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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Veteriner ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) VOGELKOP: Jurnal Biologi Jurnal Natural

Hermawaty Abubakar

Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Papua, Jl. Gunung Salju, Amban, Manokwari, Provinsi Papua Barat, Indonesia, 98134

Author-ID : 218479

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Mathematics Physics Veterinary

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Articles

Isolasi Bakteri Penghasil Indole Acetic Acid pada Tanaman Hortikultura di Perkebunan Prafi SP 1, Manokwari Rina Anita Mogea; Waode Intan Cendrasari La Halim Putri; Hermawaty Abubakar
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 1 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.1.1

Horticultural plants are branches of agriculture that are associated with crop cultivation such as vegetables, fruit, various ornamental plants, and medicinal plants that require compounds to accelerate the formation of roots which can be done by using growth regulators. One of the growth regulators needed for plants is auxin or Indole Acetic Acid (IAA). The presence of bacteria that act as producers of IAA can be used to stimulate the growth of horticultural plants. Therefore, the exploration of bacteria that have the potential to produce auxin in the future can provide an important and significant role in increasing agricultural production. This study aims to isolate, select, measure the value of IAA, and characterize IAA-producing bacteria. Isolation of auxin-producing bacteria using general media of growth bacteria, i.e., Nutrient Agar (NA) and Nutrient Broth (NB). The location for sampling of rhizosphere soil was Prafi SP 1 Plantation, Manokwari, Indonesia. The results showed that as many as 9 bacterial isolates were obtained from the rhizosphere of horticultural plants. After selection of auxin-producing bacteria, 2 bacteria isolates (IBPA 3 and IBPA 5) were obtained which were able to produce IAA. IAA concentration value in IBPA 3 isolate is 42.178 ppm and in IBPA 5 isolate is 27.65 ppm. The characteristics of IBPA 3 isolate refer to the genus Bacillus and IBPA 5 isolate refer to the genus Pseudomonas. Keywords: agriculture, bacteria rhizosphere, indole acetic acid

Uji Aktivitas Senyawa Antibakteri Katak Papua (ANTIBACTERIAL ACTIVITY TEST OF PAPUAN FROGS) Maria Massora; Elda Irma J. J. Kawulur; Hermawaty Abubakar
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Skin and submental glands of Papuan frogs have antibacterial compounds‘ which were capable to impede growth of tested bacteria based on diffusion method. These frogs were Platymantis papuensis, Litoria infrafrenata, Bufo melanostictus, Rana grisea and Rana sp., and the tested bacterial were the positive Gram bacteria (Staphylococcus aereus, and Bacillus subtilis) and the negative Gram bacteria (Escherichia coli and Pseudomonas aeroginosa). Skin frog which extracted with hot water (100oC) showed antibacterial activity which significantly impeded the growth of tested bacteria using diffusion method.

Skrining Bakteri yang Berasosiasi dengan Spons Jaspis sp. Sebagai Penghasil Senyawa Antimikroba Hermawaty Abubakar; Aris Tri Wahyudi; Munti Yuhana
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 1 (2011): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Organisme bentik laut seperti spons, seringkali hidup berasosiasi dengan bakteri yang menghasilkan senyawa antimikroba. Penelitian ini bertujuan untuk mengetahui kemapuan antagonis isolat-isolat bakteri yang berasosiasi dengan spons Jaspis sp. terhadap beberapa bakteri patogen, dengan metode skrining secara kualitatif. Sebanyak 32 (45,71%) dan 20 (29,41%) isolat yang berasal dari bagian mesohyl dan permukaan Jaspis sp. menunjukkan kemampuan antimikroba, karena mampu menghambat pertumbuhan Staphylococcus aureus, Vibrio harveyii, Escherichia coli, Pseudomonas aerogenosa, EPEC K-11, Candida albicans, and C. tropicalis. Uji fenotipik dilakukan pada beberapa isolat dengan aktivitas antimikroba terbaik, yaitu SAB E-8, SAB E-33, SAB E-35, SAB E-38, SAB E-40 dan SAB S-43. Hasil pewarnaan Gram menujukkan isolat SAB E-8, SAB E35, and SAB E-40 adalah Gram negatif, sedangkan isolat SAB E-33, SAB E-38, and SAB S-43 adalah gram positif yang dilanjutkan dengan identifikasi parsial (pengecatan gram dan uji katalase) untuk kelompok Bacillus.Kata kunci: Bakteri, Asosiasi, Jaspis sp., antimikroba Living benthic marine organisms such as sponges are frequently assosiated with as bacteria that may be produce antimicrobial compounds. This study aims to determine antagonistic of bacterial isolates that associated sponge Jaspis sp., with a qualitative screening method. Screening of bacteria from marine sponge Jaspis sp. which have bility to produce antibacterial subtances was investigated. There are 32 (45,71%) and 20 (29,41%) isolates from mesohyl and surface sponge respectively. Those isolated bacterial showed the antibacterial activity against Staphylococcus aureus, Vibrio harveyii, Escherichia coli, Pseudomonas aerogenosa, EPEC K-11, Candida albicans, and C. tropicalis. However, use of a few additional simple phenotypic tests for those isolate can be used to differentiate among isolates. The simple phenotypic test divided two ways based on staining gram. Gram negative bacteria were desingned SAB E-8, SAB E-35, and SAB E-40 and gram positive bacteria were desingned SAB E-33, SAB E-38, and SAB S-43. Parsial identification that directed to Bacillus was used for positive gram bacteria, involve gram staining, endospora staining and katalase test. Key words: Bacteria, Assosiation, Jaspis sp, antimicrobe

