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All Journal WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences JURNAL BIOLOGI INDONESIA Jurnal Kedokteran Hewan
R.M. Abdul Adjid
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Infectious Bovine Rhinotracheitis (Ibr) on Cattle in Indonesia and The Strategy For Disease Control Adjid, R.M. Abdul; Saepulloh, M
Indonesian Bulletin of Animal and Veterinary Sciences Vol 20, No 1 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.762 KB) | DOI: 10.14334/wartazoa.v20i1.943

Abstract

Infectious Bovine Rhinotracheitis (IBR) caused by Bovine herpesvirus-1 (BHV-1) infects cattle and widely spreads in Indonesia. The disease infected cattle in breeding centers, artificial insemination centers and also holderfarmers. This infectious disease may cause economical losses primarily due to reproductive failure of infected animals. Recommended strategy for disease control is step by step control with priorities, started from upper to downstream, from breeding and artificial insemination (AI) centers as the first priority, then village breeding centers as the second priority, and the last priority is in cattle owned by smallholders. In the breeding and AI centers, eradication of the disease is carried out by surveilance, excluding reactors, and applying biosecurity. In the village breeding centers, the use of semen for AI should come from centers that free from IBR, the use of bull that free from IBR, surveilance and application of biosecurity. At the farmer levels, IBR control is bone by using semen from AI centers free from IBR and routine vaccination. The final step is performed after evaluating the successful rate and economic impact of the disease control. Key words: Cattle, IBR
Seroepidemiologi Nipah Virus pada Kalong dan Ternak Babi di Beberapa Wilayah di Indonesia Sendow, Indrawati; Field, Hume; Adjid, R.M. Abdul; Syafriati, Tatty; Darminto, Darminto; Morrissy, Chris; Daniels, Peter
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.164 KB) | DOI: 10.14203/jbi.v5i1.3205

Abstract

ABSTRACTNipah Virus Seroepidemiology in Flying Fox and Pig Husbandry in Several Areasof Indonesia. Nipah is a dangerous zoonotic disease which was carried by flying fox.The disease had been occurred in Malaysia in 1999 and infect pigs and caused humandeath. Indonesia is adjacent country to Malaysia, hence, a serological study had beenconducted on 156 flying fox (P. vampyrus) sera from North Sumatera, West Java, CentralJava and East Java. Besides that, 2740 pig sera was randomly collected in differentprovinces to detect Nipah infection. Both flying fox and pig sera were tested usingELISA test to detect the presence of Nipah antibody. The results indicated that 37 from156 flying fox sera (23.7%) has antibodies against Nipah virus. Infections were occuredin all sampling sites with the prevalence varied from 18% to 33 %. Meanwhile, no pigsera tested (2740) had antibody against Nipah virus. Based on these results it can beconcluded that Nipah virus infections were occurred in flying fox in some parts inIndonesia, but not in pigs. It was suggested that the presence of Nipah virus in Indonesiashould be anticipated. Hence the distribution of its infection in pigs and human must beanticipated. Monitoring of Nipah infection in areas adjacent to Malaysia must be increasedto detect the entering of the disease in Indonesia.Keywords: Nipah, pigs, flying fox, serology
SEROEPIDEMIOLOGI NIPAH VIRUS PADA KALONG DAN TERNAK BABI DI BEBERAPA WILAYAH DI INDONESIA Sendow, Indrawati; Field, Hume; Adjid, R.M. Abdul; Syafriati, Tatty; Darminto, Darminto; Morrissy, Chris; Daniels, Peter
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i1.3205

Abstract

ABSTRACTNipah Virus Seroepidemiology in Flying Fox and Pig Husbandry in Several Areasof Indonesia. Nipah is a dangerous zoonotic disease which was carried by flying fox.The disease had been occurred in Malaysia in 1999 and infect pigs and caused humandeath. Indonesia is adjacent country to Malaysia, hence, a serological study had beenconducted on 156 flying fox (P. vampyrus) sera from North Sumatera, West Java, CentralJava and East Java. Besides that, 2740 pig sera was randomly collected in differentprovinces to detect Nipah infection. Both flying fox and pig sera were tested usingELISA test to detect the presence of Nipah antibody. The results indicated that 37 from156 flying fox sera (23.7%) has antibodies against Nipah virus. Infections were occuredin all sampling sites with the prevalence varied from 18% to 33 %. Meanwhile, no pigsera tested (2740) had antibody against Nipah virus. Based on these results it can beconcluded that Nipah virus infections were occurred in flying fox in some parts inIndonesia, but not in pigs. It was suggested that the presence of Nipah virus in Indonesiashould be anticipated. Hence the distribution of its infection in pigs and human must beanticipated. Monitoring of Nipah infection in areas adjacent to Malaysia must be increasedto detect the entering of the disease in Indonesia.Keywords: Nipah, pigs, flying fox, serology
Infectious Bovine Rhinotracheitis (Ibr) on Cattle in Indonesia and The Strategy For Disease Control R.M. Abdul Adjid; M Saepulloh
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 20, No 1 (2010): MARCH 2010
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.762 KB) | DOI: 10.14334/wartazoa.v20i1.943

