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All Journal Syntax Literate: Jurnal Ilmiah Indonesia Indonesian Journal of Medicine Proceedings of the International Conference on Applied Science and Health Global Health Management Journal Tunas Medika Jurnal Kedokteran & Kesehatan
Donny Nauphar
Fakultas Kedokteran Unswagati
Humanities Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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miR-21 Expression in Grade I, II, and III Breast Cancer -, Juwita; Ompusunggu, Henny E.S.; Nauphar, Donny; Pardede, Sumondang M.; Lim, Hadyanto; Siregar, Yahwardiah
Indonesian Journal of Medicine Vol 1, No 1 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Breast cancer is the second largest cancer in the world.The gold standard for breast cancer diagnosis and prognosis is grade assessment (mitotic, nuclear pleiomorphism, tubular formation), based on breast tissue histopathology. However, this assessment has limitation in that it is subjective and semi quantitative. A genetic biomarker known as  microRNA (miRNA) may regulate human gene expression by targetting mRNA. One of the miRNA, i.e. miR-21, is able to suppress tumor suppressor mRNA, resulting in increased cellular proliferation andneoplastic transformation, and decreased apoptosis. This study aimed to investigate the expression of miR-21in breast cancer with histopathologic grade I, II, and III.Subjects and Method: This was a descriptive study with cross-sectional design involving paraffin block (FFPE) samples of ductal-type breast cancer tissue. Sixty four samples were obtained from the department of Anatomical Pathology, Haji Adam Malik Hospital, Medan. Patients history were obtained through medical records followed by sample processing (Total RNA Isolation, cDNA Synthesis, and Examination of miR-21 Expression using Real-Time qPCR). MiR-21 expression from every hystopathologic grade was obtained using Fold Change Calculation.Results: Breast cancer occurred more frequently in age group of 30-49 years old. Based on the histopathologic grade, most breast cancer was found in grade II. Analysis of miR-21 expression showed that miR-21 expression was higher in grade III compared to other grade.Conclusion: Breast cancer occured more frequently in the productive age group.Most breast cancer were found in grade II. Increased miR-21 expression in grade III may suggest that miR-21 is an oncomiR, which plays an important role in the development of malignant breast cancer.Keywords: breast cancer, histopathology grade, miRNA, miR-21.Correspondence: Juwita. Masters Program in Biomedical Sciences, Faculty of Medicine, North SumateraUniversity, Medan. Mobile: 08125262339.Indonesian Journal of Medicine (2016), 1(1): 26-33https://doi.org/10.26911/theijmed.2016.01.01.04
MicroRNA-155 Tissue Expression in Patients with Breast Cancer at Dr Haji Adam Malik Hospital, Medan Ompusunggu, Henny Erina Saurmauli; -, Juwita; Nauphar, Donny; Pardede, Sumondang M.; Lim, Hadyanto; Siregar, Yahwardiah
Indonesian Journal of Medicine Vol 1, No 1 (2016)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: MicroRNA (miRNA) is noncoding-RNA that comprises 18-22 nucleotides. miRNA binds its gene targets in the 3-untranslated region (3-UTR), causing direct degradation of mRNA and repressed translation of RNA. MicroRNA regulates various biological processes, including cell proliferation, apoptosis, cell growth, cell differentiation, and metabolism. Various changes have been identified miRNA expression in different types of cancers. This study aimed to describe the expression of miR-155 in patients with breast cancer tissue in Haji Adam Malik Hospital in Medan.Subjects and Method: This was a descriptive study. A total sample was 64 paraffin tissue blocks was taken from female patients with breast cancer at the surgical oncology unit, Haji Adam Malik Hospital in 2013-2014. RNA sample were isolated and checked using a spectrophotometer followed by cDNA synthesis. Then this sample was analyzed for miR-155 expression by using Real Time qPCR. Histopathological grade data were obtained from the medical record.Results: The miR-155 expression in the breast cancer patients is higher than that in the UniSp6 RNA Spike-in (internal controls). miR-155 expression was 29.64, 28.44, 29.22, in grade-I, grade-II, grade-III breast cancer, respectively. UniSp6 RNA Spike-in was 22.30, 22.89, 23.10, respectively in the control groups. Thus, increased of miR-155 expression was the highest in grade I breast cancer patients.Conclusion: Increased miR-155 expression can be used as a biomarker for early diagnosis and prognosis of breast cancer.Keywords: miRNA, miR-155, breast cancer.Correspondence: Henny Erina Saurmauli Ompusunggu. Department of Cellular and Molecular Biology, Faculty of Medicine, HKBP Nommensen University, Medan. Email: ompusunggu.henny@gmail.comIndonesian Journal of Medicine (2016), 1(1): 20-25https://doi.org/10.26911/theijmed.2016.01.01.03
