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All Journal Jurnal Veteriner Microbiology Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Biomedical Engineering Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease WMJ (Warmadewa Medical Journal) YARSI Medical Journal Biomolecular and Health Science Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Indian Journal of Forensic Medicine & Toxicology Jurnal Keperawatan Soedirman (The Soedirman Journal of Nursing)
Retno Handajani
Department Of Biochemistry, 00Faculty Of Medicine, Universitas Airlangga, Surabaya
Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

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Karsinoma Nasopharynx dan infeksi EBV di Indonesia; analisis aspek klinis, Patologi dan Biomolekular Notopuro, Harianto; A Kentjono, Widodo; Handajani, Retno; B Notopuro, Paulus
Jurnal Kedokteran YARSI Vol 16, No 2 (2008): MEI - AGUSTUS 2008
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (376.428 KB) | DOI: 10.33476/jky.v16i2.239

Abstract

Nasopharyngeal carcinoma (NPC) is a unique epithelial malignancy that occurs at a high frequency in certain regions of Southeast Asia. Previous study revealed the association between Epstein Barr Virus (EBV) and to a lesser extent, Human Papilloma Virus (HPV) with NPC. The role of the EBV in the pathogenesis of NPC was further supported by the recovery response of the tumor cells after being treated with EBV-specific T lymphocytes. The present study aims to determine the frequency distribution of EBV infection among the pathologically confirmed NPC patients and healthy control in Surabaya, Indonesia. The presence of Human Papilloma. Virus infection (HPV) was also analysed. The results indicated that EBV DNA existed in the 88% of the nasopharyngeal tissue biopsies of 25 NPC patients and none in the 10 healthy controls, and the difference was statistically significant.Analysis of the mononuclear cells of peripheral blood revealed that 60% of the 25 NPC patients carried EBV DNA whereas in control group 20% was found to be positive and the difference was statistically significant. Although a high positive rate EBV-DNA was detected in NPC patients, additional environmental and genetic factors must still be considered. Nevertheless, no HPV-DNA was detected from mononuclear cells of peripheral blood and nasopharyngeal tissue of the two groups. By this study there was no coexistence between the infection of EBV and HPV which promote carcinogenesis in NPC. Analysis using LMP1-DNA in tumor cell biopsies indicated that 72% of the NPC patients yielded PCR products and none of the healthy control, the difference was statistically significant. In conclusion the findings confirm the tight association between the EBV and NPC in Indonesia and that the specific presence of LMP1-DNAs in the tumor cells strongly indicates the important role of EBV in the pathogenesis of NPC. Coexistence of EBV and HPV infections was not found in NPC cases in Indonesia.
Socialization Program for Prevention and Early Detection of Congenital Hearing Loss in the Families of Deaf School children Gwenny Ichsan Prabowo; Citrawati Dyah Kencono Wungu; Retno Handajani; Nyilo Purnami; Fis Citra Ariyanto
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16878

Abstract

Objective: socializing hearing loss examination and early detection to patients and their families in deaftype B schools. Methods: A community service program in the form of socialization was performed to thefamily of patients with hearing loss in deaf school type B, Surabaya, Indonesia. Pretest and posttest wereconducted to determine the initial understanding and post socialization knowledge of these people. We alsoasked the participants to fill on a questionnaire regarding the possible causes of the hearing loss (familyhistory, drug use, history of disease, and history of head trauma). Results: Based on the summary of pretestand posttest from the participants, an increase in participants’ knowledge of hearing lost was found. Thisactivity was attended by 90 family members of 37 patients with hearing loss. Pre and post test results. Theresults of the questionnaire showed that 94.59% of the patients came from Javanese ethnicity. As many as21.62% of patients had a family history of hearing loss, and even 2 patients had a father, mother, and siblingwith hearing loss. A total of 18.92% had a history of using ototoxic drugs, 16.22% had a history of maternalRubella infection during pregnancy, and 2.7% had a history of head trauma. Conclusion: Socializationprogram was effective to increase knowledge of congenital hearing loss for family of deaf schoolchildren.The result of the questionnaire showed that deaf schoolchildren had several risk factors for the occurrenceof hearing loss. Similar program can be performed in communities in other areas to increase prevention andearly detection of hearing loss in Indonesia.
Phylogenetic Analysis Related to Hepatitis B Virus based on Region Surfaces in Tuban, East Java Province, Indonesia Supiana Dian Nurtjahyani; Retno Handajani
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.17103

