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All Journal Stannum : Jurnal Sains dan Terapan Kimia Asian Journal of Health and Applied Sciences Riset Informasi Kesehatan
Pakpahan, Suyarta Efrida
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Phylogenetic analysis of the SARS-CoV-2 virus gene-based on the chain A glycoprotein spike in Wuhan Pakpahan, Suyarta Efrida; Sari, Ira Prima; Koesmawati, Dea
Asian Journal of Health and Applied Sciences Vol. 1 No. 2 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.266 KB) | DOI: 10.53402/ajhas.v1i2.6

Abstract

The spread of COVID-19 in various countries has increased the death toll due to COVID-19. Spike glycoproteins of the SARS-CoV-2 virus have an important role in binding to host cell receptors. This spike can identify the SARS-CoV-2 kinship in Wuhan and other countries by phylogenetic analysis. This study aims to determine the phylogenetic relationship of COVID-19 from Wuhan with the other countries obtained from the NCBI Gene bank based on spike chain A glycoproteins. The method uses NCBI's BLAST program to search for similar sequences, ClustalW's program to perform multiple alignment sequencing, and MegaX to create a phylogenetic tree. The research results Spike glycoprotein chain A in Wuhan has the closest kinship with the United States. This is indicated by the formation of tree branches close to each other but still in the same group as spike glycoproteins in other countries. Phylogenetic tree validated by the bootstrap test that value of 100%, which means it shows the sturdiness of the tree can be trusted. The conclusion is there is no significant difference in the characteristics of spike glycoprotein chain A, as for some countries that have spike amino acid differences in glycoprotein chain A, such as Pakistan, Poland, and Wuhan. The amino acid difference is considered normal because the virus will continue to evolve in order to adapt to the environment
Topical hydrogel human amnion membrane for wounds healing in mice (mus musculus) induced by diabetes Pakpahan, Suyarta Efrida; Fadhilah, Fitri Rahmi; Amalia, Mia; Putri, Ericha Nurlina
Riset Informasi Kesehatan Vol 14 No 2 (2025): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30644/rik.v14i2.1013

Abstract

Background: One of the complications that occurs in DM patients is the occurrence of chronic wounds. The use of Human Amnion Membrane (hAM) has been identified as a promising natural option for the treatment of skin lesions, including burns and diabetes mellitus. This hAM contains an extracellular matrix (ECM) with components such as growth factors, collagen, and proteoglycans as anti-inflammatory and anti-microbial in wounds. The purpose of this study was to analyze the effect of hAMD hydrogel treatment on wound closure in mice induced by alloxan macroscopically and microscopically on days 3, 7, 14, and 21. Method: This research method, experimental using mice divided into 4 treatment groups, namely negative control (healthy group without treatment, DM without treatment), internal control (DM treatment carbopol) and DM treatment hAMD. Measurement of wound area (macroscopic), histology processing (Hematoxylin Eosin), reading of histology preparations (microscopic) and Two-way ANOVA test analysis using Graphpad Prism 10 was carried out. Results: The results of the study from 4 treatment groups where in the healthy group without treatment on the 21st day the wound closure was complete, the DM group without treatment on the 21st day the wound closure was not yet completely closed, in the DM carbopol treatment group on the 14th day the wound closure was not yet completely closed, and in the DM hAMD treatment group 15% on the 14th day the wound closure had closed completely. This is in line with the results of statistical and histological analysis. Conclusion: The conclusion of this study is that the effect of human amnion membrane hydrogel can heal diabetes mellitus skin wounds faster compared to the control group. Keywords: Diabetes Mellitus, Hydrogel, Extracellular Matrix, Amniotic Membrane.
Ekstrak Daun Meniran (Phyllanthus niruri L.) : Bagaimana Pengaruhnya Terhadap Jumlah Trombosit di Dalam Darah? Sumiati, Risma Laila; Baehaki, Farhan; Nurdin, Nurdin; Pakpahan, Suyarta Efrida
Stannum : Jurnal Sains dan Terapan Kimia Vol 6 No 1 (2024): April 2024
Publisher : Department of Chemistry - Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jstk.v6i1.4401

Abstract

Thrombocytopenia is a condition in which the platelet count is below normal (<150,000-450,000 cells/mm3). Until now, the treatment of thrombocytopenia has not yet found a specific drug and there are side effects from chemical treatment, so alternative solutions are needed, one of which is the use of meniran leaves (Phyllanthus niruri L.). The meniran plant is a wild plant that is often found in the wild, on the streets, as well as vacant land so that people can easily get these plants. The aim of this research was to determine the effect of meniran leaf extract (Phyllanthus niruri L.) on the number of platelets in mice induced by sodium phenytoin. The method used in this research was True Experimental with Pretest and Posttest with Control Group Design consisting of 5 groups which aimed to determine the effect of meniran leaf extract on platelet counts. The data collection technique used in this study used primary data, which was obtained from the results of examining platelet counts on day 0, day 3 after induction and day 7 after treatment with meniran leaf extract (Phyllanthus niruri L.). Then the data obtained was tested using SPSS with the non-parametric Kruskal-Wallis and Mann-Whitney tests. From 25 experimental animals, the results showed that there was a relatively significant increase in the number of platelets after being given therapy with meniran leaf extract (Phyllanthus niruri L.) but there was no difference between each dose. Based on the research results, it can be concluded that meniran leaf extract (Phyllanthus niruri L.) has an effect on increasing the number of platelets.
Co-Authors Farhan Baehaki Fitri Rahmi Fadhilah, Fitri Rahmi Koesmawati, Dea Mia Amalia Nurdin Nurdin Putri, Ericha Nurlina Sari, Ira Prima Sumiati, Risma Laila