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T. Maulana, T.
Research Centre for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46 Cibinong 16911
Agriculture, Biological Sciences & Forestry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Energy Engineering Health Professions Law, Crime, Criminology & Criminal Justice Social Sciences

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STUDY ON CHANGES OF SPERM HEAD MORPHOMETRY AND DNA INTEGRITY OF FREEZE-DRIED BOVINE SPERMATOZOA Said, S.; Afiati, F.; Maulana, T.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 3 (2015): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.3.145-152

Abstract

Changes of sperm heads morphometric and DNA integrity of freeze-dried bovine spermatozoa were investigated. Freeze-dried spermatozoa had stored in the refrigerator at 4°C for 2 years. Computer-assisted sperm analysis (CASA) was used in this study to identify sperm head morphometry, while for DNA integrity analysis using acridine orange staining. Samples were smeared on glass slides, fixed for 2 h in acetic alcohol and stained with acridine orange solution. After staining, each slide was examined at x400 magnification in a fluorescence microscope with axio vision (Zeiss Company, Germany). Proportion of fluorescence red and green emissions of the sperm head were examined and scored. These results indicated that sperm head had enlarged significantly (P<0.05) after freeze-drying process. However, freeze-dried sperm heads morphometry significantly (P<0.05) decrease after incubation for 3 and 6 hours. Changes of DNA integrity of freeze-dried spermatozoa significantly (P<0.05) decrease after incubation for 6 hours. In the present study concluded that (1) freeze-drying spermatozoa caused sperm head morphometric enlarged, whereas incubation time caused sperm heads decreased, (2) DNA integrity of freeze-dried sperm head is still intact during incubation 3 hours, and decreased DNA integrity occur in incubation for 6 hours.
Effect of addition an amino acid or its combination with EDTA on DNA integrity and morphometry sperm heads of freeze-dried bovine spermatozoa Said, S.; Maulana, T.; Setiorini, S.; Ibrahim, G.E.; Ramadhan, M.N.; Christopher, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 3 (2020): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.3.189-196

Abstract

The objective of the current study was to investigate the effect of addition an amino acid or its combination with EDTA on DNA integrity and morphometry sperm heads of freeze-dried bovine spermatozoa. Spermatozoa were freeze-dried in medium with the addition of an amino acid glycine, cysteine, glutamine, or its combination with EDTA. Sperm head morphometry was identified at 400X magnification using Axio Vision(Zeiss Company, Germany), while for membrane plasma integrity (MPI) determined by calculation of the percentage of spermatozoa having intact plasma membrane by osmotic resistance test method and DNA integrity analysis using acridine orange staining. Sperm head had declined in size after the freeze-drying process, MPI of FD spermatozoa gradually increased significantly when FD solution was added with an amino acid solution (glycine, cysteine) and its combination with EDTA. DNA integrity of all freeze-dried spermatozoa treatments was remaining intact, no significantly different (P>0.01) among treatments. The present study concluded that the addition of an amino acid (glycine, cysteine) or its combination with EDTA could be reduced morphometric sperm heads and plasma membrane damage of freeze-dried bovine spermatozoa, however, DNA integrity of bovine sperm nucleus remaining intact after the freeze-drying process without addition both amino acids and EDTA. 
Sex sorting sperm of sumba ongole bulls by using snakehead fish (Channa striata) albumin extract Maulana, T.; Said, S.; Arifiantini, R. I.; Setiadi, M. A.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 1 (2019): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.1.106-113

Abstract

The objective of this research was to investigate the potential of snakehead albumin extract (channalbumin) for sorting X and Y sperm of Sumba Ongole (SO) and its characteristic. Semen was collected from three SO bulls using artificial vagina and the freeze dried channalbumin was extracted from snakehead fish. Channalbumin column was made with different concentration ratio of top and bottom fraction: 2%:4%; 3%:5%; 4%:6% respectively and BSA 5%:10% as control. Semen was put in top fraction then incubated for 30 min at room temperature then each fraction was centrifuged at 1800 rpm for 10 minutes. The pellet was evaluated for motility, abnormality, viability, membrane integrity and head sperm morphometric. The results showed that the channalbumin capable to maintain sperm motility in the top fraction better than the bottom fraction. Sperm viability and membrane integrity in control group were significantly higher (P<0.05) than all channalbumin treatment. BSA 5%:10% has highest proportion of X and Y sperm (69%:76.77%) compared with 2%:4% (42.33%:79.13%), 3%:5% (55.97%:75.73%) and 4%:6% of channalbumin (62.77%:68%). It’s concluded that channalbumin 4%: 6% was effective for separation of XY sperm with higher proportion.
Proteomic Analysis of Pesisir Bull Sperm in Different Age Groups for the Identification of Reproductive Function Proteins Anwar, P.; Maulana, T.; Sumantri, C.; Arifiantini, I.; Gunawan, A.
Tropical Animal Science Journal Vol. 48 No. 5 (2025): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2025.48.5.393

