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All Journal The Indonesian Biomedical Journal Paediatrica Indonesiana JSFK (Jurnal Sains Farmasi & Klinis) JIMKI: Jurnal Ilmiah Mahasiswa Kedokteran Indonesia Jurnal Info Kesehatan
Erni Hernawati Purwaningsih, Erni Hernawati
Department Of Medical Pharmacy, Faculty Of Medicine Universitas Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Duration of watching TV and child language development in young children Silva Audya Perdana; Bernie Endyarni Medise; Erni Hernawati Purwaningsih
Paediatrica Indonesiana Vol 57 No 2 (2017): March 2017
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.599 KB) | DOI: 10.14238/pi57.2.2017.99-103

Abstract

Background Many factors contribute to language development in children. About 5-8% of children in Indonesia experience delayed language skills. Young children need appropriate stimulation for optimal development. Children who watch television (TV) for long periods of time may receive less two-way interaction, the appropriate stimulation for learning. As such, shorter duration of the appropriate stimulation may impede language development in small children.Objective To assess for an association between duration of watching TV and language development in young children.Methods This cross-sectional study was done with primary data collected from questionnaires. Subjects, aged 18 months to 3 years, were from a Jakarta-area community health center (Puskesmas) Jatinegara and the Pediatric Growth and Development Clinic, Cipto Mangunkusumo Hospital, Jakarta. Their language development was tested using the Developmental Pre-screening Questionnaire (Kuesioner Pra Skrining Perkembangan, KPSP) and the Early Language Milestone (ELM Scale 2) test.Results From a total of 84 subjects, 47 (56%) had normal and 37 (44%) had delayed language development. Duration of watching TV was categorized as <4 hours per day or >4 hours per day. Children who watched TV >4 hours/day (OR 4.4; 95%CI 1.68 to 11.7; P=0.002), and children who watched both Indonesian and English language TV programs (OR 14.7; 95%CI 1.77 to 123.0; P=0.004) had higher risk of language delay. Other variables such as sex, first age exposed to TV, use of gadgets, and TV in the bedroom had no significant associations with delayed language development.Conclusion Children who watch TV >4 hours/day had four times higher risk of developing language delay. In addition, those who watch TV programs in both Indonesian and English, also have a 14.7 higher risk of delayed language development.
Perubahan Parameter Biokimia, Histopatologi Ginjal Tikus Spraque Dawley Pascahipoksia Oleh Ekstrak Akar Acalypha indica dan Herba Centella asiatica Nurfitri Nurfitri; Erni Hernawati Purwaningsih; Vivian Soetikno; Adisti Dwijayanti; Novi Silvia Hardiany
Jurnal Sains Farmasi & Klinis Vol 5, No 3 (2018): J Sains Farm Klin 5(3), Desember 2018
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (731.281 KB) | DOI: 10.25077/jsfk.5.3.160-168.2018