Genetic analisys of cytochrome oxidase sub unit 1 gene fragment from Cirrhilabrus cf. ryukyuensis (Labridae) from Cenderawasih Bay and Raja Ampat Muhammad Dailami; Darma Santi; . Murtihapsari; Hermawaty Abubakar; Abdul Hamid A. Toha
Jurnal Iktiologi Indonesia Vol 18 No 3 (2018): October 2018
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v18i3.347

Cirrhilabrus cf. ryukyuensis from Cenderawasih Bay and Raja Ampat have different colormorphs with Cirrhilabrus ryukyuensis. This study was conducted to determine the phylogenetic relationship, evolution and genetic diversity of Cirrhilabrus cf. ryukyuensis based on Cytochrome oxidase sub unit 1 (COI) gene. Mitochondrial DNA extracted by using chelex 10%. FISH-BCH and BCL primers were used to amplify COI gene fragment and sequencing. Data analysis conducted by using software Sequencher 4.1 and MEGA 5. COI genes from six samples of C. cf rykyuensis from Cenderawasih Bay and Raja Ampat have total 613 base pairs. The average compositions of nucleotides are T 32.8%, C 26.3%, A 23.2% and G 17.7%. There is six positions of polymorphism that caused by transition. Conversion of nucleotides to amino acid sequence resulted in 204 amino acid with the same sequence. The phylogenetic tree from six sample showed that all in one clade, but different clade from Cirrhilabrus cyanopleura from GenBank Abstrak Cirrhilabrus cf. ryukyuensis asal Teluk Cenderawasih dan Raja Ampat secara morfologi memiliki perbedaan warna dengan Cirrhilabrus ryukyuensis. Penelitian ini bertujuan menentukan hubungan filogenetik, evolusi, dan keragaman genetik Cirrhilabrus cf. ryukyuensis berdasarkan gen Sitokrom Oksidase sub Unit I (COI). Genom DNA mitokondria diekstrak menggunakan larutan chelex 10%. Primer FISH-BCH dan BCL digunakan untuk mengamplifikasi fragmen Gen COI dan sekuensing. Analisis data dilakukan dengan menggunakan perangkat lunak Sequencher 4.1 dan MEGA 5. Gen COI dari enam sampel C. cf. rykyuensis asal Teluk Cenderawasih dan Raja Ampat, memiliki panjang basa men-capai 613 bp. Komposisi rata-rata nukleotida T 32,8%, C 26,3%, A 23,2% dan G 17,7%. Terdapat enam posisi poli-morfisme yang kesemuanya disebabkan oleh mutasi transition. Hasil penerjemahan nukleotida, diperoleh asam amino dengan panjang 204 asam amino dengan urutan yang sama. Pohon filogenetik menunjukkan keenam sampel berada dalam satu clade yang sama dan berbeda dari clade Cirrhilabrus cyanopleura data Gene Bank.

