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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Administrasi Pemerintahan Desa
Rustini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Materials Science & Nanotechnology Physics Social Sciences

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Kualitas Pelayanan Publik di Kantor Kalurahan Srihardono Kapanewon Pundong Kabupaten Bantul Rustini; Khozin, Muhammad
Jurnal Administrasi Pemerintahan Desa Vol. 6 No. 2 (2025): Agustus
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/villages.v6i2.289

Abstract

Penelitian ini bertujuan untuk mengetahui kualitas pelayanan publik yang diselenggarakan oleh kantor Kalurahan Srihardono Kapanewon Pundong Kabupaten Bantul dan untuk memberikan rekomendasi agar Kalurahan Srihardono dapat melakukan perbaikan terhadap pelayanan publik yang diselenggarakan. Sehingga dapat mewujudkan pelayanan yang berkualitas sebagai ciri pemerintahan yang baik. Metode yang digunakan dalam penelitian adalah metode pendekatan kuantitatif-deskriptif. Penelitian ini menggunakan instrumen berupa kuesioner yang diberikan kepada masyarakat yang menerima layanan di Kalurahan Srihardono. Sampel penelitian ini berjumlah 91 responden yang ditentukan dengan teknik accidental sampling. Kualitas pelayanan dianalisis menggunakan 5 (lima) dimensi model SERVQUAL yaitu bukti fisik (tangibles), kehandalan (reliability), ketanggapan (responsiveness), jaminan (assurance) dan empati (empathy). Hasil penelitian menunjukkan bahwa kualitas pelayanan publik di kantor Kalurahan Srihardono secara umum berada pada kategori sangat baik dengan nilai rata-rata 81,59%. Dimensi empati memperoleh nilai tertinggi yaitu 84.07%, sedangkan dimensi ketanggapan dan jaminan memiliki nilai paling rendah. Temuan penelitian ini menunjukkan pentingnya penyusunan SOP layanan, peningkatan kompetensi, kredibilitas, dan integritas pegawai guna mendukung pelayanan publik yang transparan, efektif, efisien dan profesional.
Aktivitas Penghambatan Enzim α-Glukosidase dari Ekstrak Hasil Fermentasi Bakteri Endofit dari Tapak Dara (Catharanthus roseus (L.) G. Don) Daun Huda, Ulfa Rosiatul; Ismed, Friardi; Arifah, Nurwahidatul; Sardy, Valdy Filando; Rustini
Jurnal Penelitian Pendidikan IPA Vol 10 No 10 (2024): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i10.9178

Abstract

Catharanthus roseus (L.) G. Don contain bioactive compounds that act as inhibitors against the α-glucosidase enzyme. This study aims to test the α-glucosidase enzyme inhibitor activity of fermented extract of endophytic bacteria from tapak dara leaves and identify the bacterial species. Endophytic bacteria were isolated using a direct planting method, resulting in eight isolates. The bacteria were fermented for 24-72 hours in nutrient broth media. Ethyl acetate was used to extract the fermented bacteria. Screening for α-glucosidase enzyme inhibitory activity was conducted using the TLC bioautography method. Four bacterial isolates (TD 1, TD 3, TD 4, TD 7) produced clear zones, qualitatively indicating that the isolates have inhibitory activity against the α-glucosidase enzyme. The IC50 results from the eight isolates showed that three isolates provided strong IC50 values: isolate TD 1 at 19.93 µg/ml, isolate TD 2 at 92.34 µg/ml, and isolate TD 4 at 13.94 µg/ml, compared to acarbose at 48.85 µg/ml). Molecular identification showed that isolate TD 1 was Pseudomonas oryzihabitans and isolate TD 4 was Staphylococcus warneri. This study concluded that endophytic bacteria from tapak dara leaves have the potential as a source of α-glucosidase enzyme inhibitor compounds for the development of antidiabetic drugs.
Isolasi dan Pengujian Aktivitas Penghambat Enzim Alfa Glukosidase dari Bakteri Endofit Daun Kumis Kucing (Orthosiphon aristatus (Blume) Miq.) Putri, Indah Tamara Herman; Rustini; Ismed, Friardi; Sardi, Valdy F; Arifah, Nurwahidatul
Jurnal Penelitian Pendidikan IPA Vol 10 No 10 (2024): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i10.9264

Abstract

Orthosiphon aristatus (Blume) Miq., commonly known as kumis kucing, is frequently used in traditional Indonesian medicine to treat diabetes, hypertension, rheumatism, gout, and as a diuretic. However, large-scale production of medicinal products from natural sources requires significant raw material and land, creating a need for alternative methods. This study explores the potential of isolating endophytic bacteria from kumis kucing leaves to serve as α-glucosidase enzyme inhibitors, offering a more sustainable approach. Eight bacterial endophytes were isolated and tested using bioautography assays and IC50 measurements to assess their inhibitory activity. Four isolates—KK 1, KK 3, KK 4, and KK 5—demonstrated significant α-glucosidase inhibition, with IC50 values of 41.35 µg/mL, 57.04 µg/mL, 56.70 µg/mL, and 164.16 µg/mL, respectively. Molecular testing identified KK 1 as Priestia aryabhattai, while KK 3 and KK 5 were identified as Priestia megaterium. These findings suggest that endophytic bacteria isolated from Orthosiphon aristatus leaves have the potential to be developed as natural sources of α-glucosidase inhibitors, which are beneficial for diabetes management.
Co-Authors Arifah, Nurwahidatul Friardi Ismed Huda, Ulfa Rosiatul Muhammad Khozin Putri, Indah Tamara Herman Sardi, Valdy F Sardy, Valdy Filando