Abstract

Infectious Bovine Rhinotracheitis (IBR) caused by Bovine herpesvirus-1 (BHV-1) infects cattle and widely spreads in Indonesia. The disease infected cattle in breeding centers, artificial insemination centers and also holderfarmers. This infectious disease may cause economical losses primarily due to reproductive failure of infected animals. Recommended strategy for disease control is step by step control with priorities, started from upper to downstream, from breeding and artificial insemination (AI) centers as the first priority, then village breeding centers as the second priority, and the last priority is in cattle owned by smallholders. In the breeding and AI centers, eradication of the disease is carried out by surveilance, excluding reactors, and applying biosecurity. In the village breeding centers, the use of semen for AI should come from centers that free from IBR, the use of bull that free from IBR, surveilance and application of biosecurity. At the farmer levels, IBR control is bone by using semen from AI centers free from IBR and routine vaccination. The final step is performed after evaluating the successful rate and economic impact of the disease control. Key words: Cattle, IBR
PENGEMBANGAN TEKNIK ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) MENGGUNAKAN ANTIBODI MONOKLONAL UNTUK MENDETEKSI ANTIBODI PENYAKIT BOVINE EPHEMERAL FEVER Indrawati Sendow; R.M. Abdul Adjid; Atik Ratnawati; Muharam Saepulloh
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.824 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2775

Abstract

Penelitian ini bertujuan mengembangkan teknik enzyme-linked immunosorbent assay (ELISA) untuk mendeteksi antibodi terhadap virus bovine ephemeral fever (BEF). Pada penelitian ini dikembangkan uji ELISA langsung (direct ELISA) dan tidak langsung (indirect ELISA) dengan menggunakan antibodi monoklonal (blocking ELISA). Hasil penelitian menunjukkan bahwa uji direct ELISA tidak dapat digunakan dengan baik karena terjadi positif palsu. Uji blocking ELISA bereaksi lebih baik dan dapat dikembangkan lebih lanjut untuk mendeteksi antibodi terhadap penyakit BEF. Dapat disimpulkan bahwa pengembangan teknik deteksi dini terhadap BEF dengan mempergunakan antibodi monoklonal dapat diterapkan dalam upaya pengawasan penyakit dan surveilans.
PENGEMBANGAN TEKNIK ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) MENGGUNAKAN ANTIBODI MONOKLONAL UNTUK MENDETEKSI ANTIBODI PENYAKIT BOVINE EPHEMERAL FEVER Indrawati Sendow; R.M. Abdul Adjid; Atik Ratnawati; Muharam Saepulloh
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2775

Abstract

Penelitian ini bertujuan mengembangkan teknik enzyme-linked immunosorbent assay (ELISA) untuk mendeteksi antibodi terhadap virus bovine ephemeral fever (BEF). Pada penelitian ini dikembangkan uji ELISA langsung (direct ELISA) dan tidak langsung (indirect ELISA) dengan menggunakan antibodi monoklonal (blocking ELISA). Hasil penelitian menunjukkan bahwa uji direct ELISA tidak dapat digunakan dengan baik karena terjadi positif palsu. Uji blocking ELISA bereaksi lebih baik dan dapat dikembangkan lebih lanjut untuk mendeteksi antibodi terhadap penyakit BEF. Dapat disimpulkan bahwa pengembangan teknik deteksi dini terhadap BEF dengan mempergunakan antibodi monoklonal dapat diterapkan dalam upaya pengawasan penyakit dan surveilans.
Co-Authors Atik Ratnawati Daniels, Peter Daniels, Peter Darminto . Field, Hume Field, Hume Indrawati Sendow Indrawati Sendow M Saepulloh Morrissy, Chris Morrissy, Chris Muharam Saepulloh Tatty Syafriati