Abstract

Hepatitis B virus (HBV) is a virus cause liver disease. This virus could be transmitted through blood product,and it becomes the major problems in developing countries. Genotype distribution of HBV depends ongeographical characteristics. Here, we reported HBV genotyping analysis of blood donors with positiveHepatitis Surface Antigen (HbsAg) at Indonesian Red Cross Foundation in Tuban, East Java. Total 150sera were collected from blood donors at Indonesian Red Cross Foundation in Tuban, East Java. Enzyme-Linked Immunosorbent Assay (ELISA) was conducted by PRISM®HBsAg kit (ABBOTT) according tothe manufacture’s instruction, to determine sample that has positive HbsAg. The genotyping analysis wasdone using polymerase chain reaction and sequencing method from 12 samples. Genetyx ver.10 softwarewas used for identification of HBV type. Analysis of HBV genotype revealed three different types of HBV.HBV type B was the major HBV (50%). Interestingly, there was one unidentified HBV type suggested as anovel subtype of HBV. Further genotyping analysis is needed to confirm novelty of the unidentified HBVtype. Analysis of HBV genotype revealed three different types of HBV. HBV type B was the major HBV(50%). Interestingly, there was one unidentified HBV type suggested as a novel subtype of HBV. Furthergenotyping analysis is needed to confirm novelty of the unidentified HBV type. This study was the firstevidence of blood donor’s genotyping analysis in Tuban.
Reduction Surgery of Giant Hemifacial Neurofibromas: A Case Report Ruby Riana A; Sitti Rizaliyana; Radias Dwi Padmani; Retno Handajani; S. Perdanakusuma
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17628

Abstract

Neurofibromatosis type 1 (NF-1), first described by Von Recklinghausen (1882), is an autosomal dominant disease caused by a spectrum of mutations in NF-1 gene. We reported a case of giant facial neurofibromas in 26 years old male patient without family history of neurofibromatosis type 1. Features typical of neurofibroma, including an enlarged nerve fascicle composed of elongated nuclei and scant cytoplasmic cells, were identified. Excision was performed with preservation of parotid duct. The mass weight was about 5,5 kg. There were some challenges during the procedure, such as bleeding, hypovolemia, and preservation of vital structure.
Difference in DNA Methylation between Cleft Lip and Cleft Lip and Palate Ruby Riana Asparini; David S. Perdanakusuma; Retno Handajani; Henydhar Bramastivira Mahdani; Sulistyo Mulyo Agustini
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17630

Abstract

It is suspected that environmental exposure to non-syndromic oral clefts, which includes cleft lip (CL), cleft lip and palate (CLP) has an effect on epigenetic mechanisms, particularly deoxyribonucleic acid (DNA) methylation. DNA methylation will be expressed during facial morphogenesis and have an impact on facial development. This study aimed to observe differences in DNA methylation between CL and CLP, between CL mothers and CLP mothers, and correlation between CL and CL mothers, CLP and CLP mothers.This observational study used a sample of 13 patients diagnosed with CL and 14 patients diagnosed with CLP and their respective mothers. The test was performed using ELISA MethylFlashTM Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric).The median DNA methylation at CLP was 1.92 (0.23 - 14.07) and CL was 1.71 (0.08 - 8.47) (p 0.752 > 0.05)). Median DNA methylation in CLP mothers was 0.997 (0.03 - 6.14) and in CL mothers 0.72 (0.23 - 6.16) (p 0.798 (p> 0.05). Correlation test for DNA methylation of CLP with CLP mother r = -0.259 and (p = 0.394 >0.05). Correlation test for DNA methylation of CL patients with CL mothers revealed r = -0.492 and (p = 0.087 > 0.05).The results of this study showed no difference in methylation between CL and CLP. This study found that DNA methylation between CL mothers and CLP mothers was relatively the same. This study also found no correlation between DNA methylation of CL and CL mothers, and between CLP and CLP mothers.
Time-dependent Expression of Caspase-3 and Degeneration of Lateral Rectus Muscle on Experimental Esotropia in Rabbits Luki Indriaswati; Nurwasis; Gatut Suhendro; Soetjipto; Retno Handajani
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14785

