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All Journal Indian Journal of Forensic Medicine & Toxicology
David S. Perdanakusuma
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Public Health

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Difference in DNA Methylation between Cleft Lip and Cleft Lip and Palate Ruby Riana Asparini; David S. Perdanakusuma; Retno Handajani; Henydhar Bramastivira Mahdani; Sulistyo Mulyo Agustini
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17630

Abstract

It is suspected that environmental exposure to non-syndromic oral clefts, which includes cleft lip (CL), cleft lip and palate (CLP) has an effect on epigenetic mechanisms, particularly deoxyribonucleic acid (DNA) methylation. DNA methylation will be expressed during facial morphogenesis and have an impact on facial development. This study aimed to observe differences in DNA methylation between CL and CLP, between CL mothers and CLP mothers, and correlation between CL and CL mothers, CLP and CLP mothers.This observational study used a sample of 13 patients diagnosed with CL and 14 patients diagnosed with CLP and their respective mothers. The test was performed using ELISA MethylFlashTM Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric).The median DNA methylation at CLP was 1.92 (0.23 - 14.07) and CL was 1.71 (0.08 - 8.47) (p 0.752 > 0.05)). Median DNA methylation in CLP mothers was 0.997 (0.03 - 6.14) and in CL mothers 0.72 (0.23 - 6.16) (p 0.798 (p> 0.05). Correlation test for DNA methylation of CLP with CLP mother r = -0.259 and (p = 0.394 >0.05). Correlation test for DNA methylation of CL patients with CL mothers revealed r = -0.492 and (p = 0.087 > 0.05).The results of this study showed no difference in methylation between CL and CLP. This study found that DNA methylation between CL mothers and CLP mothers was relatively the same. This study also found no correlation between DNA methylation of CL and CL mothers, and between CLP and CLP mothers.
Distribution of Cleft Lip and Palate at Cleft Lip and Palate Center Medical Faculty, Universitas Muhammadiyah Malang, Indonesia Ruby Riana A; David S. Perdanakusuma; Retno Handayani
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15927

Abstract

Cleft lip and palate (CLP) and cleft palate (CP)are a common head and neck hereditary deformity. The highnumber of cases has prompted the Medical Faculty, Universitas Muhammadiyah Malang to start communityservice by providing free surgery to repair cleft lip and palate (Cleft Lip and Palate Center Medical Facultyof Universitas Muhammadiyah Malang (CLP MFUMM)).Purpose: To find the distribution of cases from the year of surgery, sex, side of CL/P, cleft palate (CP),hospital where the surgery was performed and parent education level in CLP MFUMM, from 2019 until2020.Patients and Methods: Two hundred and five consecutive cleft lip subjects, with or without palates, andcleft palate attending CLP MFUMM from 2019 until 2020 were studied.Results:The most surgeries done is at the year of 2019 (69%). Most of the patients were male (55%).CL/P were the most frequent cases (94%). Complete cleft found mostly in male patient with left unilateralcleft (23%), while incomplete cleft found mostly in female patient also with left unilateral cleft (7%). Thepalate involvement found mostly in soft palate only and female patients (46%). The surgeries were mostlyperformed at RSI Aisyiyah Malang (60%). The education level of the parents is mostly elementary school(father 36%, mother 34%).Conclusion: CL/P were the most frequent. Complete cleft mostly in male patient with left unilateral cleft,while incomplete cleft mostly in female patient also with left unilateral cleft. The cleft palate was mostlysoft palate only and female patients.
Co-Authors Henydhar Bramastivira Mahdani Retno Handajani Retno Handayani Ruby Riana A Ruby Riana Asparini Sulistyo Mulyo Agustini