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All Journal Jurnal Arsitektur, Sains Bangunan, Kota dan Pemukiman Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine Ulil Albab
Siti Maysaroh
IPB University
Religion Arts Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Industrial & Manufacturing Engineering Social Sciences

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High and Low Taxa Specificity of Rhizosphere Bacteria Communities of Mangrove (Rhizophora mucronata) from Kuala Langsa and Telaga Tujuh Island, East Aceh Maysaroh, Siti; Samira Ismet, Meutia; Andini, Rita; Anggraini, Nurlita; Subhan, Beginer
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.55882

Abstract

Highlight Research Bacteria from mangrove sediment have been identified, and environmental parameters have been analyzed. The relationship between rhizosphere bacteria and the environment has been analyzed. The distribution and abundance of rhizosphere bacteria differ between Kuala Langsa and Telaga Tujuh. The diversity and abundance of rhizosphere bacteria are not influenced by environmental parameters. Abstract The focus of this study on understanding the structure and dynamics of rhizosphere bacteria in mangrove ecosystems is driven by the increasing acknowledgment of the crucial roles these microorganisms play in ecosystem functioning. Rhizophora mucronata, a key mangrove species, is known for its ecological significance. Investigating the bacteria associated with its rhizosphere offers valuable information about the symbiotic relationships between mangrove vegetation and microbial communities. Bacteria are vital for decomposition and nutrient availability in mangroves. This research examines sediment from rehabilitated and natural areas to understand how human and natural factors shape bacterial communities.The DNA sequence was analyzed using Next-Generation Sequencing (NGS), which targeted the 16S bacterial region in the V3-V4 rDNA. Additionally, environmental factors such as nitrate, phosphate, and sulfur content were also analyzed. Kruskal-Wallis and T-test statistical analyses were used to examine the abundance of bacteria and environmental parameters between study sites.There are differences in the types of bacteria found in Kuala Langsa and Telaga Tujuh. Approximately 7% of the rhizosphere bacterial groups were exclusively detected in Telaga Tujuh, such as Fusobacteriia (Class). Additionally, the abundance of bacteria at both locations differs significantly (p < 0.05), as determined by Kruskal-Wallis. The results of the t-test indicate that the observed environmental parameters do not differ significantly from each other. The environmental parameters studied did not significantly impact the types or abundance of detected rhizosphere bacteria.
TOROSIAJE MANGROVE ECOPARK DI KABUPATEN POHUWATO DENGAN PENDEKATAN ARSITEKTUR EKOLOGI Maysaroh, Siti; Siola, Amru; Eka, Rahmawati
Venustas Vol 2 No 2 (2023): Mei
Publisher : Program Studi Teknik Arsitektur, Fakultas Teknik - Universitas Ichsan Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37195/venustashome.v2i2.423

Abstract

Perancangan  ini bertujuan untuk merancang sebuah wisata mangrove ecopark yang  ada  di  Torosiaje Kabupaten Pohuwato  dengan  menggunakan sebuah  pendekatan Arsitektur Ekologi.   Wisata merupakan sebuah kegiatan yang dilakukan seseorang atau sekelompok orang untuk melepas penat atau berekreasi dan bersantai. Wisata juga menjadi salah satu kegiatan yang dapat membantu peningkatan pendapatan daerah. Adapun penelitian pada perancangan ini dilakukan dengan cara observasi, dokumentasi dan studi kepustakaan. Dari hasil data-data yang ada  kemudian menghasilkan sebuah rancangan kawasan wisata mangrove. Adapun tahap yang dilakukan sebelum perancangan yaitu pembuatan konsep pendekatan yang di gunakan agar kemudian akan menghasilkan sebuah gambar perancangan Mangrove Ecopark. Adapun pendekatan yang di gunakan yaitu pendekatan ekologi yang bertujuan agar pembangunan Ecopark tidak merusak lingkungan dan menggunakan material yang dapat di perbaharui sehingga tidak menyebabkan pencemaran pada lingkungan. Penerapan Arsitektur Ekologi pada perancangan terletak pada penataan bangunan yang berada di antara pepohonan mangrovesehingga nantinya tidak merusak kawasan mangrove yang ada.
Analisis Kepemimpinan Transformasional Kepala Sekolah Berdasarkan Teori Perilaku Organisasi: Studi Kasus di SMP Negeri 5 Kupang Akbar Falah Tantri; Rusli Rusli; Desilina Arif; Brigitha Maurrenzha Leatemia; Chandika Lestariaji; Siti Maysaroh
ULIL ALBAB : Jurnal Ilmiah Multidisiplin Vol. 5 No. 4: Maret 2026
Publisher : CV. Ulil Albab Corp