AMPLIFIED RIBOSOMAL DNA RESTRICTION ANALYSIS (ARDRA) BAKTERI DENGAN POTENSI ANTIMIKROB YANG BERASOSIASI DENGAN SPONS Jaspis sp. Hermawaty Abubakar
Jurnal Natural Vol. 9 No. 1 (2010)
Publisher : FMIPA Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/jn.v9i1.32

Sponges are one of the components that compose coral reef which have a potential bioactive substance that has not been utilized. Sponges are generally able to survive in marine waters were nutrients are poor because of associations with other organisms, especially bacteria. This study aimed to isolate and characterize bacteria (endosymbiont and ectosimbion) that produce antimicrobial compounds, and analyze genetic diversity based on Amplified Ribosomal DNA Restriction Analysis (ARDRA). The results of isolation obtained 138 bacterial isolates, which are 70 endofit isolates and 68 surfaces isolates respectively. The results obtained, based on antimicrobial test, was 32 bacterial isolates (45.71%) of the total bacterial isolates that have endofit antimicrobial activity, whereas on the surface bacteria, 20 bacterial isolates (29.41%) of the total surface of the bacterial isolates also have antimicrobial activity. Genetic diversity was carried out on 30 isolates that has the best antimicrobial activity. Amplyfication of 16S rRNA gene is done using specific primers, 63f and 1387r. The profile of 16S rRNA gene band shows a high diversity, which is generated after cutting with three restriction enzymes i.e. RsaI, HaeIII and HinfI. The three restriction enzymes have different cuts and properties. Construction of phylogenetic trees based on analysis of Amplified Ribosomal DNA restriction, grouped 30 isolates from the sponge Jaspis sp. which have a microbial activity on seven filotipe. This grouping is based on the similarities cuts of sites of each isolate after restriction by three different restriction enzymes.

UJI AKTIVITAS ENZIM SELULASE ISOLAT BAKTERI DARI SEDIMEN LAMUN PERAIRAN RENDANI MANOKWARI Tirza Bandi; Hermawaty Abubakar; Rina A Mogea
VOGELKOP: Jurnal Biologi Vol 1, No 1 (2018)
Publisher : Jurusan Biologi, FMIPA, Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/vogelkopjbio.v1i1.34

ABSTRACTIndonesia has the potential of renewable natural resource which are abundant both in number and types of plants containing cellulose fiber. In natures cellulose cannot be completely degraded because it needs of microorganisms such as fungi and bacteria which will produce cellulase enzymes so that it can degrade cellulose in nature. The purpose of this study was to analysed the activity of cellulase enzymes produced by bacterial isolates obtained from seagrass sediments. Cellulase activity of 11 bacterial isolates was determined by the cellulotic index value passing through the Congo Red staining method on 1% CMC solid media. A total of seven isolates indicated a positive result and the highest index value was produced by SI-E isolates, that is 4.7 mm. After that the value of cellulase enzyme activity from the seven positive isolates was determined by counting substrate reducing sugars through the 3.5-dinitrosalicylic (DNS) method. The results showed that SI-H isolates had the highest activity of 0.071 U/mL. ABSTRAKIndonesia memiliki potensi berupa sumber daya alam terbaharukan yang melimpah baik dalam jumlah maupun jenis tumbuhan yang mengandung serat selulosa. Di alam selulosa tidak dapat terdegradasi secara sempurna oleh karena itu dibutuhkan bantuan mikroorganime seperti jamur dan bakteri yang akan menghasilkan enzim selulase sehingga dapat mendegradasi selulosa yang berada di alam. Tujuan dari penelitian ini adalah untuk menganalisis aktivitas enzim selulase yang dihasilkan oleh isolat bakteri yang digunakan. Aktivitas selulase dari 11 isolat bakteri ditentukan dengan nilai indeks selulotik melaluli metode pewarnaan merah kongo pada media padat CMC 1%. Sebanyak tujuh isolat yang menandakan hasil positif dan nilai indeks tertinggi dihasilkan oleh isolat SI-E yaitu 4.7 mm. Setelah itu nilai aktivitas enzim selulase dari ketujuh isolat positif ditentukan dengan menghitung gula pereduksi substrat melalui metode 3.5-dinitrosalisilat (DNS). Hasil menunjukkan isolat SI-H memiliki aktivitas tertinggi yaitu 0.071 U/mL.