COMPARISON OF EFFECTIVENESS BETWEEN CELERY JUICE (Apium graveolens L.) AND 2% MICONAZOLE TOWARDS THE GROWTH OF Malassezia furfur Hammada, Ronaa; Pratiwi, Witri; Fauzah, Shofa Nur; Nauphar, Donny; Amanah, Amanah
Proceedings of the International Conference on Applied Science and Health No 3 (2018)
Publisher : Proceedings of the International Conference on Applied Science and Health

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Introduction: Pityriasis versicolor is caused by the fungi Malassezia furfur with a worldwide prevalence of 50%, including tropical countries, second only to dermatitis in Indonesia. Pityriasis versicolor is difficult to treat and requires long-term treatment. The disease has high recurrence risk and may cause drug resistance. 2% Miconazole is known to have long-term side effects; therefore, alternative treatment is needed. Several studies suggested that celery (Apium graveolens L.) contains active substances with anti-fungal properties. This paper aims to investigate the comparison of effectiveness between celery juice and 2% Miconazole towards the growth of Malassezia furfur. Methods: This is an in-vitro experimental study with post-test only control group design. The subjects were split into 5 groups which were given celery juice in 10% DMSO with the concentration of 10%, 20%, 30%, 40%, and 50%. A negative control group was given only 10% DMSO and the positive control group was given 2% Miconazole. The data were then analyzed using Kruskall-Wallis followed by Mann-Whitney test. Results: The Kruskall-Wallis test showed all concentration of celery juice had antifungal effect with p=0.000 (p<0.05) and were effective in inhibiting the growth of Malassezia furfur. The Mann-Whitney test showed that the 50% celery concentration was as effective as 2% Miconazole in inhibiting the growth of Malassezia furfur (p=0.495). Conclusion: Celery juice (Apium graveolens L.) was effective in inhibiting the growth of Malassezia furfur with 50% concentration as the most effective concentration. 
ANTENATAL CARE (ANC) VISIT FREQUENCY IN THE THIRD TRIMESTER AND PRETERM EVENTS IN PUSKESMAS PONDOH AND JUNTINYUAT IN INDRAMAYU REGENCY BETWEEN 2014-2016 Ameliana, Elinda; Nauphar, Donny; Maryam, Ruri Eka
Proceedings of the International Conference on Applied Science and Health No 3 (2018)
Publisher : Proceedings of the International Conference on Applied Science and Health

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Background: World Health Organization (WHO) estimates that 15 million babies are born preterm every year and this number continues to increase. Several risk factors for preterm are identified as mother’s lifestyle such as smoking, malnutrition, weight gain during pregnancy, and drug use or other factors such as socioeconomic status. In 2015, WHO reported more than 300.000 women died from pregnancy-related causes and 2.6 million babies were still born worldwide with half occurring during the third trimester. Antenatal Care (ANC) in the third semester aims to identify fetal position and identify complication during pregnancy and screen for pre-eclampsia, infection of the reproductive organs and urinary tract, and plan for delivery. The ANC in the third semester is crucial in keeping pregnant mother healthy throught pregnancy and delivery. Aim: To find out the relationship between third trimester Antenatal Care (ANC) visit frequency and preterm birth event at Puskesmas Pondoh and Juntinyuat in Indramayu regency between 2014-2016. Methods: This was observational analytic study with cross-sectional approach. The sampling technique in this research was done by total sampling with 100 respondens. Data were collected from maternal cohort book and control card of pregnant women. The data were analyzed using Chi-square test and Prevalence Ratio. Results: This study found that the proportion of pregnant mothers with insufficient third trimester ANC was 38% and the number of mothers delivering preterm baby was 32%. Statistical analysis showed that there was a statistical significance between third trimester ANC visit frequency and preterm labor with p=0,000 (p<0,05) and women with insufficient ANC visit have 40 times higher risk of preterm baby compared to mothers who has sufficient ANC visit (PR=40,60; CI95%=11.699-140.8862) in Puskesmas Pondoh and Juntinyuat Indramayu District 2014-2016. Conclusion: Having less than 4 ANC visit in the third trimester may increase the risk of delivering preterm babies. Pregnant women are advised to do ante natal care monthly of a minimum 4 times during the third trimester. 
