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Potential and Characterization of Yeast from Soil Contaminated with Captan – Based Fungicide Putri, Ratna Eka Sari; Fatiha, Chosa Zahro; Putri, Novia Dwi
G-Tech: Jurnal Teknologi Terapan Vol 9 No 3 (2025): G-Tech, Vol. 9 No. 3 July 2025
Publisher : Universitas Islam Raden Rahmat, Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70609/g-tech.v9i3.7253

Abstract

Captan is a fungicide that inhibits the growth of fungi such as Alternaria porri. This fungicide is non-systemic, forming a layer on the surface of leaves and fruit. The continuous use of captan fungicides can leave residues in the environment, especially in soil. One technique to reduce captan fungicide residues is bioremediation. Bioremediation is a way to remove fungicide residues by utilizing microorganisms. The purpose of the study was to obtain yeasts in land polluted with residues of fungicides made from active captan, knowing yeasts that are adaptive to various fungicide concentrations and have the potential to reduce the toxicity of fungicides made from active captan. This study includes soil sampling, isolation and characterization of yeasts morphologically and molecularly, yeast adaptation tests to fungicides, and degradation tests using a complete randomized design. In the adaptation test, all yeasts were able to grow on media added with various concentrations of fungicide product recommendations. Molecular identification of yeast includes DNA isolation, DNA amplification, PCR product sequencing, and molecular data analysis. In the treatment with the addition of yeast, the largest average diameter of A. porri on the seventh day of observation was in the treatment with the addition of W. anomalus and W. onychis yeast. The yeast with the highest ability to degrade fungicides containing the active ingredient captan is W. onychis.
Increase in Growth N, P, and K Absorption and Production of Corn (Zea mays L.) on Rainfed Paddy Fields Through Application of NPK 16-16-16 Fertilizer Fatiha, Chosa Zahro; Suntari , Retno; Putri, Ratna Eka Sari
G-Tech: Jurnal Teknologi Terapan Vol 9 No 3 (2025): G-Tech, Vol. 9 No. 3 July 2025
Publisher : Universitas Islam Raden Rahmat, Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70609/g-tech.v9i3.7270

Abstract

One of the national strategic foodstuffs is maize, whose production can still be increased because the actual yield in the lower than the potential yield. Problems that often arise is low fertility, especially nutrients N, P, and K. The aims of this research is to know the effect of NPK 16:16:16 fertilizer application on the growth, production and uptake of corn plants. The research was conducted in Mojokerto rainfed paddy fields with a randomized group design. The treatments given are 8 in the form of different doses of NPK 16:16:16 fertilizer and control in the form of no fertilizer and NPK 15:15:15 according to the recommended dose. Observation parameters observed were soil analysis, number of leaves, plant height, plant dry weight, NPK nutrient uptake and weight of 100 grains. Data analysis used ANOVA f test at 5% level and DMRT further test at 5% level. The application of NPK 16-16-16 fertilizer at 1/4 dose significantly increased the growth of corn plant and nutrient uptake. NPK 16:16:16 fertilizer at 1/2 dose significantly increased production.