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All Journal Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Tropical Animal Science Journal
R. I. Arifiantini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Veterinary

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Journal : Tropical Animal Science Journal

 1 
Comparison of Different Lecithin Diluents for Cryopreservation of Toraya Buffalo Semen Riwu, R. M. J.; Arifiantini, R. I.; Karja, N. W. K.
Tropical Animal Science Journal Vol. 46 No. 4 (2023): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2023.46.4.396

Abstract

The objective of this study was to compare the quality of frozen semen from Toraya buffalo with different lecithins from different commercial diluents. Fresh semen from two 6-9 years old Toraya buffaloes were collected once a week in the morning through an artificial vagina. Fresh semen was examined macroscopically and microscopically. Semen with more than 70% sperm motility was divided into three tubes, each diluted with Andromed (soy lecithin), Steridyl (egg yolk lecithin), and Bovifree (synthetic lecithin) diluents at a concentration of 100 x106 mL-1 motile sperm. The diluted sperm were placed in 0.25 mL straws and allowed to equilibrate for 4 hours. The sperm was frozen above liquid nitrogen favour for 15 minutes and stored in liquid nitrogen containers for further evaluation. The quality of frozen semen was assessed 24 hours after freezing. The parameters tested were motility, viability, abnormalities, plasma membrane integrity, and sperm recovery rate (RR). The results showed no significant differences in the values of motility, viability, abnormalities, plasma membrane integrity, and sperm RR in the three diluents used. Sperm motility values ranged from 45.93% to 47.09% after freezing. Sperm viability ranged from 56.21% to 60.27%. The values of membrane integrity of the three diluents used ranged from 57.73% to 61.36%. The values of sperm abnormalities after freezing and thawing ranged from 2.74% to 3.18%. In conclusion, three commercial diluents containing animal, vegetable, and synthetic lecithin bases can be used as diluents for freezing Toraya buffalo semen with similar results.
Variations in Semen Quality and Potential for Frozen Semen Production in Aceh Cattle Sophian, E.; Said, S.; Setiadi, M. A.; Arifiantini, R. I.
Tropical Animal Science Journal Vol. 48 No. 1 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.1.1

Abstract

The quality of fresh and frozen semen as well as the potential to produce frozen semen, vary considerably between individual animals and cattle breeds. This study aimed to analyze the quality of fresh and frozen semen in Aceh cattle and calculate the potential production of frozen semen in Aceh bulls over 1 year. This study used primary data on the quality of fresh and frozen semen from five Aceh cattle and obtained secondary data from artificial insemination centers in Lembang and Singosari in 2022. Semen samples were collected weekly, and the quality of fresh and frozen semen samples were evaluated. The data were analyzed using a one-way analysis of variance at 95% significance level, followed by Tukey’s test. The results revealed differences in semen volume and pH. Based on microscopic characteristics, the semen samples from different bulls exhibited no discernible differences in mass movement, sperm motility, viability, morphology, and plasma membrane integrity, except for sperm concentration and acrosomal integrity. Bull 211605 exhibited the highest sperm concentration. Furthermore, the frozen semen samples from Aceh cattle showed no significant differences in viability, plasma membrane integrity, morphology, and acrosomal integrity. The total sperm motility of bull 211710 was higher than that of the other bulls. The highest progressive motility was observed in bulls 211710 and 211605, and the highest intact DNA was detected in bulls 211710 and 211605. The results of this study demonstrated that the quality of fresh and frozen semen in Aceh cattle is distinct, and the potential frozen semen production of Aceh cattle is estimated to range from 3,382 ± 1810 to 11,399 ± 2658 straws/year.
Co-Authors Amal, A. S. Arif, A. A. Ariyanti, R. B. Purwantara B. Purwantara C. SUMANTRI Darussalam, I. Eros Sukmawati Hasibuan, H. Herbowo, M. T. I. Supriatna Indriastuti, R. Khaeruddin Khaeruddin M. A. Setiadi, M. A. N. W. K. Karja, N. W. K. Rasad, R. S. D. Riwu, R. M. J. Rosyada, Z. N. A. S. Darwati S. Said Setiadi, D. R. Sianturi, R. G. Sophian, E. T. Maulana, T. T. Suryati Ulupi, N.