Abstract

Background: To identify correlation between caspase-3 expression and degeneration of lateral rectus muscleon experimental esotropia in rabbits by using time dependent analysis. Methods: Twenty four rabbits weredivided into 4 groups, first month sham (S1); second month sham (S2); first month esotropia (E1); andsecond month esotropia (E2) model groups. Immunohistochemical and haematoxyllin eosin staining wereperformed. Results: In first month observation no significant difference in caspase-3 expression between S1(1.56 ± 1.03) and E1 (3.93 ± 1.66) groups (p >0.05) and in the degeneration score of lateral rectus musclebetween S1 (1.33 ± 1.36) and E1 (3.83 ± 2.04) groups (p> 0.05). In second month observation no significantdifference in caspase-3 expression between S2 (1.80 ±1.08) and E2 (6.50 ± 3.26) groups (p >0.05); butthere was significant difference in the degeneration score of lateral rectus muscle between S2 (1.16 ± 1.16)and E2 (4.50 ± 1.04) groups (p <0.05). There was a significant correlation between caspase-3 expressionand the degeneration score of lateral rectus muscle (p=0.020; r=0.471). Conclusion: On the experimentalesotropia with prolonged lateral muscle contraction in rabbits, the increasing trend of caspase-3 expressionhas a correlation with the increasing degeneration score of lateral rectus muscle.
Detection of Hepatitis C Virus RNA in Blood Donors with Nested PCR Technique Supiana Dian Nurtjahyani; Mochammad Amin Amin; Retno Handajani Handajani
Biomedical Engineering Vol 2, No 2 (2016): Biomedical Engineering
Publisher : Biomedical Engineering

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (448.443 KB)

Abstract

Objective: This study was conducted to detect hepatitis C virus (HCV) RNA among blood donors in Tuban, East Java, Indonesia,  using nested Polymerase Chain Reaction (PCR) technique.Material and methods: Seven blood donors  with positives anti-HCV were obtained from 500 blood donors from Red Cross Blood Donor Center (Palang Merah Indonesia = PMI) Tuban, East Java, Indonesia, consisting of 375 men (75%) and 125 women (25%). Detection of anti-HCV antibody prevalence in the blood donors was done by using Enzyme-Linked Immunosorbent Assay (ELISA).  The detection of HCV RNA in blood donors  sera  with positives  anti-HCV were performed using nested-PCR technique with three set of primer pairs targeting the NS5B and 5’UTR regions of HCV.  Further amplification products were visualized on a 2% agarose gel containing ethidium bromide under  UV illumination.Result: Seven positives anti-HCV blood donors sera (1.4%) were obtained from 500 blood donors sera, in PMI Tuban, East Java, Indonesia. Out of seven blood donors sera with positives anti-HCV, 6 sera (85,7%)  were positives HCV RNA by using nested PCR technique. Five HCV RNAs were detected with two set of primers based on NS5B HCV region and 1 positive HCV RNA was detected with another set of primer based on 5’UTR HCV region.Conclusion: HCV RNAs were detected in 85,7% positives anti-HCV blood donors using nested PCR technique, in PMI Tuban, East Java. Further research is needed to determine the  genotype  and  subtype  of  HCV. 
Detection of Gen Chloramphenicol Acetyl Transferase (CAT ) Salmonella typhi Resistant Chloramphenicol by Polymerase Chain Reaction (PCR) Technique Supiana Dian Nurtjahyani; Retno Handajani
Biomedical Engineering Vol 3, No 1 (2017): Vol. 3 No. 1 Biomedical Engineering
Publisher : Biomedical Engineering

