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All Journal The Indonesian Journal of Infectious Diseases Asian Journal of Health and Applied Sciences Riset Informasi Kesehatan
Fitri Rahmi Fadhilah, Fitri Rahmi
Departemen Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Padjadjaran
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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The Effect of Different Extraction Temperatures of SARS-COV2 RT-PCR Examination Process On CT Value In Patient Specimens In Labkesda Kabupaten Sukabumi, Indonesia Fadhilah, Fitri Rahmi; Wahid, Aziz Ansori; Rachman, Mochamad Irwan Fitriana
Asian Journal of Health and Applied Sciences Vol. 1 No. 3 (2022): Asian Journal of Health and Applied Sciences (AJHAS)
Publisher : Lighthouse Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53402/ajhas.v1i3.39

Abstract

The Severe Acute Respiratory Syndrome Coronavirus-2, or COVID, was announced by WHO on February 11, 2020.  Examination of SARS-CoV2 by RTq-PCR method in Labkesda Kab. Sukabumi is one of the health service support units in the Sukabumi area. The number of PCR examination requests is increasing every day, whereas, at this stage, the temperature increase process should reach the recommended temperature of 90°C, which can take 20-25 minutes. Sometimes ATLM has processed specimens at 60°C - 90°C. Given the required temperature at the time of extraction, which is at a temperature of 90 degrees, here the researchers wanted to prove whether below 90 degrees had an effect on the virus extraction process. This study aims to determine the effect of differences in extraction temperature on the SARS-CoV2 RTq-PCR examination process on CT VALUE on patient specimens. The variable in this study was the difference in temperature consisting of 60°C, 80°C, and 90°C. This research used quantitative research methods with case studies. This study is an experimental study to determine the effect of temperature differences on the CT value of the RTq - PCR examination. The population of this study were 30 patients at LABKESDA Kabupaten Sukabumi. This analysis used a one-way ANOVA test analysis with software. IBM SPSS 23.0. It may be concluded that there is no significant difference in the CT Value findings on the SARS-CoV2 RTq-PCR Examination since the study's p-value was 0.758, which is more than 0.05.
Potential of the vitexin compound in Kecombrang Kombucha Flowers (Etlingera elatior) as a candidate for antidiabetic, colon cancer and anti-inflammatory drugs based on bioinformatics analysis: Potensi Senyawa Viteksin pada Bunga Kombucha Kecombrang (Etlingera elatior) Sebagai Kandidat Obat Antidiabetes, Antikanker Usus Besar dan Antiinflamasi Berdasarkan Analisis Bioinformatika Fadhilah, Fitri Rahmi; Rezaldi, Firman; Masruroh, Nazilatul; Khaerunnisa Putri, Salsabila; Nur Hafizah, Rahmi
Riset Informasi Kesehatan Vol 14 No 1 (2025): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30644/rik.v14i1.899

Abstract

Topical hydrogel human amnion membrane for wounds healing in mice (mus musculus) induced by diabetes Pakpahan, Suyarta Efrida; Fadhilah, Fitri Rahmi; Amalia, Mia; Putri, Ericha Nurlina
Riset Informasi Kesehatan Vol 14 No 2 (2025): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30644/rik.v14i2.1013

Abstract

Background: One of the complications that occurs in DM patients is the occurrence of chronic wounds. The use of Human Amnion Membrane (hAM) has been identified as a promising natural option for the treatment of skin lesions, including burns and diabetes mellitus. This hAM contains an extracellular matrix (ECM) with components such as growth factors, collagen, and proteoglycans as anti-inflammatory and anti-microbial in wounds. The purpose of this study was to analyze the effect of hAMD hydrogel treatment on wound closure in mice induced by alloxan macroscopically and microscopically on days 3, 7, 14, and 21. Method: This research method, experimental using mice divided into 4 treatment groups, namely negative control (healthy group without treatment, DM without treatment), internal control (DM treatment carbopol) and DM treatment hAMD. Measurement of wound area (macroscopic), histology processing (Hematoxylin Eosin), reading of histology preparations (microscopic) and Two-way ANOVA test analysis using Graphpad Prism 10 was carried out. Results: The results of the study from 4 treatment groups where in the healthy group without treatment on the 21st day the wound closure was complete, the DM group without treatment on the 21st day the wound closure was not yet completely closed, in the DM carbopol treatment group on the 14th day the wound closure was not yet completely closed, and in the DM hAMD treatment group 15% on the 14th day the wound closure had closed completely. This is in line with the results of statistical and histological analysis. Conclusion: The conclusion of this study is that the effect of human amnion membrane hydrogel can heal diabetes mellitus skin wounds faster compared to the control group. Keywords: Diabetes Mellitus, Hydrogel, Extracellular Matrix, Amniotic Membrane.
Potensi Antimikroba Pada Teh Kombucha Bunga Kecombrang (Etlangia elatior) Fadhilah, Fitri Rahmi; Pakpahan, Suyarta; Rezaldi, Firman; Kusmiran, Eny; Cantika, Erika; Julinda, Olvia; Muhammad, Riszal
The Indonesian Journal of Infectious Diseases Vol. 10 No. 1 (2024): The Indonesian Journal of Infectious Diseases
Publisher : Rumah Sakit Penyakit Infeksi Prof Dr. Sulianti Saroso

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32667/ijid.v10i1.186

Abstract

Latar Belakang: Salah satu minuman probiotik yang terbuat dari bunga kecombrang yang difermentasi bersama scoby (biang kombucha), dan substrat gula disebut kombucha teh bunga kecombrang. Peningkatan konsentrasi gula dapat menghambat pertumbuhan bakteri dan jamur patogen. Tujuan penelitian ini adalah untuk mengetahui pengaruh kombucha bunga kecombrang terhadap aktivitas pertumbuhan bakteri patogen gram positif dan gram negatif serta jamur patogen berdasarkan variasi konsentrasi gula. Perlakuan konsentrasi gula yang digunakan adalah 20%, 30%, dan 40%. Metode: Penelitian menggunakan metode difusi cakram dengan menggunakan bakteri uji Escherichia coli dan Staphylococcus aureus serta jamur uji Candida albicans untuk mengetahui diameter zona bening yang terbentuk berdasarkan  sifat anti mikroba kombucha. Hasil: Hasil penelitian menunjukkan bahwa konsentrasi 40% merupakan konsentrasi gula pada fermentasi kombucha bunga kecombrang yang paling baik dalam menghambat pertumbuhan Staphylococcus aureus, Escherichia coli, dan Candida albicans dengan diameter masing-masing 14 mm, 10,3 mm dan 12 mm. Setelah data digabungkan dengan zona hambat terhadap C. albicans, diperoleh hasil yang berbeda nyata pada konsentrasi gula 40% dengan p-value (0,000). Kesimpulan: Fermentasi kombucha teh bunga kecombrang mempunyai spektrum yang luas dan berpotensi sebagai minuman probiotik dalam meningkatkan imunitas dengan menghambat laju pertumbuhan bakteri patogen dan ragi.
Co-Authors Cantika, Erika Fitri Rahmi Fadhilah Julinda, Olvia Khaerunnisa Putri, Salsabila Krisdaryani, Rizka Kusmiran, Eny Masruroh, Nazilatul Mia Amalia Muhammad, Riszal Nur Hafizah, Rahmi Pakpahan, Suyarta Pakpahan, Suyarta Efrida Putri, Ericha Nurlina Rachman, Mochamad Irwan Fitriana Rezaldi, Firman Wahid, Aziz Ansori