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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Anggraini, Nurlita Putri
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Engineering Library & Information Science

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Journal : Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan

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Biodegradation activities of microplastic polymers by Agelas conifera spongesymbiotic bacteria Ismet, Meutia Samira; Srimariana, Endang Sunarwati; Cahyati, Nur Alam Dwi; Anggraini, Nurlita Putri; Simbolon, Lenni Mariana; Cakasana, Nadya; Sari, Anita Edya Melliana; Rafi, Mohamad
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 14, No 2 (2025): JUNE 2025
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.14.2.35939

Abstract

Research on microplastic (MP) accumulation in marine organisms' tissue have been widely conducted, as well as research on microplastic biodegradation by microbial organisms from the environment. Agelas conifera sponge is proven to accumulate MP in its tissue as well as having bacteria as symbiotic organism. This research aims to screen and study biodegradation activities of A. conifera symbiotic bacteria on MP polymers, also to identify the species of those bacteria isolates. Bacterial Isolation of A. conifera sponge tissue was using Zobell 2216E agar media. Screening and biodegradation assay of the bacteria isolates was conducted on Marine Salt Media agar that was added with 0,5 g of MP polymers, using Kroc-Boral method. Diameter of clear (bacterial growth) zone surrounding Kroc-Boral indicated the biodegradation activity of the bacterial isolates. Statistical analysis on the activity was using Two Way ANOVA. Results showed that there were 9 bacterial isolates of 53 that could degrade several MP polymers, with different activities (ANOVA, p value 0.05). High and low-density polymers (Linear- Low Density PolyEthylene/ LLDPE, Polyethylene/PE, and Polyvinyl Chloride/ PVC) were found to be degraded by all 9 isolates. Meanwhile, Polyethylen Taraphtalene (PET) and Polystyrene (PS) only degraded by 8 and 3 isolates, respectively. There are only 5 isolates that have high biodegradation index (1.5) on different polymers, which mostly on PET polymer. Isolate Ac8MP found to be the most bacteria that have degradation index on all polymers more than 1. This research proves that symbiotic bacteria could have a biodegradation mechanism on MP polymers that accumulates by sponge-host. However, further study needs to be done to understand the mechanisms and effectiveness of sponge-bacterial biodegradation activity.Keywords:Dumbo catfishLeukocytesErythrocytesGlucoseMusa AcuminataAeromonas hydrophila
Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments Maysaroh, Siti; Ismet, Meutia Samira; Subhan, Beginer; Andini, Rita; Sembiring, Enny Rimita; Anggraini, Nurlita Putri
Depik Jurnal Ilmu Ilmu Perairan, Pesisir, dan Perikanan Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.32850

Abstract

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column
Co-Authors Beginer Subhan Cahyati, Nur Alam Dwi Endang Sunarwati Srimariana Hadadi, Namira Hawis H Madduppa Imanditya, Muhammad Fachriza M. Rafi Mahardika Rizqi Himawan Majariana Krisanti Meutia Samira Ismet Nadya Cakasana NEVIATY PUTRI ZAMANI Nyoman M N Natih RITA ANDINI Sari, Anita Edya Melliana Sembiring, Enny Rimita Shita Azaraly, Irma Simbolon, Lenni Mariana Siti Maysaroh