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TNF-α -308 G/A Gene Polymorphism as a Risk Factor for Pulmonary Tuberculosis in Cirebon, Indonesia Oktaviyati, Nurfithria; Pratamawati, Tiar Masykuroh; Nauphar, Donny
GHMJ (Global Health Management Journal) Vol. 8 No. 1s (2025): Special Issues
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-81s1159

Background: Mycobacterium tuberculosis, the causative agent of pulmonary tuberculosis, spreads via droplets. The TNF-α-308 G/A gene polymorphism is one of the host genetic variables that may affect an individual's vulnerability to the disease. However, this polymorphism has not been studied in Cirebon. Aims: To analyze the TNF-α -308 G/A gene polymorphism as a risk factor for the occurrence of pulmonary tuberculosis in Cirebon. Methods: A total of 64 participants joined part in an analytical observational study using a case-control design at the Biomolecular and Genetics Laboratory, Faculty of Medicine, Universitas Swadaya Gunung Jati, Indonesia. DNA extraction from blood samples, ARMS-PCR genotyping, and 1.5% electrophoresis gel visualization were all part of the data gathering process. The chi-square test was used to analyze the data. This study including inclusion criteria, exclusion criteria, and sample control for the research. Results: According to the study, there is no link between Cirebon's risk of pulmonary tuberculosis and the polymorphism in the TNF-α-308 G/A gene (OR = 0.462; P > 0.05). However, the study shown a protective factors which means that individuals with the TNF-α -308 G/A gene polymorphism have a lower risk of developing pulmonary tuberculosis compared to those without the polymorphism. Conclusion: The TNF-α-308 G/A gene variant is not associated with an increased risk of pulmonary tuberculosis in the Cirebon community.

Potassium Inwardly Rectifying Channel Subfamily J Member 11 (KCNJ11) RS5219 Gene Polymorphism as a Risk Factor for Type 2 Diabetes Mellitus in Indonesia: A Case Control Study Putri, Annisa Septiani Putri; Pratamawati, Tiar Masykuroh; Nauphar, Donny
GHMJ (Global Health Management Journal) Vol. 8 No. 1s (2025): Special Issues
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-81s1160

Background: Diabetes Mellitus (DM) is a chronic metabolic disease caused by the failure of the pancreas to produce the hormone insulin or ineffective use of the hormone insulin. It is estimated that 537 million adults aged 20-79 years worldwide suffer from DM. Genetics is one of the risk factors involved in the pathophysiology of type 2 DM. The KCNJ11 gene encodes the Kir6.2 protein that is responsible for adenosine triphosphate-sensitive potassium ion channels (kATP) synthesis in pancreatic beta cells plasma membrane. Aims: This study aims to examine the KCNJ11 rs5219 gene polymorphism as a risk factor for type 2 diabetes mellitus in Cirebon population. Methods: This case control study involved 29 cases of type 2 diabetes mellitus and 29 healthy controls with purposive sampling technique. Sample data was obtained through the examination of blood sugar, DNA extraction, PCR-RFLP with Eco24I restriction enzyme, then visualization of the results with Gel Electrophoresis. Results: The frequency of G allele was found more in the case group (70%) while the frequency of A allele was found more in the control group (38%). The frequency of heterozygous GA genotype was found more in the control group (48.3%) and the frequency of homozygous mutant AA genotype was more in the case group (17.2%) compared to the control group (13.8%). Chi-Square Test results obtained p-value 0.115, OR value 2.318. Conclusion: This study showed no significant association between Potassium Inwardly Rectifying Channel Subfamily J Member 11 (KCNJ11) rs5219 gene polymorphism and the incidence of Type 2 Diabetes Mellitus in Cirebon population.

Angiotensin Converting Enzyme 2 (ACE2) G8790A Gene Polymorphism as a Risk Factor for Essential Hypertension Husna, Nazaul; Nauphar, Donny; Pratamawati, Tiar Masykuroh
GHMJ (Global Health Management Journal) Vol. 8 No. 2s (2025): Special Issues
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-82s1231

Background: Globally, the number of people with hypertension has doubled, from 650 million to 1.3 billion. The World Health Organization reported that hypertension is responsible for more than 10 million deaths every year. Essential hypertension is a multifactorial condition with genetics as one of the factors. Genome-Wide Association Study has identified several genes associated with hypertension, one of which is the Angiotensin Converting Enzyme 2 (ACE2) gene. Essential hypertension may be predisposed to by the G8790A polymorphism of the ACE2 gene, which is hypothesized to interfere with the normal function of the Renin Angiotensin System (RAS). Aims: The purpose of this study is to determine whether the ACE2 G8790A gene polymorphism in Cirebon, West Java, Indonesia, is associated with an increased risk of essential hypertension. Methods: This is a case-control study conducted at the Talun Health Center, Cirebon Regency, April-August 2024, involving 30 essential hypertensive patients and 30 healthy controls. The study population comprised adults aged 30 to 72 years. Data was obtained through the examination of blood pressure, DNA extraction, PCR-RFLP with ALUI restriction enzyme, and then visualization of the results with Gel Electrophoresis. The Chi-Square Test technique and the Odds Ratio (OR) computation were used to analyze the data. Results: The G allele was higher in the case group 33 (55%), while the A allele was higher in the control group 34 (56.7%). The statistical analysis showed that there was no significant link between the ACE2 G8790A gene variation and essential hypertension, with a p-value of 0.592 (p > 0.05) (OR = 0.750; CI = 0.262–2.151). Conclusion: The ACE2 gene G8790A polymorphism and the rate of hypertension in Cirebon, West Java, were not significantly correlated. Further research is required on a larger scale to investigate the effects of gene combinations or interactions with other locus genes on essential hypertension.

