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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Sumberdaya Hayati
Fadillah, Wendi Nurul
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology

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Exploring Indonesian Sponge-Associated Marine Aspergillus hortai: Characterization of Bioactive Compounds with Potential Anti-Escherichia coli Properties Fadillah, Wendi Nurul; Sukarno, Nampiah; Iswantini, Dyah; Rahminiwati, Min; Franco, Christopher MM; Zhang, Wei; Hanif, Novriyandi; Waite, Mashuri
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.712-724

Abstract

Sponge-associated marine fungi are potential source for secondary metabolite compounds. The aim of this research was to investigate sponge-associated marine fungus as secondary metabolite producers against Escherichia coli. The fungus was isolated from Indonesian marine sponge Stylissa sp. and identified as Aspergillus hortai through a combination of morphological and molecular characteristics of ITS DNA and β-tubulin genes. The fungus was tested against E. coli using fungal broth and mycelial extracts. The optimized condition was achieved by fungal broth grown in corn meal broth at 6-days of shaking incubation. Fungal extract was produced using three liters of filtered fungal broth and extracted in ethyl acetate. The antibiotic activity of the extract is vulnerable to 45°C heat and basic or acidic conditions. Therefore, the extraction was done at pH 7 with evaporation at 40°C. The extract shows 7 major bands on TLC with 1 band shows activity against E. coli (Rf 0.81) on bioautogram. The band was observed as a yellow color and turned black in short-wave UV and did not show any fluorescence in long-wave UV. This research shows that sponge-associated marine fungi obtained from Indonesia has the potential as anti E. coli worth to be explored for searching new antibiotics.
The Effects of Root Endophytic and Arbuscular Mycorrhizal Fungi on Growth and Curcumin Content of Temulawak (Curcuma xanthorrhiza) : The Effects of Root Endophytic and Arbuscular Mycorrhizal Fungi on Growth and Curcumin Content of Temulawak (Curcuma xanthorrhiza) Putra, Sukma Triperdana; Iswantini, Dyah; Widyastuti, Utut; Fadillah, Wendi Nurul; Sukarno, Nampiah
Jurnal Sumberdaya Hayati Vol. 8 No. 2 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.2.49-56

Abstract

Temulawak (Curcuma xanthorrhiza) is known to be used as ingredient in Jamu and traditionally utilized to cure a range of illness. The global market demand for the rhizome of the temulawak affecting the urgency of sustainable rizhome production. The aim of this research was to analyze the effects of root endophytic and arbuscular mycorrhizal fungi on growth and rhizome curcumin content of the temulawak. There were five fungal inoculation treatments on C. xanthorrhiza seedling grown in sterilized and unsterilized growth media. They were root endophytic fungi A. niger (A), Glomus sp. (G), combination of A. niger and AMF Glomus sp. applied at the same time (GA) and at different time (G-A) and control. The experiment was conducted under greenhouse condition. The plant growth parameters, fungal colonization, and rhizomes curcumin content were measured. The result showed that there was a significant interaction between fungal inoculation and control treatments. In general, fungal inoculation on both sterilized and unsterilized growth media increased the plant growth and rhizomes curcumin content. On the sterilized growth media, C. xanthorrhiza inoculated by A. niger showed the best growth parameter. On the other hand, the best growth parameter on the unsterilized media was showed by C. xanthorrhiza inoculated by mixed fungal inoculums. The highest amount of curcumin content was showed by C. xanthorrhiza inoculated by Glomus sp. as a single inoculation.
Isolasi, Identifikasi, dan Produksi Miselia Rhizopus sp. Berkadar Asam Nukleat Rendah untuk Pengembangan Mikoprotein Firmansyah, Riza; Sukarno, Nampiah; Suharsono, Utut Widyastuti; Sukarno, Sukarno; Fadillah, Wendi Nurul
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 2 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.2.179

Abstract

Fungi, including Rhizopus sp., are food sources that commonly contain high nucleic acid levels. Therefore, the nucleic acid content must be reduced to achieve health standard requirements. This study aimed to isolate, identify, and produce Rhizopus sp. mycelium containing low nucleic acid. The Rhizopus spp. were isolated from tempeh collected from 12 different areas in Indonesia. Fungal identification was conducted based on morphological characteristics. The fungal isolates were selected based on mycelial growth and spore production on PDA. Biomass production of mycelium was carried out in potato extract and soybean extract media obtained from 200 g/L and 333.3 g/L, respectively. In each medium, 6 sugar levels were added, namely 0, 2, 3, 4, 5, and 6 g/L. Mycelium nucleic acid content reduction was achieved by heat treatment at 50°C and 60°C for 15 minutes and measured by a spectrophotometer at 260 nm. Fifty-eight isolates that were identified into 3 species were obtained in this experiment: R. oryzae, R. stolonifera, and R. microsporus. R. Microsporus had higher mycelium biomass and lower spore number than the other species. R. Microsporus produced a higher mycelium biomass in the soybean extract medium with 5 g/L additional sugar. The nucleic acid content of the 50°C heat-treated mycelium was 1.82% and 1.73% at 65°C. These values fulfilled the standard of mycelial nucleic acid content permitted in food by the USDA. Keywords: morphology, Rhizopus microspores, spore, soybean extract, tempeh
Co-Authors Dyah Iswantini Franco, Christopher MM M. Sukarno Nampiah Sukarno Novriyandi Hanif Putra, Sukma Triperdana Rahminiwati, Min Riza Firmansyah UTUT WIDYASTUTI Utut Widyastuti Suharsono Waite, Mashuri Wei Zhang