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Journal : BERITA BIOLOGI

PROSES NITRIFIKASI OLEH KULTUR MIKROBA PENITRIFIKASI N-Sw DAN ZEOLIT Agustiyani, Dwi; Imamuddin, Hartati; Gunawan, Edi; Darusman, Latifah K
BERITA BIOLOGI Vol 8, No 5 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.491 KB) | DOI: 10.14203/beritabiologi.v8i5.1904

Abstract

The addition of zeolite into the mix culture of nitrifying bacteria N-Sw was investigated in order to improve the nitrification activity. In this experiment, the ammonium conversion was investigated by zeolite as a sole agent and also mixed with nitrifying culture N-Sw.The mix culture of nitrifying bacteria N-Sw was developed from the sludge of wastewater treatment of palm oil industry, which acclimated by ammonium sulfate for about one year. The result show that the nitrification efficiency on the treatment using nitrifying culture N-Sw was 30.76%, the ammonium elimination rate was 7.46 mg N-NH /L/hour.The addition of 10 g/l zeolite, increase both the nitrification efficiency (64.58%), and the ammonium elimination rate (14.0 mg N-NH /L/hour).The nitrification efficiency increased to be 100% on the second and third day operation, and the ammonium elimination rate was increased to be 22.4–22.9 mg N-NH /L/hour. From this experiment indicated that the role of zeolite on the improving the nitrification activity was as an absorbent of ammonium.
BIODEGRADASI SENYAWA ORGANIK DAN AMONIUM DALAM LIMBAH CAIR INDUSTRI MINYAK KELAPA SAWIT DENGAN SISTEM PERTUMBUHAN MIKROORGANISMA TERLEKAT Agustiyani, Dwi; Imamuddin, Hartati
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (440.21 KB) | DOI: 10.14203/beritabiologi.v5i2.1148

Abstract

Efficiency of biodegradation of organic carbon and ammonium of sawit palm oil industry wastewater was investigated by using batch reactor with fluidized porous support particles.Two bioreactors, each had an effective volume of 3.0 L, were operated in parallel. Bioreactor-l is a batch reactor with fluidized porous support particles and bioreactor-ll is a suspended growth reactor.The wastewater consisting of 1500-2000 mg/L COD and 50-60 mg/L nitrogen was fed by fill and draw mode with one cycle per day,where 1.5 L treated water was drawn before adding the new wastewater.Efficiency of COD degradation and nitrification were calculated by measuring concentration of COD, ammonium-N, nitrite-N and nitrate-N.The experimental results showed that efficiency of COD degradation and nitrification tend to be higher in the bioreactor-l than in the bioreactor II.The highest efficiency of COD degradation in the reactor-l was 83.7% and in the bioreactor-ll was 63.44 %.The highest efficiency of nitrification in the bioreactor-l and bioreactor-ll was 76.72% and 56%, respectively.Ammonium removal occurred in the bioreactor might not only by biological nitrification, but also by other phisical or chemical processes.
FISIOLOGI PERTUMBUHAN, POTENSI AKTIFITAS PRODUKSI N2O DAN GEN FUNGSIONAL PENYANDINYA PADA BEBERAPA ISOLAT BAKTERI DENITRIFIKASI Agustiyani, Dwi; Laili, Nur; Antonius, Sarjiya
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4963.084 KB) | DOI: 10.14203/beritabiologi.v17i1.2340

Abstract

Physiological characters of four denitrifying bacteria (Bacillus sp. CPNS, Bacillus thuringiensis UPT1, Brevundimonas diminuta EA1 and Bacillus sp. UPSB) were studied based on the growth ability on various nitrate concentrations and the production of N2O gas. The characters of denitrifying bacteria were also evaluated through the existence of functional genes nirS and nosZ, encoding the nitrite reduction and nitrous oxide reduction enzymes which have important role on denitrification processes. The study showed that Bacillus sp. UPSB and Bacillus sp. CPNS isolates have a linear growth with the increasing concentration of KNO3. The N2O gas production of Bacillus sp. UPSB isolate was relatively high, about 70 ?/l, Bacillus sp. CPNS isolate was 25?/l, while the Bacillus thuringiensis UPT1 isolate was 5 ?/l and Brevundimonas diminuta EA1 isolate was 8 ?/l. It was also indicated that both Bacillus sp. UPSB and Bacillus sp. CPNS had high deninitrification activities. It was confirmed that all isolates were contained functional gen of nirS and nosZ.
NITROGEN REMOVAL BYAN ACTIVATED SLUDGE PROCESS WITH CROSS-FLOW FILTRATION [Perombakan Nitrogen Menggunakan Proses Lumpur Aktif yang Dilengkapi dengan Filtrasi] Agustiyani, Dwi; Yamagishi, Takao
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1075.241 KB) | DOI: 10.14203/beritabiologi.v10i1.2049

Abstract

The simultaneous nitrification and denitrification in a single reactor using an activated sludge with cross-flow filtration was investigated.The reactor was a Sequencing Batch Reactor (SBR), operated on three phase conditions, phase I was continuous aeration, phase II was intermittent aeration and phase III was intermittent aeration with methanol addition. The microbial properties on the nitrogen removal processes were monitored by measuring the ammonia decreasing and nitrate production rate.The denitrification rate was also calculated from N20 gas production by acetylene inhibition method.The experiment results show that the nitrification was occurred during the aeration condition, both in the phase I and II. The denitrification was occurred in the anoxic stage (phase III).The nitrate decreasing rate in the anoxic stage (phase IIIA) increased gradually reach the value of 0.19 th mg-N/1/min. on the 8 day operation. The nitrate decreasing rate increased to be 0.45 mg/l/min in the phase IIIB and reach up to 0.70 mg/l/min in the phase IIIC in which the methanol concentrations was increased from 762 mg/L to 1016 mg/L in the phase IIIB and phase IIIC, respectively. The increasing activity of denitrification resulted in decreasing the concentration of TOC in the reactor. More than 80% nitrogen removal occurred in phase III and TOC removal efficiency in phase III reach more than 90%.