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.416 KB)

Abstract

Objective: Salmonella typhi as a causative agent of typhoid abdominalis many militias, which was resistant to the drug, one of which is chloramphenicol. Detection of Salmonella typhi serologically generally done so if there is a problem in the diagnosis difficult to overcome so it needs a faster diagnosis techniques with accurate results. PCR can be used as an alternative solution to these problems. The purpose of this study was to detect the DNA of Salmonella typhi resistant to chloramphenicol by PCR.Material and methods: The method in this population experimental laboratory conducted in the laboratory biology and laboratory Unirow Tuban ITD Airlangga University Surabaya. Experiment done using PCR from blood samples. Using primer from CAT gene to detect this gene.Result: The results of this study with tank PCR can detect DNA of chloramphenicol resistant Salmonella typhi with a length of 293 bp.Conclusion: The conclusion PCR technique can detect DNA of Salmonella typhi resistant to chloramphenicol.
Distribution of Hepatitis B Virus Genotypes Among Patients at Internal Medicine Unit, Dr. Soetomo General Hospital, Surabaya Citrawati Dyah Kencono Wungu; Mochamad Amin; Ulfa Kholili; Gwenny Ichsan Prabowo; Poernomo Boedi Setiawan; Soetjipto Soetjipto; Retno Handajani
WMJ (Warmadewa Medical Journal) Vol 4 No 1 (2019): May 2019
Publisher : Warmadewa University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22225/wmj.4.1.1032.6-13

Abstract

Background: Hepatitis B virus (HBV) infection is a major health problem worldwide, especially in developing countries. The study of HBV genotypes is important to find out the diversity of HBV genotypes related to the severity of the disease, response to therapy, and clinical symptoms. Objective: This study was aimed to detect HBV genotypes in patients at Hepatology Outpatient Clinic, Dr. Soetomo Hospital Surabaya. Methods: This study was conducted on new patients at Hepatology Outpatient Clinic of Dr. Soetomo General Hospital, Surabaya in one month. Nested PCR was performed by targetting HBV surface genes. Samples with positive HBV DNA were sequenced and analysed further. Results: In this study, a total of 27 samples were obtained. The prevalence of HBV infection shown by positive HBsAg in patients with symptoms of liver disease was 55.55% (15/27 patients). Based on the results of electrophoresis from PCR products, positive HBV DNA was obtained in these 15 patients (100%). After sequencing samples with positive HBV DNA, genotype B of Indonesian strain was found to be predominant genotype (100%). Subgenotype analysis showed that 7/15 samples had B3 subgenotype (46.67%). Conclusion: In patients at Hepatology Outpatient Clinic of Dr. Soetomo General Hospital, Surabaya, the prevalence of HBV infection was high (55.55%) and genotype B was predominant. In Surabaya, HBV genotype infection still remained like the previous pattern, although in Indonesia there have been many inter-island and ethnic migration. Further similar studies are needed to obtain the diversity of other HBV genotypes.
Ekstrak Buah Delima Terstandar Menurunkan Derajat Fibrosis Hati pada Hewan Model Tikus Putih (STANDARDIZED POMEGRANATE FRUIT EXTRACT REDUCES LIVER FIBROSIS DEGREE ON ALBINO RATS (RATTUS NORVEGICUS) AS AN ANIMAL MODEL) Wiwik Misaco Yuniarti; Retno Handajani; Hernomo Ontoseno Kusumobroto; Ketut Sudiana
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.185 KB)