CHANGING COSMETIC BRANDS INCREASE RISK OF FREQUENCY AND DEGREE OF ACNE VULGARIS IN FEMALE UNDERGRADUATE STUDENTS Maharani, Annida; Pratiwi, Witri; Nauphar, Donny
Proceedings of the International Conference on Applied Science and Health No 3 (2018)
Publisher : Proceedings of the International Conference on Applied Science and Health

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Background: Acne vulgaris is a skin disease caused by chronic inflammation of the follicular pilosebacea marked by the presence of blackheads, papules, pustules, nodules, and cysts in place of healthy skin. One of the risk factors for Acne vulgaris is changing cosmetic brands which may contain comedogenic and acnegenic ingredients. The aim of this study is to find out the relationship of changing cosmetic brands and the prevalence of Acne vulgaris in female undergraduates. Methods: This was an observational study with cross sectional design. The subjects were 344 female undergraduates who used two out of four types of cosmetics, recruited by total sampling. Data were analyzed to see the relationship of changing cosmetic brands with the degree and the frequency of acne. Results: Based on Spearman correlation test, changing cosmetic brands has a strong positive correlation (r2= 0.762) with the degree of acne and a mild positive correlation (r2=0.461) with acne frequency. Chi-square test showed statistical significance (p<0.05) between changing cosmetic brands and the degree and frequency of acne. Subjects who frequently changes cosmetics has a 66 times higher risk of having bad acne (PR= 66.994; CI 95% = 32.099–139.320) and 8 times higher risk of having more frequent acne (PR= 8.432; CI 95% = 4.969-14.308) compared to subjects that do not frequently change cosmetics. Conclusion: Changing cosmetic brands increased the risk for both the degree of acne and the frequency of acne. 
THE EFFECT OF ORALLY ADMINISTERED CATFISH (Clarias gariepinus) SKIN AND MEAT ON EPITHELIALIZATION THICKNESS AND COLLAGEN DENSITY IN INCISION WOUND OF WISTAR RAT (Rattus norvegicus) Baihaqi, Muhammad Irsyad; Satrianugraha, Muhammad Duddy; Pratamawati, Tiar M; Nauphar, Donny
Proceedings of the International Conference on Applied Science and Health No 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Background: Alternative medicine using animal is still rare whereas Indonesia have a lot of potential natural resources. Catfish is easily found animal in Indonesia and is common consumed as nutriment sources contains albumin, amino acid and fatty acids which plays an essential role of wound healing process. This study will determine effect of orally administered catfish (Clarias gariepinus) skin and meat on epithelialization thickness and collagen density in incision wound of white male rat (Rattus norvegicus).Methods: This experimental post-test only group design used 30 white male rat (Rattus norvegicus), that randomly divided into 4 tratment goup and one control group. The treatment group was given 12,5 mg/g W, 25 mg/g W, 37,5 mg/g W and 50 mg/g W dose of catfish (Clarias gariepinus) skin and meat flour, in the other hand the contol group were given aquades. Non-parametric analyses using Kruscall-Wallis and Mann Whitney test were used to compare epithelization thickness and collagen density.Result: The comparison results of epithelialization thickness between group C with TGI, C with TG2, C with TG3 and C with TG4 showed significant differences (p<0.05). TG4 was the thickest of all groups. Significant differences were also observed in collagen density result between C with TGI, C with TG2, C with TG3 and C with TG4 (p <0,05) TG4 showed the highest density of all groups.Conclusion: Catfish (Clarias gariepinus) skin and meat flour with 50 mg/200g W dose is effective for wound healing and increasing epithelial thickness and collagen density in Wistar rat (Rattus norvegicus) incision wounds.
EFFECT OF PHYSICAL ACTIVITY AND FAST FOOD CONSUMPTION FREQUENCY ON OVERWEIGHT LEVEL AMONG HIGH SCHOOL ADOLESCENT Harlianti, Lita; Pratiwi, Witri; Nauphar, Donny; Suhaeni, Eni
Proceedings of the International Conference on Applied Science and Health No 3 (2018)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Background: Obesity was believed to be one of the risk factors of degenerative diseases, such as diabetes mellitus, hypertension, and coronary heart disease. Obesity occurred gradually that started with overweight. Risk factors for overweight are lack of physical activity, and consumption of high caloric fatty food which may cause accumulation of fats inside the body. This research aims to examine the effect of physical activity and fast food consumption frequency on overweight level among high school adolescent in Cirebon. Methods: It was a cross-sectional study conducted among 262 high school students aged 15-17 years using stratified random sampling. Results: The result based on Spearman correlation test showed negative correlation between physical activity and overweight level with p= 0,001 (p< 0,05) and correlation coefficient of -0,627. There was positive correlation between consumption fast food frequency with overweight with p=0,037 (p=0,05) and correlation coefficient of 0,423. Conclusions: Physical activity and fast food consumption frequency have effects on overweight level in high school adolescent in Cirebon. 
