (KP2), and kelompok perlakuan 3 (KP3). KKn and KK were given aquadest for 14 days. Soursop leaves extract were given to KP1, KP2, KP3 in dose 5.6 mg, 11.2 mg, and 22.4 mg/20gbw of mice respectively for 14 days. Paracetamol in dose 5.07 g/20gbw were given to KK, KP1, KP2, and KP3 on the 12th, 13th, 14th day. On the 15th day, mice were sacrificed and their livers were taken for histological preparation with paraffin block method and HE staining. Liver histological features were assessed based on quantifying of pyknosis, karhyorhexis, and karyolysis of each 100 cells on the 3rd zone. Data were analyzed using the One-Way ANOVA test (α = 0,05) and continued with Post Hoc (LSD) test (α = 0,05). Results: The results of One-Way ANOVA test showed a significant difference of liver damage score among groups, *p = 0.000. Results of LSD test showed significant difference between KKn-KK, KKn-KP1, KKn-KP2, KKn-KP3, KK-KP1, KK-KP2, KK-KP3, KP1-KP2, KP1-KP3, KP2-KP3 with *p = 0.000 (p < 0.05). Conclusion: Soursop leaves extract (Annona muricata L.) can prevent liver histological damage of mice (Mus musculus) induced by paracetamol.Keywords: Soursop leaves extract, paracetamol, histological damage of liver cell
The Relationship between Knowledge about Antibiotics with Usage Frequency of Non-prescription Antibiotics on Non-Medical Student of Sebelas Maret University Amira, Nadya Kemala; Prasetyawati, Arsita Eka; Febrinasari, Ratih Puspita
Nexus Kedokteran Komunitas Vol 5, No 1 (2016): Nexus Kedokteran Komunitas
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

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Background: Antibiotics are the drugs most widely used and abused in the world. The most frequent form of antibiotic misuse is self-medication by various groups of society including students. Lacking of knowledge of the students, especially non-medical students, about antibiotics plays an important role in the discovery of many cases of antibiotics misuse. This study aimed to investigate the relationship between knowledge about antibiotics with usage frequency of non-prescription antibiotics on non-medical student of Sebelas Maret University. Method: This study was an observational analytic study with cross sectional approach. Sample collected from 186 non-medical students from 5 faculties in Sebelas Maret University which were randomly drawn by cluster random sampling technique. Each respondent was given a questionnaire regarding knowledge about antibiotics and the usage frequency of non-prescription antibiotics that has been tested for validity and reliability by researcher. The research data were tested with the nonparametric Spearman correlation test (α = 0.05). Result: Spearman correlation test results a significant correlation between knowledge about antibiotics with usage frequency of non-prescription antibiotics on non-medical students of Sebelas Maret University with p < 0.05 (p = 0.000). The test results also showed a strong correlation between the two variables with a correlation coefficient (r) -0.681, with increased knowledge about antibiotics will decrease the usage frequency of non-prescription antibiotics on non-medical students of Sebelas Maret University. Conclusion: There was a strong relationship between knowledge about antibiotics with the usage frequency of non-prescription antibiotics on non-medical students of Sebelas Maret University. Better knowledge of antibiotics, would reduce the usage frequency of non-prescription antibiotics. Keywords: antibiotics, knowledge about antibiotics, the usage frequency of non-prescription antibiotics