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56799/jim.v5i4.15420

Abstract

Kinerja guru merupakan faktor penting dalam menentukan mutu dan keberhasilan penyelenggaraan pendidikan di sekolah. Kinerja tersebut dipengaruhi oleh berbagai faktor organisasi, salah satunya adalah gaya kepemimpinan kepala sekolah. Penelitian ini bertujuan untuk menganalisis gaya kepemimpinan transformasional kepala sekolah di SMP Negeri 5 Kupang dalam perspektif Teori Perilaku Organisasi. Penelitian ini menggunakan pendekatan kuantitatif dengan metode survei. Data dikumpulkan melalui kuesioner berbasis skala Likert yang disebarkan kepada 56 guru sebagai responden. Analisis data dilakukan secara deskriptif statistik untuk mengetahui gambaran penerapan kepemimpinan transformasional kepala sekolah. Hasil penelitian menunjukkan bahwa gaya kepemimpinan transformasional kepala sekolah berada pada kategori sangat tinggi dengan nilai rata-rata keseluruhan sebesar 4,57. Kepala sekolah dinilai sangat mampu memotivasi dan menginspirasi guru, memberikan perhatian individual, mendorong kreativitas dan inovasi, serta menunjukkan keteladanan dalam disiplin dan integritas. Temuan ini sejalan dengan teori kepemimpinan transformasional Bass dan Avolio yang menekankan dimensi idealized influence, inspirational motivation, intellectual stimulation, dan individualized consideration. Penelitian ini menyimpulkan bahwa kepemimpinan transformasional kepala sekolah berperan strategis dalam menciptakan iklim kerja yang kondusif dan mendukung peningkatan kinerja guru.
Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments Siti Maysaroh; Meutia Samira Ismet; Beginer Subhan; Rita Andini; Enny Rimita Sembiring; Nurlita Putri Anggraini
Depik Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.32850

Abstract

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column
Effective DNA extraction method for metagenomic analysis of rhizosphere bacteria from mangrove sediments Siti Maysaroh; Meutia Samira Ismet; Beginer Subhan; Rita Andini; Enny Rimita Sembiring; Nurlita Putri Anggraini
Depik Vol 12, No 2 (2023): AUGUST 2023
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.32850

Abstract

Mangrove, Rhizophora mucronata, grows in the intertidal area, which contains much organic matter and varying salinity. The organic matter content is influenced by the bacterial community that inhabits the ecosystem, but information regarding the bacterial community, especially in the mangrove root system, is not widely available. There are several challenges in completing this information, one of which is that the method used is still in a conventional form. Developments in environmental DNA analysis can support and complement this information. However, this method must be optimized because the organic matter content and salt variations affect the extraction results. Thus, this study aimed to determine the optimal approach for extracting bacterial DNA from mangrove sediments. The analysis used two methodologies: manual DNA extraction techniques based on buffer modification and DNA extraction kits. There were four different treatments, namely the soil DNA isolation plus kit (M1), the fecal / soil microbial quick-DNA miniprep kit (M2), glass powder with charcoal (M3), and glass powder with skimmed milk (M4). DNA samples were obtained from each method and assessed for concentration and purity using a nanodrop. In addition, the resulting DNA's quality was analyzed using 1.5% agarose. The results obtained were in the M2 treatment, which showed optimal results compared to the others. M2 uses a bead-based beating and spin column method to achieve optimal DNA concentration through high molecular weight. The DNA obtained was also protein-free, and several samples were contaminated with humic acid, namely KL.S1, KL.S4, and T7.S4.Keywords:Bacteria 16SBead beatingDNA ExtractionSedimentSpin column
Co-Authors Amru Siola Anggraini, Nurlita Beginer Subhan Chandika Lestariaji Desilina Arif Eka, Rahmawati Enny Rimita Sembiring Hairunnisa Leatemia, Brigitha M Melati Meutia Samira Ismet MUHAMMAD ALI Nurlita Putri Anggraini RITA ANDINI Rita Andini Rusli Rusli Samira Ismet, Meutia Tantri, Akbar Falah Yetti Elfina