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All Journal The Indonesian Biomedical Journal Cermin Dunia Kedokteran
Purba, Jan Sudir
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Public Health

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Potensi Terapi Sel Punca untuk Penyakit Alzheimer: Kenyataan atau Harapan? Purba, Jan Sudir
Cermin Dunia Kedokteran Vol 47, No 1 (2020): Bedah
Publisher : PT. Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.208 KB) | DOI: 10.55175/cdk.v47i1.344

Abstract

Penyakit Alzheimer (AD) adalah penyakit neurodegeneratif menyangkut penurunan kemampuan fungsi otak yang menyebabkan gangguan perilaku serta kognitif yang progresif. Gangguan visuospasial juga sering ditemukan. Faktor risiko terbesar yang diketahui adalah bertambahnya usia dengan mayoritas 65 tahun ke atas. Perkembangan ilmu kedokteran akhir-akhir ini memungkinkan terapi sel punca pada penyakit neurodegenetatif. Dalam tulisan ini dibahas kemungkinan terapi sel punca pada penyakit Alzheimer.Alzheimer disease (AD) is a long-term and progressive neurodegenerative disorder that leads to a disability of performing simple daily tasks, often accompanied by visual disturbances. Symptoms are progressively deteriorates with age. Current therapies only target the relief of symptoms using psychotherapy and various drugs, and do not cure the disease. Stem cell therapy holds a great promise and provides a great research opportunity. Recently, stem cell therapy has been shown to be a potential approach to various diseases, including neurodegenerative disorders. In this review, we focus on stem cell therapies for AD
Penyakit Autoimun dan Terapi Herbal: Peran Nanoteknologi terhadap Efektivitas Obat Herbal Purba, Jan Sudir
Cermin Dunia Kedokteran Vol 46, No 3 (2019): Nutrisi
Publisher : PT. Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (526.392 KB) | DOI: 10.55175/cdk.v46i3.511

Abstract

Penyakit autoimun adalah penyakit akibat kesalahan pengenalan sistem imun dalam tubuh sehingga sel yang sewajarnya dilindungi malah dianggap musuh. Salah satu yang termasuk dalam kelompok penyakit autoimun adalah Myasthenia gravis (MG). Obat kortikosteroid seperti prednisolon, obat imunosupresan seperti azathioprine, bisa digunakan untuk membantu menekan respon imun tubuh yang berlebihan. Kholinesterase inhibitor, seperti pyridostigmine, dapat memperbaiki komunikasi antara sel saraf dan otot. Penggunaan jangka panjang obat-obat di atas dapat menimbulkan masalah yang berkaitan dengan efikasi, dosis serta efek samping dan biaya pengobatan. Pada beberapa penelitian hewan coba, ekstrak herbal Acalypha indica Linn (AI) memperlihatkan efek hampir sama dengan pyridostigmine pada MG. Untuk meningkatkan efikasinya, ekstrak AI ini perlu diproses melalui pengembangan nano teknologi. Penggunaan sediaan dalam bentuk nano diharapkan dapat meningkatkan efek terapeutik dan meminimalkan dampak negatif serta pembiayaan.Autoimmune disease is an illness in which the immune system produce antibodies that attack normal body tissues. Myasthenia gravis (MG) is an autoimmune disease of the neuromuscular junction (NMJ). Corticosteroid drugs such as prednisolone, and immunosuppressant drugs such as azathioprine, can be used to help suppress the body's excessive immune response. Cholinesterase inhibitors, such as pyridostigmine, can improve communication between nerve cells and muscles, but long-term use can cause problems related to efficacy, doses, side effects and medical costs. Studies in experimental animals show the effect of Acalypha indica Linn (AI) extract is almost the same as pyridostigmine. In the future, research needs to be done to improve the efficacy of this drug through the development of nano technology. 
The Injected Plasma of Myasthenia Gravis Patient with A Low T-reg Level Caused Clinical Myasthenic Syndromes in Swiss-Webster Mice Pasmanasari, Elta Diah; Purwaningsih, Erni Hernawati; Retnaningsih, Retnaningsih; Purba, Jan Sudir; Octaviana, Fitri
The Indonesian Biomedical Journal Vol 16, No 4 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i4.3086

Abstract

BACKGROUND: Myasthenia gravis (MG) is a rare autoimmune disease affecting neuromuscular junction involvement. The finding that T-reg level in MG patients was lower than that in normal persons leads to the idea that the primary pathology of the disease is T-reg dependent. The T-reg level of MG patients seems to be decreasing compared to that of normal persons. The study was conducted to observe the contribution of T-reg level in plasma injected into Swiss-Webster mice to develop clinically and pathologically myasthenic syndromes.METHODS: Swiss-Webster mice were grouped into three groups: the groups received plasma with normal, low, and high T-reg levels, respectively. The T-reg levels of the mice were measured with flow cytometry analysis and a human regulatory T-cell cocktail for T-cell surface cell marker. The motor function, interleukin (IL)-2, interferon (IFN)-γ, and thymus weight of mice were measured after the injection. Histopathological examination was performed to analyze mice’s muscles and thymus.RESULTS: The result identified that the motor function (2-week treatment group: p=0.021 and 3-week treatment group: p=0.032) and muscle width (p=0.014, p=0.032 and p≤0.001) were significantly lower in the low T-reg level plasma group compared to control and high T-reg level plasma groups. The thymus showed an increase in weight without an increase in the cortex-medulla ratio of the thymus, indicating hyperplasia. Both IL-2 and IFN-γ levels were lower in the low and high T-reg level groups compared with the control group, indicating the autoimmune process.CONCLUSION: Low T-reg level was associated with lower motor function, muscle width, increased thymus weight, as well as lower IL-2 and IFN-γ levels. T-reg level contributed to clinical myasthenic syndromes but not pathological findings. This research method is expected to be a basis for the development of animal models with Swiss-Webster mice.KEYWORDS: animal model, Myasthenia gravis, Swiss-Webster mice
Co-Authors Erni Hernawati Purwaningsih, Erni Hernawati Fitri Octaviana Pasmanasari, Elta Diah Retnaningsih Retnaningsih