ACTINOMYCETES ASAL RHIZOSFER TUMBUHAN MANGROVE DI KAMPUNG WAMESA DISTRIK MANOKWARI SELATAN KABUPATEN MANOKWARI SEBAGAI PENGHASIL ANTIMALARIA Tati Tati; Hermawaty Abubakar; Yenni Y Salosa
VOGELKOP: Jurnal Biologi Vol 3, No 1 (2023)
Publisher : Jurusan Biologi, FMIPA, Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/vogelkopjbio.v3i1.68

Malaria is a public health problem in Indonesia and several other countries. The rhizosphere is a natural habitat for various soil microorganisms. The mangrove rhizosphere area is very potential for microbial growth, especially Actinomycetes. This study aims to identify and test the In Vitro antimalarial ability of the best isolates isolated from the mangrove rhizosphere of Wamesa Village based on the results of tests on bioactive compounds. In this study, 3 pure Actinomycetes isolates were obtained, namely T1, T2 and T3 which were grouped into 3 three groups (colour grouping) based on the color of the aerial mycelium, the substrate mycelium, and the color that diffuses in the media. The test results for the content of the Actinomycetes bioactive compounds showed that isolates T1 and T2 contained alkaloids, saponins, flavonoids and tannins, while isolate T3 contained alkaloids, saponins and tannins. The results of the study of the antimalarial activity of Actinomycetes isolates against P. falciparum in vitro showed that isolate T1 had the highest inhibition percentage at a concentration of 10-2 μg/mL, namely 83.33%, and the lowest inhibition at a concentration of 10-12 μg/mL, namely 58.33%. Isolate T2 had the highest inhibition percentage at a concentration of 10-3 μg/mL, namely 95.38% and the lowest inhibition, namely 10-12 with an inhibition value of 64.62%. The IC50 values of isolates T1 and T2 were 1.95x10-15 µg/mL and 1.45 x 10-15 µg/mL, respectively.

ANALISIS GEN 16S rRNA ISOLAT SMSS-VII DAN SMSSe-VII YANG BERPOTENSI SEBAGAI BAKTERI PENDEGRADASI SOLAR Huliselan, Rendy Leonard; Salosa, Yenni Yendri; Abubakar, Hermawaty
VOGELKOP: Jurnal Biologi Vol 1, No 2 (2018)
Publisher : Jurusan Biologi, FMIPA, Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/vogelkopjbio.v1i2.7

Indonesia memiliki potensi berupa sumber daya alam terbaharukan yang melimpah baik dalam jumlah maupun jenis tumbuhan yang mengandung serat selulosa. Di alam selulosa tidak dapat terdegradasi secara sempurna oleh karena itu dibutuhkan bantuan mikroorganime seperti jamur dan bakteri yang akan menghasilkan enzim selulase sehingga dapat mendegradasi selulosa yang berada di alam. Tujuan dari penelitian ini adalah untuk menganalisis aktivitas enzim selulase yang dihasilkan oleh isolat bakteri yang digunakan. Aktivitas selulase dari 11 isolat bakteri ditentukan dengan nilai indeks selulotik melaluli metode pewarnaan merah kongo pada media padat CMC 1%. Sebanyak tujuh isolat yang menandakan hasil positif dan nilai indeks tertinggi dihasilkan oleh isolat SI-E yaitu 4.7 mm. Setelah itu nilai aktivitas enzim selulase dari ketujuh isolat positif ditentukan dengan menghitung gula pereduksi substrat melalui metode 3,5-dinitrosalisilat (DNS). Hasil menunjukkan isolat SI-H memiliki aktivitas tertinggi yaitu 0,071 U/mL.

Co-Authors Aris Tri Wahyudi Darma Santi Elda Irma Jeanne Joice Kawulur Huliselan, Rendy Leonard Maria Massora Muhammad Dailami, Muhammad MUNTI YUHANA Murtihapsari . Rina A. Mogea Salosa, Yenni Yendri Tati Tati Tirza Bandi Waode Intan Cendrasari La Halim Putri Yenni Y Salosa

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