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SIMULTANEOUS PATHOGEN DETECTION OF SHRIMP VIRUSES ON CULTURED TIGER SHRIMPS (Penaeus monodon) IN INDONESIA Koesharyani, Isti; Sudaryatma, Putu Eka; Gardenia, Lila; Aryati, Yani; Mahardika, Ketut; Mufidah, Tatik
Indonesian Aquaculture Journal Vol 18, No 1 (2023): (June, 2023)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.18.1.2023.79-86

Abstract

The multiple-pathogen infection causes severe economic impact to shrimp industry in Indonesia and worldwide due to mass mortality and multiple abnormalities of the survived infected shrimps. However, multiple-pathogen detection tools in shrimp diseases have not yet widely used. The purpose in this study was to develop and applied simultaneous detection system using multiplex polymerase chain reaction (PCR) assay from natural infections caused by white spot syndrome virus (WSSV), infectious hypodermal and haematopoietic necrosis virus (IHHNV) and monodon baculovirus (MBV) in Black tiger shrimp culture. To analyze multiple-pathogen infections in the shrimp, the study designed and used three pairs of specific primers targeting DNA virus from the shrimp diseases. All amplifications used a specific master mix for multiplex PCR assay and standardized extracted nucleic acid from the samples. This mPCR assay successfully amplified the DNA of three viruses in a single tube-run by multiplex PCR for each virus. Based on the results, the study confirms that multiple-pathogen infection contributes the highest mass mortality rather than from single infection by either WSSV, IHHNV or MBV. This study also confirms that the mPCR assay is a faster, cheaper, and efficient method to detect and subsequently prevent the spreading of multi-pathogen shrimp diseases.
The effectiveness of a combination of probiotics and natural wheat and sesame based prebiotics in enhancing the growth performance of giant freshwater prawns (Macrobrachium rosenbergii) Khairunnisa, Maharani; Farastuti, Eko Rini; Darmarini, Ananingtyas S; Aryati, Yani
JURNAL MINA SAINS Vol. 12 No. 1 (2026): Jurnal Mina Sains
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jmss.v12i1.19233

Abstract

The main challenge in farming M. rosenbergii is its relatively slow growth rate, which directly affects productivity and profitability. This research evaluates the effectiveness of probiotic and prebiotic combinations based on wheat and sesame (synbiotics) on the growth performance of giant prawns. This research used a Completely Randomized Design (CRD) with four treatments: Control (P1), Probiotic (P2), Wheat-based Synbiotic (P3), and Sesame-based Synbiotic (P4), each replicated three times. Observed parameters included growth performance, feed conversion ratio (FCR), survival rate, and intestinal histology. Treatment P3 provided the best result with an average weight gain of 4.03 ± 1.85 g, a specific growth rate (SGR) of 0.61 ± 0.23% per day, a length gain of 1.82 ± 0.45 cm, and a specific growth rate of length (SLGR) of 0.27 ± 0.06% per day. The lowest Feed Conversion Ratio (FCR) was found in P3, at 2.66 ± 0.53 g, indicating the best feed efficiency and more uniform and longer intestinal microvilli. The survival rate in P3 reached 93.33 ± 11.55%. Treatment P3 improved growth, feed efficiency, and intestinal health in giant prawns. Additional studies are suggested to investigate the molecular mechanisms by which synbiotics affect digestive and immune-related genes.