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Sari, Ariestya Indah Permata
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The International Conferences on Applied Science and Health (ICASH): Efforts and Commitments in Translating Research Results into Policy and Practices, for a Better Society Widyastari, Dyah Anantalia; Sinaga, Doni Marisi; Wibowo, Canggih Puspo; Rahman, Pamuko Aditya; Noor, Hafizh Muhammad; Irawan, Dimas Sondang; Fatmarizka, Tiara; Diary, Dwina Gustia; Putra, I Gusti Ngurah Edi; Maretalinia, Maretalinia; Viniriani, Marina Ayu; Destiwi, Monita; Shidiq, Galih Albarra; Pratiwi, Ivana Aprilia; Kusuma, Made Dian Shanti; Dharmapatni, Ni Wayan Kesari; Pratama, Mahendrawan Nugraha Aji; Norsaputra, Ary; Pancanugraha, Andi; Kurniawan, Febrian Hendra; Putra, Wahyu Manggala; Fattah, Rifqi Abdul; Moehas, Puput Kusumawardani; Samosir, Ricky Alexander; Hananto, Andang Adi Fitria; Agastya, I Gusti Ngurah; Susilo, Taufik Eko; Vejvisithsakul, Pichpisith Pierre; Nauphar, Donny; Sari, Ariestya Indah Permata; Macnab, Andrew John
GHMJ (Global Health Management Journal) Vol 3, No 3 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (787.653 KB) | DOI: 10.35898/ghmj-33451

Abstract

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Angiotensin-Converting Enzyme Insertion/Deletion (ACE I/D) Gene Polymorphism as a Risk Factor for Essential Hypertension Kuswara, Hasna Nurazizah; Nauphar, Donny; Sari, Ariestya Indah Permata
GHMJ (Global Health Management Journal) Vol. 7 No. 3s (2024)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-741044

Abstract

Background: Hypertension is the leading cause of death globally due to its complications, including coronary heart disease and stroke. In 2018, hypertension cases in West Java were the second highest among all populations in Indonesia. Genetics is one of the unmodifiable risk factors for hypertension. Angiotensin-converting enzyme insertion/deletion (ACE I/D) gene polymorphism could affect ACE production in the renin-angiotensin-aldosterone system (RAAS), which is linked to the regulation of blood pressure. Aims: To analyze ACE I/D gene polymorphism as a risk factor for hypertension in Cirebon. Methods: An observational analysis with a case-control design was used in this study. Blood samples were collected from 30 hypertensive patients and 30 healthy individuals at Talun Health Center. DNA extraction was performed to evaluate polymorphisms using ARMS-PCR. Statistical analyses, including the Chi-square test, Fisher’s exact test, Mann-Whitney, and Kruskal-Wallis test, were conducted to compare the case and control groups. The odds ratio was calculated to see the risk of the assessed variables, including genotype, allele frequency, and the presence of ACE I/D gene polymorphism. Results: In the case group, the frequency of the II genotype was 2 (6.7%), the ID genotype was 25 (83.3%), and the DD genotype was 3 (10.0%). In the control group, the frequency of the II genotype was 2 (6.7%), the ID genotype was 26 (86.7%), and the DD genotype was 2 (6.7%). Statistically, there was no significant association between ACE I/D gene polymorphisms in essential hypertension patients and healthy people (p=0.500; OR=1.556; 95% CI=0.241-10.049). Conclusion: ACE I/D gene polymorphism was not significantly associated with essential hypertension in Cirebon, West Java, Indonesia.    Received: 25 September 2024  |  Reviewed: 21 October 2024  |  Revised: 23 November 2024  |  Accepted: 30 November 2024.
Genetic Polymorphism of Interferon-Gamma +874T/A as a Risk Factor for Pulmonary Tuberculosis in Cirebon, Indonesia Kiteswara, Allain Umadela Deuxawalu; Sari, Ariestya Indah Permata; Pratamawati, Tiar Masykuroh
GHMJ (Global Health Management Journal) Vol. 7 No. 3s (2024)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35898/ghmj-741107

Abstract

Background: Tuberculosis (TB) is one of the leading causes of death globally, caused by Mycobacterium tuberculosis. With 10% of all cases worldwide in 2022, Indonesia is the second-largest contributor of tuberculosis cases. IFN-γ gene polymorphism is one of the factors that have been studied extensively for its association with TB. Aims:  To analyze IFN-γ +874T/A gene polymorphism as a risk factor for pulmonary tuberculosis in Cirebon. Methods: Observational analysis with case control design was used in this study. Thirty-two tuberculosis patients as cases and 32 healthy controls at RSUD Waled were collected and performed DNA extraction to evaluate the polymorphism by using Amplification-refractory mutation system–polymerase chain reaction (ARMS–PCR). Statistical comparison was performed by using Pearson Chi-square and Kruskal Wallis test. Mann-Whitney U test was done for post hoc test. Odds ratio was calculated to see the risk of the assessed variables, including genotype, allele frequency, and the presence of polymorphism. Results:  In the case group, the frequency of TT genotype was 3 (9.4%), TA genotype was 26 (81.3%), AA genotype was 3 (9.4%). In the control group, the frequency of TT genotype was 12 (37.5%), TA genotype was 17 (53.1%), AA genotype was 3 (9.4%). A significant difference (p=0.034) was found among 3 genotype groups. Post hoc test revealed that TT and TA was the pair with significant difference (p=0.007). In addition, TA polymorphism was significantly associated (p=0.004) with tuberculosis (OR=6.614; CI95% = 1.660-26.349). Conclusion: IFN-γ +874 TA gene polymorphism is associated with pulmonary tuberculosis in the population of Cirebon, West Java, Indonesia.   Received: 25 September 2024  |  Reviewed: 23 October 2024  |  Revised: 10 November 2024  |  Accepted: 30 November 2024
Co-Authors Agastya, I Gusti Ngurah Destiwi, Monita Diary, Dwina Gustia Dimas Sondang Irawan Doni Marisi Sinaga, Doni Marisi Donny Nauphar Dyah Anantalia Widyastari Fattah, Rifqi Abdul Hananto, Andang Adi Fitria Jamhari Jamhari Kiteswara, Allain Umadela Deuxawalu Kurniawan, Febrian Hendra Kuswara, Hasna Nurazizah Macnab, Andrew John Made Dian Shanti Kusuma Maretalinia, Maretalinia Moehas, Puput Kusumawardani Ni Wayan Kesari Dharmapatni Noor, Hafizh Muhammad Norsaputra, Ary Pancanugraha, Andi Pratama, Mahendrawan Nugraha Aji Pratamawati, Tiar Masykuroh Pratiwi, Ivana Aprilia Putra, I Gusti Ngurah Edi Putra, Wahyu Manggala Rahman, Pamuko Aditya Samosir, Ricky Alexander Shidiq, Galih Albarra Tiara Fatmarizka Vejvisithsakul, Pichpisith Pierre Viniriani, Marina Ayu Wibowo, Canggih Puspo