Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal Akuakultur Indonesia
. ARIFUDDIN
Unknown Affiliation
Agriculture, Biological Sciences & Forestry

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
The Effect of Hydroquinone Extracted from Sonneratia caseolaris Fruit on the Hemolymph of Tiger Prawn, Penaeus monodon Fab., Infected by Vibrio harveyi Arifuddin, .; Sukenda, .; Dana, D.
Jurnal Akuakultur Indonesia Vol. 3 No. 1 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.169 KB) | DOI: 10.19027/jai.3.23-28

Abstract

Study on the effect of hydroquinone extracted from 5. caseolaris fruit on Penaeus monodon immune system was carried out. Hydroquinone was extracted by methanol and chloroform using a soxhlet apparatus. Two kinds of in vivo experiments were conducted that were (1) shrimps were only injected with hidroquinone and (2) shrimps were injected with hidroquinone and challenged with Vibrio harveyi. Total hemocyte, differential hemocyte and phagocytic index were examined on shrimp injected with only hidroquinone and shrimp injected with hidroquinone and challenged with V. harveyi. Total hemocyte of hydroquinone injected shrimp increased significantly by day 5 from 8.33xl06 to 4,93 x 107 cells/ml. Hyaline and semi granular hemocyte cells by day 7 decreased from 61,15% to 27,84% and 8,14% to 2,53%, respectively. However, granular hemocyte cells increase significantly from 30,71% to 69,63%. Phagocytic index increased significantly by day 7 from 0.41 to 0,76. These findings indicated that crude hydroquinone can increase non spesifik imune response of P. monodon against V. harveyi infection. Key words: hydroquinone. Sonneratia caseolaris. Vibrio harveyi, Penaeus monodon, immune system   ABSTRAK Kajian tentang efek hidrokuinon yang diekstraksi dari buah S. caseolaris pada sistem imun udang windu, Penaeus monodon, dilakukan. Hidrokuinon diekstraksi dengan methanol dan kloroform. Dua jenis percobaan in vivo dilakukan yaitu (1) udang hanya diinjeksi dengan hidrokuinon (2) udang diinjeksi dengan hidrokuinon kemudian diuji tantang dengan bakteri Vibrio harveyi. Total hemosit, diferensial hemosit dan indeks pagositas diperiksa pada udang yang dinjeksi dengan hidrokuinon saja dan pada udang yang diinjeksi dengan hidrokuinon dan diuji tantang dengan bakteri V. harveyi. Total hemosit meningkat secara nyata sampai hari ke 5 dari 8,33 x I06 ke 4,93 x 107 sel/ml. Hyaline dan sel-sel semi granular sampai hari ke 7 masing-masing turun dari 61,15% ke 27,84% dan 8,14% ke 2,53%. Hasil ini menunjukkan bahwa hidrokuinon dapat meningkatkan respon imun spesifik pada udang terhadap infeksi V. harveyi. Kata kunci: hidrokuinon, Sonneratia caseolaris, Vibrio harveyi, Penaeus monodon, sistem imun
The Effect Hydroquinone Extracted from Sonneratia caseolaris Fruit to Control Vibrio harveyi Artificial Infection on Tiger Shrimp, Penaeus monodon Fab. Arifuddin, .; Sukenda, .; Dana, D.
Jurnal Akuakultur Indonesia Vol. 3 No. 1 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (260.321 KB) | DOI: 10.19027/jai.3.29-35

Abstract

The role of hydroquinone extracted from Soneratia caseolaris fruit to control Vibrio harveyi infection on tiger prawn was carried out. In vitro experiment was conducted using disc diffusion and MIC {minimum inhibitory concentration) methods to know the sensitivity of V. harveyi to hydroquinone. Two kinds of in vivo experiments were (1) hydroquinone was injected into shrimps muscle and a week later the shrimps were challenged with V. harveyi (2) the shrimps were challenged with V. harveyi and one day later hidroquinone was injected. Total count of live V. harveyi on the shrimps and survival rate were observed after challenge test. Hydroquinone showed antibacterial activity with MIC at 3000 ppm. Hydroquinone injected shrimp showed higher survival rate compared with control (100% vs 50%). Total count of V. harveyi from injected shrimp, either before or after challenged, decreased by 2,61xl04 cfu/g and l,61xl04 cfu/g, respectively. These findings indicated that crude hydroquinone have anti-bacterial effect to control V. harveyi infection. Key words: hydroquinone, Sonneratia caseolaris, Vibrio harveyi, Penaeus monodon   ABSTRAK Telaah peran hidrokuinon yang diekstraksi dari buah Soneratia caseolaris untuk mengontrol infeksi Vibrio harveyi pada udang windu dilakukan. Percobaan in vitro dilakukan menggunakan metode difusi cakram dan MIC {minimum inhibitory concentration) untuk mengetahui sensitivitas V. harveyi terhadap hidrokuinon. Percobaan in vivo dilakukan dengan dua cara (1) hidrokuinon disuntikkan pada otot udang dan seminggu kemudian udang diuji tantang dengan V. harveyi (2) udang ditantang terlebih dahulu dengan V. harveyi dan sehari kemudian hidrokuinon disuntikkan. Jumlah total bakteri V. harveyi hidup pada udang dan kelangsungan hidup udang diamati setelah uji tantang. Hidrokuinon menunjukkan aktivitas antibakteri dengan MIC 3000 ppm. Udang yang diinjeksi dengan hidrokuinon mempunyai kelangsungan hidup yang lebih tinggi dibandingkan kontrol (100% vs 50%). Jumlah total V. harveyi pada udang yang diinfeksi, baik sebelum maupun sesudah, masing-masing turun sampai 2,61 x 104 cfu/g dan 1,61 x 104 cfu/g. Hasil ini menunjukkan bahwa hidrokuinon mempunyai efek anti-bakterial untuk mengontrol infeksi V. harveyi. Kata kunci: hidrokuinon, Sonneratia caseolaris, Vibrio harveyi, Penaeus monodon
Co-Authors D. Dana Sukenda .