Genetic Polymorphism of Interferon-Gamma +874T/A as a Risk Factor for Pulmonary Tuberculosis in Cirebon, Indonesia Kiteswara, Allain Umadela Deuxawalu; Sari, Ariestya Indah Permata; Pratamawati, Tiar Masykuroh
GHMJ (Global Health Management Journal) Vol. 7 No. 3s (2024)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-741107

Background: Tuberculosis (TB) is one of the leading causes of death globally, caused by Mycobacterium tuberculosis. With 10% of all cases worldwide in 2022, Indonesia is the second-largest contributor of tuberculosis cases. IFN-γ gene polymorphism is one of the factors that have been studied extensively for its association with TB. Aims: To analyze IFN-γ +874T/A gene polymorphism as a risk factor for pulmonary tuberculosis in Cirebon. Methods: Observational analysis with case control design was used in this study. Thirty-two tuberculosis patients as cases and 32 healthy controls at RSUD Waled were collected and performed DNA extraction to evaluate the polymorphism by using Amplification-refractory mutation system–polymerase chain reaction (ARMS–PCR). Statistical comparison was performed by using Pearson Chi-square and Kruskal Wallis test. Mann-Whitney U test was done for post hoc test. Odds ratio was calculated to see the risk of the assessed variables, including genotype, allele frequency, and the presence of polymorphism. Results: In the case group, the frequency of TT genotype was 3 (9.4%), TA genotype was 26 (81.3%), AA genotype was 3 (9.4%). In the control group, the frequency of TT genotype was 12 (37.5%), TA genotype was 17 (53.1%), AA genotype was 3 (9.4%). A significant difference (p=0.034) was found among 3 genotype groups. Post hoc test revealed that TT and TA was the pair with significant difference (p=0.007). In addition, TA polymorphism was significantly associated (p=0.004) with tuberculosis (OR=6.614; CI95% = 1.660-26.349). Conclusion: IFN-γ +874 TA gene polymorphism is associated with pulmonary tuberculosis in the population of Cirebon, West Java, Indonesia. Received: 25 September 2024 | Reviewed: 23 October 2024 | Revised: 10 November 2024 | Accepted: 30 November 2024

Association of rs9939609 FTO Gene Polymorphism as a Risk Factor of Obesity in Adults Pratamawati, Tiar Masykuroh; Sadji, Angel Angriani Elma Budai; Brajadenta, Gara Samara; Nauphar, Donny
GHMJ (Global Health Management Journal) Vol. 7 No. 1 (2024)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-71967

Background: The cause of obesity is an imbalance between the number of calories taken and the amount burned. Obesity is a complex disease. The FTO rs9939609 gene polymorphism is one of the genetic factors that contribute to obesity in addition to environmental factors. Numerous researches have suggested a connection between the prevalence of obesity and the FTO rs9939609 gene polymorphism Aims: The purpose of this study is to ascertain how the FTO rs9939609 gene polymorphism relates to the prevalence of adult obesity. Methods: At the Biomolecular and Genetics Laboratory of the UGJ Faculty of Medicine, an analytical observational study using a case-control design was carried out with 84 participants, 42 subjects in case group, and 42 subjects in control groups. Data were collected utilizing DNA from blood collection, PCR-RFLP for genotyping, and 2.5% electrophoretic gel for visualization. Chi-square was used for data analysis. Results: Findings showed that there is no link between the FTO rs9939609 polymorphism and the prevalence of obesity (p>0.05, OR=0.710). Conclusion: In the Indonesian population, the FTO rs9939609 gene polymorphism is not associated with an increased risk of obesity.

Mothers’ parenting patterns on stunting cases in toddlers: A case from Cirebon Health Center, West Java, Indonesia Suhaeni, Eni; Azmii, Salman Nur; Ulfah, Siti Maria; Pratamawati, Tiar Masykuroh
GHMJ (Global Health Management Journal) Vol. 7 No. 2 (2024)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-72968

