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All Journal HAYATI Journal of Biosciences Jurnal Pengajaran Matematika dan Ilmu Pengetahuan Alam BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan)
Any Aryani
jurusan pendidikan Biologi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Mathematics

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IDENTIFIKASI MISKONSEPSI SISWA SMP PADA KONSEP FOTOSINTESIS MELALUI ANALISIS GAMBAR Ariandini, Devi; Anggraeni, Sri; Aryani, Any
Jurnal Pengajaran Matematika dan Ilmu Pengetahuan Alam Vol 18, No 2 (2013): Jurnal Pengajaran MIPA
Publisher : Faculty of Mathematics and Science Education, Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18269/jpmipa.v18i2.36

Abstract

Tujuan dari penelitian ini adalah untuk mengidentifikasi miskonsepsi siswa dengan analisis menggambar. Tiga puluh empat siswa di kelas 8 di sekolah menengah pertama digunakan sebagai subyek dalam penelitian ini. Teknik sampel menggunakan teknik purposive sampling. Data yang diperlukan untuk penelitian ini dikumpulkan melalui kuesioner, menggambar analisis rubrik, dan wawancara. Identifikasi Kesalahpahaman ditentukan dengan menggambar siswa berdasarkan menggambar kriteria klasifikasi oleh Kose (2008). Siswa diperintahkan untuk menarik tentang konsep fotosintesis setelah proses pembelajaran. Gambar siswa dianalisis dengan rubrik dan setelah itu mereka diwawancarai. Hasil penelitian menunjukkan tidak semua tingkat kriteria menggambar dari tingkat 1 sampai 5 diidentifikasi. Dari hasil analisa, gambar paling banyak ditemukan adalah pada tingkat 4. Gambar pada tingkat 4 dikategorikan sebagai gambar lengkap konsep fotosintesis dan tidak ada kesalahpahaman ditemukan. Berdasarkan gambar mereka, ada siswa 2,9% diidentifikasi yang diselenggarakan kesalahpahaman. Ada perbedaan jumlah siswa yang diadakan kesalahpahaman antara gambar hasil analisis dan hasil wawancara. Dari hasil wawancara, ada siswa 35,2% dimiliki kesalahpahaman, lebih dari menggambar hasil analisis. Ini berarti bahwa identifikasi kesalahpahaman melalui analisis gambar tidak efektif. Kesalahpahaman siswa yang paling sering terjadi pada konsep fotosintesis adalah tempat berlangsungnya fotosintesis. Faktor yang membuat kesalahpahaman siswa yaitu karena siswa itu sendiri dan lingkungannya.Kata Kunci: fotosintesis, kesalahpahaman, konsep, menggambar analisis
Successful Primer Picking and Pooling for the Design of Multiplex PCR Primers Specific to Pork, Beef, Chicken, and Rat DNA Kusumawaty, Diah; Faridah, Nurul; Fibriani, Azzania; Priyandoko, Didik; Dzikrina, Hanina; Puspitasari, Diah; Tallei, Trina Ekawati; Aryani, Any
HAYATI Journal of Biosciences Vol. 31 No. 4 (2024): July 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.4.678-686

Abstract

DNA markers and Multiplex-PCR have emerged as methods for species detection in processed meat products. The primary objective of this study is to design multiplex primer sequences for pork, rat, beef, and chicken, generating distinguishable amplicons through agarose gel electrophoresis for halal detection in processed meat products. Primer design involved utilizing mitochondrial genomic data and the NCBI-Primer BLAST site to obtain specific pork and beef primer sequences. In silico simulations, including single and multiplex-PCR, were conducted using Primer Pooler. In vitro validation encompassed Single-PCR and Multiplex-PCR annealing temperature optimization, using samples of chicken, beef, pork, and rat as well as processed meat products like meatballs, sausages, and nuggets. In vitro validation demonstrated that the halal marker gene's multiplex primer efficiently amplified the target sequence, specifically at the optimal annealing temperature of 58°C. Amplicons from beef (1,217 bp), pork (860 bp), rat (622 bp), and chicken (272 bp) primers could be distinguished on a 1.5% agarose gel. The study's results can aid in cost-effective and rapid halal testing and authentication of processed meat products, offering advantages over PCR with a single primer.
VIRULENCE AND HORIZONTAL TRANSMISSION POTENTIAL OF ENTOMOPATHOGENIC FUNGI AGAINST SUBTERRANEAN TERMITES (Macrotermes gilvus) Harnieyane, Dendu Noutera Pramayuditya; Peristiwati, Peristiwati; Aryani, Any
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 2 (2026): Biolink February
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i2.15380

Abstract

Indonesia’s tropical climate provides favorable conditions for subterranean termites, particularly Macrotermes gilvus, which cause significant economic losses as wood-destroying pests. Chemical control methods may lead to resistance and environmental problems; therefore, alternative control strategies are needed. This study aimed to evaluate the virulence of Metarhizium sp., Beauveria sp., Aspergillus sp., and Penicillium sp. against M. gilvus based on termite mortality, wood weight loss, and horizontal transmission. Isolates of Metarhizium sp. and Beauveria sp. were obtained from the BSIP Lembang laboratory collection, while Aspergillus sp. and Penicillium sp. were isolated from rhizosphere soil at the same site. The experiment used 13 treatments with three replications, consisting of three conidial concentrations (10⁶, 10⁷, and 10⁸ conidia/ml) and a control. Bioassays were conducted at 26–28 °C and 70–95% relative humidity using no-choice termiticide and horizontal transmission tests. Data were analyzed using the Kruskal–Wallis test. All fungal isolates caused termite mortality and wood weight loss. Beauveria sp. at 10⁸ conidia/ml showed the highest efficacy, achieving 100% mortality within 24 hours. Horizontal transmission was indicated by white hyphal growth on termite bodies. These results demonstrate that entomopathogenic fungi, particularly Beauveria sp., are promising environmentally friendly biological control agents against M. gilvus.
Co-Authors Azzania Fibriani Devi Ariandini Diah - Kusumawaty Diah Puspitasari Didik Priyandoko Dzikrina, Hanina Harnieyane, Dendu Noutera Pramayuditya Nurul Faridah, Nurul Peristiwati, Peristiwati Sri Anggraeni TRINA EKAWATI TALLEI