EFFECTIVENESS OF ORALLY USE CATFISH (Clarias gariepinus) SKIN AND MEAT ON WOUND LENGTH AND FIBROBLAST DENSITY IN INCISION WOUND OF WISTAR RAT (Rattus norvegicus) Fauziah, Sri Utami; Primanagara, Risnandya; Pratamawati, Tiar M; Nauphar, Donny
Proceedings of the International Conference on Applied Science and Health No 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Background: Treatment for wounds that are currently often given is use povidone-iodine. But, this antiseptics also kills fibroblast tissue which is useful for forming new tissue. One of the other ways to treatment for wounds is use catfish. The contents found in catfish (Clarias gariepinus) is albumin which is a globular protein that is useful in the formation of body tissues, such as postoperative wounds and burns. This study aims to determine the effect of skin and meat of catfish (Clarias gariepinus) on wound length and fibroblast density in incision wounds of Wistar rat (Rattus norvegicus).Methodology: This study is an experimental study with Post Test Only Control Group Design using Wistar rats which are divided into five groups. The control group (aquadest) and treatment groups 1, 2, 3 and 4 are given the skin and meat of catfish (Clarias gariepinus) orally at a dose of 12.5 mg / 200 g BW, 25 mg / 200 g BW, 37.5 mg / 200 g BW, and 50 mg / 200 g BW. After 10 days, the rats are killed to take the wound tissue for histological preparations to observe fibroblast density. Then, it is analyzed by ANOVA test or the alternative.Results: There is an effect of giving catfish skin and meat (Clarias gariepinus) orally at different dose. The administration of catfish skin and meat (Clarias gariepinus) at a dose of 37.5 mg / 200 g BW and 50 mg / 200 g BW is better than other doses assessed from wound length and fibroblast density in Wistar rat incision wounds (Rattus norvegicus).Conclusion: The use of catfish for wound healing can be applied. This method can reduce wound length and increase fibroblast density at certain doses.
EFFECTIVENESS OF ORALLY USE TILAPIA (Oreochromis niloticus) ON WOUND LENGTH AND FIBROBLAST DENSITY ON INCISION WOUND OF WISTAR RAT (Rattus norvegicus) Syauqia, Sayyidah Vina; Zein, A Fariz Malvi Zamzam; Pratamawati, Tiar M; Nauphar, Donny
Proceedings of the International Conference on Applied Science and Health No 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Background: The incidence of a wound in Indonesia has increased from 7.5 percent in 2007 to 8.2 percent in 2013 according to Riskesdas. Wound care that widely used is povidone-iodine, but in case, it has a toxic effect on healthy cells around the wound. The albumin, amino acids, and fatty acids in Tilapia  (Oreochromis niloticus) are the potential substances to accelerate the wound healing process. This study aims to investigate the efficacy of Tilapia (Oreochromis niloticus) on wound length and fibroblast density on the incision wound of Wistar rat (Rattus norvegicus).Methods: The study was conducted in the Food and Nutrition PAU Laboratory and Pathology Anatomy Laboratory of Universitas Gadjah Mada, Yogyakarta. This post-test only control group design experimental study used 30 male white rats that randomly divided into five groups, i.e. control group K, and treatment group P1, P2, P3 and P4 that were each given Tilapia with a dose of 12.5 mg/200gBW, 25 mg/200gBW, 37.5 mg/200gBW dan 50 mg/200gBW. The length of the wound was measured by a ruler each day. The rats were terminated on the 10th day to obtain wound tissue for H&E stained histopathological sections to observe fibroblast density. One Way ANOVA, Tamhane, Kruskal-Wallis and Mann Whitney tests were used to compare the wound length and fibroblasts density.Results: Incision wounds in this study showed the best wound healing on the 10th day. Based on the theory, on the 10th day, a proliferation and remodeling phase occurs. There is an effect of giving Tilapia (Oreochromis niloticus) orally at a different dose. Tilapia at dose 37.5mg/200gBW is more effective compared with the dose of 12.5mg/200gBW, 25mg/200gBW, and 50mg/200gBW to accelerate wound healing for wound length and fibroblast density in Wistar rat (Rattus norvegicus).Conclusion: Consuming Tilapia (Oreochromis niloticus) could be a new alternative for wound healing treatment. The antimicrobial ability of Tilapia should be analyzed.