Abstract

Pesisir cattle are native to Indonesia and originate from West Sumatra. It is known for its high environmental adaptability and can be further developed using a proteomic approach. The proteomic approach for testing the fertility of Pesisir bulls is an important factor in determining a potentially superior breed. Proteomic studies of reproduction in Pesisir bulls have not been widely conducted. This study aimed to identify and characterize functional sperm proteins in Pesisir bulls that are associated with reproductive processes. Semen samples were collected from 12 pesisir bulls aged 2–4 years and grouped into three age categories. Protein profile analysis was performed using polyacrylamide gel electrophoresis with 1D-SDS-PAGE, followed by proteolytic digestion of the proteins in the gel and protein identification using LC-MS/MS analysis. Protein functions were predicted based on analysis of biological annotations generated using UniProt, Venny, PANTHER, and STRING. Based on the evaluation of fresh semen, sperm motility in Pesisir bulls was found to increase with age. Proteomic analysis successfully identified 334 proteins in the sperm of Pesisir bulls. Among these, two proteins, ZPBP and SPACA3, were identified as involved in reproductive and fertilisation processes. Functionally, both proteins play crucial roles in acrosomal events during fertilisation. Gene Ontology analysis showed that most proteins in sperm are involved in various biological processes, including cellular activities, metabolic processes, and molecular functions related to catalytic activities. In conclusion, SPACA3 and ZPBP proteins were identified as potentially involved in reproductive processes and may serve as fertility markers in Pesisir bulls.
Determinants of Compliance of Pregnant Women in Participating in the Class Program for Pregnant Women in Aceh Besar District Alfiana, Alfiana; Z, Nasrul; Usman, Said; Maulana, T.; Saputra, Irwan
Journal of World Science Vol. 2 No. 3 (2023): Journal of World Science
Publisher : Riviera Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58344/jws.v2i3.250

Abstract

The implementation of the pregnant women class in Aceh Besar Regency has been carried out since 2020 with a target of 11,492 pregnant women. From observations, it is known that most pregnant women are less obedient in attending maternity classes. So, the purpose of this study is to analyze the compliance of pregnant women in participating in the class program for pregnant women in Aceh Besar district. This type of research uses observational cross-sectional design. The population of this study included classes of pregnant women at Indrapuri Health Center, Kuta Baro Health Center, and Darul Imarah Health Center, the sample in this study amounted to 180 pregnant women. The study found that the R-square value for service satisfaction was 0.694 and that internal and external factors could affect the compliance variables of pregnant women by 69.4%, with the remaining 30.6% influenced by other factors. Additionally, the implementation of the pregnant women class program was found to have an R-square value of 0.667, indicating that internal and external factors could affect it by 66.7%, with the remaining 33.3% influenced by other factors. The study also identified external factors, specifically knowledge, as the primary influencing factor on the implementation of the pregnant women class program, with a calculated value of 35,768 > table 1,624. Internal factors (age, income, motivation, and parity) and external factors (knowledge, attitudes, family support, and mileage) influence the compliance of pregnant women and the implementation of classes for pregnant women in Aceh Besar District.
Integrated Metabolomic and Functional Assessment of Sexed Frozen Semen in Holstein Friesian Bulls Yusuf, M.; Diansyah, A. M.; Sahiruddin, S.; Masturi, M.; Maulana, T.; Said, S.; Rahmat, R.; Alfian, A. M.; Adam, A. A. S.; Yusri, A. N. H. S.; Nurlatifah, A.; Amrullah, M. F.
Tropical Animal Science Journal Vol. 49 No. 1 (2026): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2026.49.1.19

Abstract

This study examined the differences in sperm quality and metabolite profiles among X and Y sperm in Friesian Holstein bulls. It also tried to find specific metabolites that can help improve the accuracy of sexed semen use in humid tropical dairy farms. Semen samples from five bulls were separated by sex and tested for movement, live sperm count, shape, and membrane and acrosome health. The tests used Computer-Assisted Sperm Analysis (CASA) and regular microscope checks. Metabolite analysis was performed using LC-HRMS, followed by various statistical tests, pathway checks, and ROC curve analysis. The outcomes showed that X sperm had better movement and acrosome health, while Y sperm had stronger membranes, fewer shape problems, and moved faster. The untargeted metabolite study showed clear differences between the two groups. X sperm had more identified metabolites and greater average levels. The crucial metabolites found more in X sperm were D-(−)-fructose, L-(+)-lactic acid, and sn-glycero-3-phosphocholine. Meanwhile, Y sperm had greater levels of acetyl-L-carnitine. The pathway analysis showed that X sperm primarily utilized carbohydrate and fat processing through glycolysis and phosphocholine pathways to maintain their movement and membrane integrity. On the other hand, Y sperm mainly use a carnitine-based energy pathway to support their faster and straighter movement. The identified metabolites can serve as reliable biomarkers to aid in laboratory quality control checks and enhance the quality of semen extenders. This outcome gives a good way to make sexed semen more stable and reliable in tropical dairy farming conditions.
Co-Authors A. GUNAWAN Adam, A. A. S. Alfian, A. M. Alfiana Alfiana Amrullah, M. F. Anwar, P. C. SUMANTRI Christopher, E. Diansyah, A. M. F. Afiati, F. I. Arifiantini Ibrahim, G.E. M. A. Setiadi, M. A. M. Yusuf Masturi Masturi Nurlatifah, A. R. I. Arifiantini Rahmat, R. Ramadhan, M.N. S. Said Sahiruddin, S. Saputra, Irwan Setiorini, S. Usman, Said Yusri, A. N. H. S. Z, Nasrul