Abstract

Hipoksia kronik merupakan salah satu penyebab penyakit ginjal akibat peningkatan pembentukan Reactive Oxygen Species (ROS)  dalam sel. Kombinasi ekstrak akar Acalypha indica 250 mg/KgBB (AI250) dan Centella asiatica 150 mg/kgBB (CA150) memiliki efek neuroterapi pada tikus Spraque Dawley pascahipoksia. Penelitian dilakukan untuk membuktikan manfaat kombinasi ekstrak etanol dan/atau ekstrak tunggalnya dapat memperbaiki kerusakan ginjal tikus pascahipoksia melalui mekanisme antioksidan. 28 tikus jantan dikelompokkan dalam 7 kelompok: kontrol normal; kontrol hipoksia+air; hipoksia+(AI200+CA150); hipoksia+(AI250+CA100); hipoksia+AI250; hipoksia+CA150; hipoksia+vitamin C. Hipoksia selama 7 hari dalam hypoxic chamber berisi O2 10% dan N2 90%, 1 atm. Setiap kelompok diberi perlakuan selama 7 hari. Pada akhir studi hewan diterminasi. Darah dan organ ginjal diambil untuk pemeriksaan biokimia dan histopatologi.Kombinasi  (AI250+CA100) menurunkan kadar MDA ginjal dan plasma secara bermakna dibandingkan kontrol hipoksia (p=0,001 dan p=0,021) dan AI250 (p=0,003 dan 0,043). Kombinasi AI250+CA100 terjadi penurunan ekspresi relatif mRNA HIF-1α (p=0,014), kadar urea plasma (p=0,001) dan perbaikan lesi intra-glomerulus p=0,013.Kesimpulan: Kombinasi (AI250+CA100) dan tunggal AI250 memiliki aktivitas antioksidan terbaik dalam mencegah kerusakan ginjal pascahipoksia, secara biokimiawi dan histopatologinya.
Chelating Effect of Water Extract of Mangifera foetida L. Leaf in Serum of Thalassemia Patient by Ex Vivo Test Dessy Framita Sari; Erni Hernawati Purwaningsih; Desak Gede Budi Krisnamurti
JIMKI: Jurnal Ilmiah Mahasiswa Kedokteran Indonesia Vol 1 No 2 (2012): JIMKI : Jurnal Ilmiah Mahasiswa Kedokteran Indonesia
Publisher : BAPIN-ISMKI (Badan Analisis Pengembangan Ilmiah Nasional - Ikatan Senat Mahasiswa Kedokteran Indonesia)

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Abstract

Thalassemia is a genetic disorder, which is caused by the diminshed synthesis of globin polypeptide chains. In Indonesia, 3-5% of cases are beta-thalassemia and 2.6 to 11% are alpha-thalassemia. Regular blood transfusion is neede but iron overload is the consequence. That's why Deferoxamine is used as a chelating agent which function is bind iron and excrete them. Unfortunately, Deferoxamine needs high cost with high side effects. Therefore, an alternative natural medicine is required, that is mangiferin derived from aqueous extract of Mangifera foetida L. leaf. The aim of this research is to utilize the natural substance as a chelating agent of feritin in thalassemia patient's serum. This was an experimental study, which used serum of patient with thalassemia. One Way ANOVA statistical test proved that the aqueous ectract of Mangifera foetida L. dose of 0.375 mg and 0.75 mg has a chelating effect compared with negative control (p=0.005). However, when subsequently tested with post hoc, 0.375 mg extract doesn't show a chelating effect compared with mangiferin (p=0.018). In the other hand, 0.75 mg extract has shown a chelating effect but not as good as mangiferin (p=0.259). It is consiered that the low doses of extract and a possibility that the extract doesn't bind the iron directly are the facttors which influence the result. That's why manfigerin still has better effectiveness in binding iron compared with aqueous extract of Mangifera foetida L. leaf dose of 0.375 mg and 0.75 mg.
The Injected Plasma of Myasthenia Gravis Patient with A Low T-reg Level Caused Clinical Myasthenic Syndromes in Swiss-Webster Mice Pasmanasari, Elta Diah; Purwaningsih, Erni Hernawati; Retnaningsih, Retnaningsih; Purba, Jan Sudir; Octaviana, Fitri
The Indonesian Biomedical Journal Vol 16, No 4 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i4.3086