Background: A recent survey conducted in 2021 noted a high stunting incident was found in Cirebon, West Java of Indonesia, reached to 30.6%, even higher compare to the provincial rate (24.5%). It has been known that parenting patterns and nutritional status are greatly influenced by the mother's understanding of managing health and nutrition for their baby in order to lower the stunting case. Aims: This study is to analyze the association between mother's parenting patterns and stunting in toddlers age 24-48 month in Cirebon Municipality. Methods: A total of 68 mothers have been selected using the consecutive sampling from a health facility in Cirebon City, Indonesia. This research uses survey and descriptive methods with a quantitative approach to examine the impact of mother's parenting patterns on stunting incidence in toddlers aged 24-48 months. This research also uses secondary data in the form of KIA (Mother and Child Health) records to identify the stunting cases among the respondents. Statistical analysis was employed to find how significant the variables affecting to the others. Results: From the questionnaire, it was found that the majority of participants were mothers aged 26-35 years old, and only graduated from high school or lower, and most of them were not working mothers. The stunting incidence found in this health center reached to 58.8 %. While only 36.8% of mothers exhibits well parenting pattern, the majority may show sufficient level (52.9%). Even only 10.3% of respondents correspond with low parenting pattern, however all of them found with stunting incidence. From statistical analysis, it is noted that mothers’ parenting pattern significantly affecting stunting cases in toddlers (p value = 0.001). Conclusion: This recent study concludes that parenting pattern significantly contributes to the number of stunting cases in toddler aged 24-48 months. The statistical analysis found mothers with well parenting pattern lower chance of having a stunted child. The data highlights the importance of knowledge and attitude will benefit in reducing the total number of stunted children in Cirebon city, Indonesia. Keywords: Parenting pattern; Stunting; Mothers; Toddlers; Indonesia. Received: 09 November 2023, Reviewed: 19 November 2023, Revised: 06 January 2024, Accepted: 03 June 2024.

POLIMORFISME GEN ANGIOTENSIN II TYPE 1 RECEPTOR (A1166C) SEBAGAI FAKTOR RISIKO PADA PASIEN DENGAN HIPERTENSI ESENSIAL DI KABUPATEN CIREBON Pratamawati, Tiar Masykuroh; Akbarahma, Ikrama; Zein, Ahmad Fariz Malvi Zam Zam; Sulistiyana, Catur Setiya; Suhaeni, Eni; Nauphar, Donny
InaBHS (Indonesian Journal of Biomedicine and Health Science) Vol 4 No 1 (2025): Indonesian Journal of Biomedicine and Health Science
Publisher : Fakultas Kedokteran UGJ Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33603/inabhs.v4i1.10797

Abstrak Latar Belakang: Hipertensi merupakan salah satu penyakit dengan prevalensi tertinggi, termasuk di Kabupaten Cirebon. Salah satu penyebab hipertensi esensial adalah pengaruh genetik. Mutasi pada gen yang berhubungan dengan hipertensi dapat memengaruhi sistem renin-angiotensin dalam mengatur tekanan darah. Polimorfisme gen AGTR1 (A1166C) terkait dengan peningkatan aktivitas angiotensin II dan berhubungan dengan hipertensi esensial. Tujuan: Penelitian ini bertujuan untuk mengetahui hubungan polimorfisme gen AGTR1 (A1166C) sebagai faktor risiko pada pasien hipertensi esensial di Kabupaten Cirebon. Metode: Penelitian ini dilakukan dengan metode observasional analitik dengan pendekatan kasus-kontrol dan melibatkan 34 pasien hipertensi dan 34 kontrol sehat. PCR-RFLP digunakan untuk memeriksa polimorfisme. Analisis data dilakukan dengan chi square test dan odds ratio. Hasil: Analisis data menunjukkan tidak ada hubungan yang signifikan pada polimorfisme gen AGTR1 (A1166C) dengan hipertensi esensial (p=0,050), namun hasil perhitungan odds ratio menunjukkan bahwa polimorfisme gen AGTR1 (A1166C) memiliki peluang 3,164 kali lebih besar berisiko untuk memiliki hipertensi esensial (OR=3,164). Simpulan: Tidak terdapat hubungan yang signifikan antara polimorfisme gen AGTR1 (A1166C) dengan kejadian hipertensi esensial. Kata Kunci: polimorfisme gen AGTR1 A1166C, Hipertensi Abstract Background: Hypertension is a disease with a fairly high prevalence, including in Cirebon Regency. One of the causes of essential hypertension is genetic influence. Mutations in genes associated with hypertension can affect the renin-angiotensin system in regulating blood pressure. AGTR1 Gen Polymorphism (A1166C) is related to increased angiotensin II activity and associated with essential hypertension. Aim: This study aims to determine the relationship between AGTR1 (A1166C) gene polymorphism as a risk factor in essential hypertension patients in Cirebon Regency. Methods: This study was conducted using an analytical observational method with a case-control approach and involved 34 hypertensive patients and 34 healthy controls. PCR-RFLP was used to check for polymorphisms. Data analysis was carried out with chi square test and odds ratio. Result: Data analysis showed that there was no significant relationship between the AGTR1 gene polymorphism (A1166C) and essential hypertension (p=0.050), however the odds ratio calculation results showed that the AGTR1 (A1166C) gene polymorphism had a 3.164 times greater chance of having essential hypertension (OR =3.164). Conclusion: There is no significant relationship between the AGTR1 gene polymorphism (A1166C) and the incidence of essential hypertension. Keywords: AGTR1 gene A1166C polymorphism, Hypertension

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