Abstract

The objective of this study was to analyse the effect of standadized pomegranate fruit extracton liver fibrosis due to biliary obstruction.  Liver fibrosis was induced with bile duct ligation (BDL)technique.  Examinations were performed on the expressions of collagen type 1 and the degree ofliver fibrosis. Thirty two male albino rats (Rattus norvegicus), 2.5 month old, weighing 160-190grams were divided into four experimental groups.  The first group (P0) consisted of rats  whichunderwent laparotomy and treated with 2 ml of  carboxymethyl cellulose (CMC) 0.3%.  Three othergroups consisted of rats which underwent laparotomy and bile duct ligation (BDL) but receiveddifferent treatments.  Group P1 was given carboxymethyl cellulose (CMC) 0.3%, P2 was treatedwith standardized pomegranate fruit extract 150 mg/kgBW/po/day within CMC 0.3% , and P3 wasgiven ellagic acid (EA) 60 mg/kgBW/po/day of equal volume.  Treatments were administered on thesecond day after BDL for 21 days.  The livers were excised one day after the last administration.Observations were made on the expressions of collagen type 1 and the degree of liver fibrosis. Theresults showed that standardized pomegranate extract can inhibit the expression of collagen type Isignificantly in the P2 and P3 compared with P1 group (p <0.05). Treatment with standardizedpomegranate fruit extract also significantly suppressed the progression of liver fibrosis comparedto P1, eventhough there was more liver fibrosis compared to P0 (p<0,05).  The degree of liverfibrosis was not significantly different between P2 and P3, and between P3 and P1 (p>0,05).The administration of standardized pomegranate fruit extract 150 mg/kgBW/po/day  exertedantifibrotic effect by inhibiting the increase the expression of collagen type I and the degree of liverfibrosis.
Co-Authors A Kentjono, Widodo Afrina Zulaikah Amin, Indah Binti Mohd Ayesie Natasha Zulka B Notopuro, Paulus Bakhtiar, Arief Bayu Satria Wiratama David S. Perdanakusuma Edhi Rianto Elvaretta, Clarissa Ely Rahmatika Nugrahani Fis Citra Ariyanto Gatut Suhendro Ginanjar Sasmito Adi HAK HOTTA Harianto Notopuro Hendri Susilo Hendy Muagiri Margono Henydhar Bramastivira Mahdani Hernomo Ontoseno Kusumobroto Humairah, Ira I Gusti Agung Komang Diafari Djuni Hartawan Indri Safitri Indri Safitri, Indri Juniastuti Juniastuti Kawilarang, Arthur Pohan Khaerunnisa, Siti Luki Indriaswati Lukitasari, Lina Margarita Maramis, Margarita MARIA INGE LUSIDA Maria Inge Lusida MOCHAMAD AMIN Mochamad Amin MOCHAMAD AMIN Mochamad Yusuf Alsagaff Mochammad Amin Mochammad Thaha MOCHAMMAD THAHA Nurwasis Nurwasis Nyilo Purnami Palupi, Dian Eka Roro Poernomo Boedi Setiawan Prabowo, Gwenny Ichsan Pranawa Pranawa Priyo Budi Purwono Qurnianingsih, Ema Radias Dwi Padmani Rizaliyana, Sitti Ruby Riana A Ruby Riana Asparini Ruslan, S. Eriaty N. S. Perdanakusuma Samira Assegaf Siti Khaerunnisa SOETJIPTO SOETJIPTO . SOETJIPTO . Soetjipto Soetjipto Sulistyo Mulyo Agustini Supiana Dian Nurtjahyani Susi Wahyuning Asih Suwandito Suwandito Syafa’ah, Irmi TAKAKO UTSUMI Ulfa Kholili Ummah, Fithriyah Cholifatul Ummi Maimunah Waskito, Langgeng Agung Wiwik Misaco Yuniarti Wungu, Citrawati Dyah Kencono YOSHIHIKO YANO YOSHITAKE HAYASHI Yulianti, Erikavitri Zain Budi Syulthoni Zuhrotul Eka Yulis Anggraeni