Abstract

BACKGROUND: Myasthenia gravis (MG) is a rare autoimmune disease affecting neuromuscular junction involvement. The finding that T-reg level in MG patients was lower than that in normal persons leads to the idea that the primary pathology of the disease is T-reg dependent. The T-reg level of MG patients seems to be decreasing compared to that of normal persons. The study was conducted to observe the contribution of T-reg level in plasma injected into Swiss-Webster mice to develop clinically and pathologically myasthenic syndromes.METHODS: Swiss-Webster mice were grouped into three groups: the groups received plasma with normal, low, and high T-reg levels, respectively. The T-reg levels of the mice were measured with flow cytometry analysis and a human regulatory T-cell cocktail for T-cell surface cell marker. The motor function, interleukin (IL)-2, interferon (IFN)-γ, and thymus weight of mice were measured after the injection. Histopathological examination was performed to analyze mice’s muscles and thymus.RESULTS: The result identified that the motor function (2-week treatment group: p=0.021 and 3-week treatment group: p=0.032) and muscle width (p=0.014, p=0.032 and p≤0.001) were significantly lower in the low T-reg level plasma group compared to control and high T-reg level plasma groups. The thymus showed an increase in weight without an increase in the cortex-medulla ratio of the thymus, indicating hyperplasia. Both IL-2 and IFN-γ levels were lower in the low and high T-reg level groups compared with the control group, indicating the autoimmune process.CONCLUSION: Low T-reg level was associated with lower motor function, muscle width, increased thymus weight, as well as lower IL-2 and IFN-γ levels. T-reg level contributed to clinical myasthenic syndromes but not pathological findings. This research method is expected to be a basis for the development of animal models with Swiss-Webster mice.KEYWORDS: animal model, Myasthenia gravis, Swiss-Webster mice
Moringa oleifera as Anticancer: A Review of Recent Studies Kambuno, Norma Tiku; Purwaningsih, Erni Hernawati
JURNAL INFO KESEHATAN Vol 22 No 2 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss2.1515

Abstract

Moringa oleifera Lam (MO) plants have long been reported to have many pharmacotherapy benefits. In vitro and in vivo studies have shown that MO extracts have various biological activities and therapeutic effects, including cardioprotective, cardiometabolic, hypocholesterolemic, neuroprotective, anti-inflammatory, antioxidant, anti-hypertensive, anti-diabetic, anti-bacterial, immunomodulatory and anticancer. Researchers have tested extracts from various parts of the MO tree, both in vitro and in vivo, on several types of cancer (such as liver cancer cells, breast cancer, colorectal, leukemia, lung cancer, and oral cancer) with varying success. This review aims to explore the current state of the latest anticancer activity research of MO plants in the last five years. We tried to explore the anticancer activities of MO extracts from reported in vivo and in vitro studies. We searched systematically from three databases (PubMed, Scopus, and Embase) and summarized the data. The keywords used were “Moringa oleifera” AND “anticancer” AND “in vivo” OR “in vitro”. The inclusion criteria were in vivo or in vitro experimental studies and exclusion criteria analyses i.e., in silico trials, study protocols, reviews, or observational studies. This review includes 16 papers on nonclinical studies of MO anticancer activity. Several active compounds have been purified and have reported their anticancer effectiveness, including glucomoringin-ITC/MIC-1, 7-octanoic acid, oleamide, 1-phenyl-2-pentanol, quercetin, gallic acid, p-coumaric acid, and 4-hydroxy 3 – methoxy cinnamic acid, quinic acid. There was no difference in the mechanism of anticancer action based on plant parts, leaves, roots, and seeds, even though using different extraction methods. The general mechanism of action shown was apoptotic, antiproliferative, and cytotoxic. The dose used differed depending on the type of cancer cells used. Some used conventional extraction methods, and others have used modern techniques to extract the purified active compounds from the fractionation process. Our review made it clear that MO could be an excellent and safe candidate for the development of novel therapies against cancer and was most commonly reported in MCF-7, HepG2, and HCT-116 cancer cells. In addition, the development of MO products as future cancer prevention is also interesting to be explored and developed optimally in clinical settings.                         
Co-Authors Adisti Dwijayanti Ahmad Aulia Jusuf Arjo Tedjo Aryenti . Asri Rasad Bernie Endyarni Medise Desak Gede Budi Krisnamurti Dessy Framita Sari Fitri Octaviana Franciscus D. Suyatna Hamdani Zain Hans -Joachim Freisleben Hans Joachim Freisleben Hedi R. Dewoto Juniarti . Mohamad Sadikin Norma Tiku Kambuno Novi Silvia Hardiany Nurfitri Nurfitri Nurhadi Ibrahim Oentoeng Soeradi Pasmanasari, Elta Diah Purba, Jan Sudir Retnaningsih Retnaningsih Rolf Schubert Silva Audya Perdana Vivian Soetikno Wawaimuli Arozal Yuhernita .