The International Conferences on Applied Science and Health (ICASH): Efforts and Commitments in Translating Research Results into Policy and Practices, for a Better Society Widyastari, Dyah Anantalia; Sinaga, Doni Marisi; Wibowo, Canggih Puspo; Rahman, Pamuko Aditya; Noor, Hafizh Muhammad; Irawan, Dimas Sondang; Fatmarizka, Tiara; Diary, Dwina Gustia; Putra, I Gusti Ngurah Edi; Maretalinia, Maretalinia; Viniriani, Marina Ayu; Destiwi, Monita; Shidiq, Galih Albarra; Pratiwi, Ivana Aprilia; Kusuma, Made Dian Shanti; Dharmapatni, Ni Wayan Kesari; Pratama, Mahendrawan Nugraha Aji; Norsaputra, Ary; Pancanugraha, Andi; Kurniawan, Febrian Hendra; Putra, Wahyu Manggala; Fattah, Rifqi Abdul; Moehas, Puput Kusumawardani; Samosir, Ricky Alexander; Hananto, Andang Adi Fitria; Agastya, I Gusti Ngurah; Susilo, Taufik Eko; Vejvisithsakul, Pichpisith Pierre; Nauphar, Donny; Sari, Ariestya Indah Permata; Macnab, Andrew John
GHMJ (Global Health Management Journal) Vol 3, No 3 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Abstract

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Co-Authors A Fariz Malvi Zamzam Zein Agastya, I Gusti Ngurah Akrom, Akrom amanah amanah Amanah Amanah Ameliana, Elinda Anak Agung Istri Sri Wiadnyani Andang Adi Fitria Hananto Andi Pancanugraha Andrew John Macnab Annida Maharani Ariestya Indah Permata Sari Ary Norsaputra Baihaqi, Muhammad Irsyad Canggih Puspo Wibowo Destiwi, Monita Diary, Dwina Gustia Dimas Sondang Dimas Sondang Irawan Doni Marisi Sinaga Doni Marisi Sinaga, Doni Marisi Dwina Gustia Diary Dyah Anantalia Widyastari Dyah Anantalia Widyastari Elinda Ameliana Eni Suhaeni Fattah, Rifqi Abdul Fauzah, Shofa Nur Fauziah, Sri Utami Febrian Hendra Kurniawan Galih Albarra Shidiq Hadyanto Lim Hafizh Muhammad Noor Hammada, Ronaa Hananto, Andang Adi Fitria Harlianti, Lita Hendry Gunawan, Hendry Henny Erina Saurmauli Ompusunggu Husna, Nazaul I Gusti Ngurah Agastya I Gusti Ngurah Edi Putra Ivana Aprilia Pratiwi Jamhari Jamhari Juwita -, Juwita Kurniawan, Febrian Hendra Kuswara, Hasna Nurazizah Lita Harlianti Macnab, Andrew John Made Dian Shanti Kusuma Maharani, Annida Mahendrawan Nugraha Aji Pratama Maretalinia Maretalinia Maretalinia Maretalinia, Maretalinia Marina Ayu Viniriani Maryam, Ruri Eka Moehas, Puput Kusumawardani Monita Destiwi Muhammad Irsyad Baihaqi Ni Wayan Kesari Dharmapatni Noor, Hafizh Muhammad Norsaputra, Ary Oktaviyati, Nurfithria Ompusunggu, Henny E.S. Pamuko Aditya Rahman Pancanugraha, Andi Pardede, Sumondang M. Pichpisith Pierre Vejvisithsakul Pradana, Muhammad Firyal Pratama, Mahendrawan Nugraha Aji Pratamawati, Tiar M Pratamawati, Tiar Masykuroh Pratiwi, Ivana Aprilia Puput Kusumawardani Moehas Putra, I Gusti Ngurah Edi Putra, Wahyu Manggala Putri, Annisa Septiani Putri Rahman, Pamuko Aditya Ricky Alexander Samosir Rifqi Abdul Fattah Risnandya Primanagara Ronaa Hammada Ruri Eka Maryam Samosir, Ricky Alexander Sari, Ariestya Indah Permata Satrianugraha, Muhammad Duddy Sayyidah Vina Syauqia Shidiq, Galih Albarra Shofa Nur Fauzah Sri Utami Fauziah Syauqia, Sayyidah Vina Tiar M Pratamawati Tiara Fatmarizka Vejvisithsakul, Pichpisith Pierre Viniriani, Marina Ayu Wahyu Manggala Putra Wibowo, Canggih Puspo Witri Pratiwi Witri Pratiwi Yahwardiah Siregar Yuny Hafitry Zein, A Fariz Malvi Zamzam