Co-Authors . Suyatmi . Suyatmi . Widardo ., Hudiyono ., Muthmainah ., Suyatmi A.A. Ketut Agung Cahyawan W Adi Magna Patriadi Nuhriawangsa AGYA GHILMAN FAZA Ahmad Ahid Mudayana Ahmad Ahid Mudayana Akhmad Azmiardi Alfi Yasmina Ali, Agni Shalha Amer Abdulwahab Al-aghbari, Nuha Amira, Nadya Kemala Anggraeni, Selvia Anis Nurhidayati Anshari, Shafira Yasmine Argyo Demartoto, Argyo Ari Natalia Probandari Ari Probandari Arsita Eka Prasetyawati Arsita Eka Prasetyawati Asaduddin, Aiman Hilmi Asih Anggraini Asih Anggraini Astrida Fesky Febrianty Astrika Yunita, Fresthy Bachnas, Muhammad Adrianes Berlian Permata Sakti Bhisma Murti Binti Maulina Binti Maulina Putri Brian Wasita Brian Wasita Brian Wasita Bustami, Mursyid Chintya Wulandarie Colti Sistiarani Colti Sistiarani Dara, Rizki Wulan Dedi Febriandaru Desy Puspa Putri Devi Ratna Mayasari DIAN LESTARI Didik Gunawan Tamtomo Dilma’aarij, Dilma’aarij Dyonisa Nasirochmi Pakha Endang Listyaningsih Suparyanti Erna Kristin Ernawati, Dwi Arini Eti Poncorini Pamungkasari FAZA, AGYA GHILMAN Hamka, Dwi Wulandari Aaas Hanif Mustikasari Harioputro, Dhani Redhono Hariyanti, Lusiana Pradana Hartono Hartono Haruni, Diah Sekar Heni Hastuti Hudiyono . Hutami Sri Ummiyati Ida Nurwati Ignatius Gonggo Prihatmono Imanisa, Octavia Intan Indarta, Ardhia Fefrine Irma Prasetyowati iwan Dwiprahasto Kenneth Tan Khoiriyah, A'maliatul Kisrini . Komang Windayani Kristiani, Shinta Laksmi Wardhani, Tri Martya Ningrum Leli Saptawati Lilik Wijayanti Lilik Wijayanti Lilik Wijayanti Wijayanti Lisyawati, Shanti Lukman Aryoseto Ma'rifatul Khoiriyah Ayda Maryati, Warsi Mashuri, Yusuf Ari Meintansari Manik MIFTAKHUL JANNAH Mochammad Fairuz Zahran Muchsin Doewes Mustara, Mustara Mustiqa Febriniata MUTHMAINAH . Muthmainah Muthmainah Nadya Kemala Amira Nafi'ah, Radifatun Naila Shofwati Putri Nanang Wiyono Nila Authoria Authoria Nita Dwi Astikasari Novelia Qothrunnada Novika, Revi Gama Hatta Nur Hafidha Hikmayani Nur Hafidha Hikmayani Nur Hasanah, Laras Eka Nur Hidayah Nurhasan Agung Prabowo Nurholis Majid Oktaviani oktaviani Paramasari Dirgahayu Paramasari Dirgahayu Poncorini Pamungkasari, Eti Primaningtyas, Widana Putri, Naila Shofwati Rahardjo, Setyo Sri Rio Nanda Pratama Rio Nanda Pratama Riris Arizka Wahyu Kumala Riris Arizka Wahyu Kumala, Riris Arizka Wahyu Rizaldy Pinzon RR. Ella Evrita Hestiandari Sakti, Berlian Permata Saptawati, Leli Satwika, Ariq Ratya Selvia Anggraeni Setyani, Rizka Ayu Setyani, Rizka Ayu Setyo Sri Rahardjo Setyo Sri Rahardjo Shinta Kristianti Shinta Retno Wulandari Shinta Shinta Sigit Setyawan Siswahyudianto Siti Fatimah R Solikah, Tri Agusti Sopianti, Densi Selpia Sri Darnoto Sri Mulyani Sri Mulyani Sugiarto Sumandjar, Tatar Sumardiyono Sumardiyono Sumardiyono Sumardiyono Suparyanti, Endang Listyaningsih Suparyanti, Endang Listyaningsih Susanto, Agnes Suyatmi . Syamsiar, Syamsiar Tri Agusti Sholikah Uki Retno Budihastuti Ummiyati, Hutami Sri Veronika Ika Budiastuti Vitri Widyaningsih Vitri Widyaningsih Wahyu Anjas Sari Wasita, Brian Widardo . Windayani, Komang Wiyono, Nanang Wulandari, Shinta Retno Yanuarini, Triatmi Andri Yulia Lanti Retno Dewi Yulia Sari Zahran, Mochammad Fairuz