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All Journal HAYATI Journal of Biosciences Indonesian Aquaculture Journal Journal of Fisheries & Marine
Lila Gardenia
Pusat Penelitian dan Pengembangan Perikanan Budidaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences

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SIMULTANEOUS PATHOGEN DETECTION OF SHRIMP VIRUSES ON CULTURED TIGER SHRIMPS (Penaeus monodon) IN INDONESIA Koesharyani, Isti; Sudaryatma, Putu Eka; Gardenia, Lila; Aryati, Yani; Mahardika, Ketut; Mufidah, Tatik
Indonesian Aquaculture Journal Vol 18, No 1 (2023): (June, 2023)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.18.1.2023.79-86

Abstract

The multiple-pathogen infection causes severe economic impact to shrimp industry in Indonesia and worldwide due to mass mortality and multiple abnormalities of the survived infected shrimps. However, multiple-pathogen detection tools in shrimp diseases have not yet widely used. The purpose in this study was to develop and applied simultaneous detection system using multiplex polymerase chain reaction (PCR) assay from natural infections caused by white spot syndrome virus (WSSV), infectious hypodermal and haematopoietic necrosis virus (IHHNV) and monodon baculovirus (MBV) in Black tiger shrimp culture. To analyze multiple-pathogen infections in the shrimp, the study designed and used three pairs of specific primers targeting DNA virus from the shrimp diseases. All amplifications used a specific master mix for multiplex PCR assay and standardized extracted nucleic acid from the samples. This mPCR assay successfully amplified the DNA of three viruses in a single tube-run by multiplex PCR for each virus. Based on the results, the study confirms that multiple-pathogen infection contributes the highest mass mortality rather than from single infection by either WSSV, IHHNV or MBV. This study also confirms that the mPCR assay is a faster, cheaper, and efficient method to detect and subsequently prevent the spreading of multi-pathogen shrimp diseases.
Isolation and Identification of Vibrio parahaemolyticus Bacteria in Bottlenose Dolphins (Tursiop truncates) in Kendal Conservation Pond, Central Java Nafiqoh, Nunak; Setiadi, Setiadi; Novita, Hessy; Lusiastuti, Angela Mariana; Indrawati, Agustin; Elmanaviean; Jannah, Siti Nur; Gardenia, Lila; Penataseputro, Tanjung; Andriyono, Sapto; Ningrum, Siti Gusti; Oktaviani, Dian; Syahidah, Dewi; Wattiheluw, Muhammad Subhan; Purwaningsih, Uni
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.811-819

Abstract

Aquatic mammals in Indonesia are officially protected. However, there is a lack of research on these mammals, particularly in relation to potential disease-causing bacteria. A study was conducted in a conservation pond to address this gap, where swab samples were taken from the blowholes of aquatic mammals. The focus was on identifying bacteria that could potentially cause infectious diseases in these animals. The results revealed V. parahaemolyticus bacterial isolates, which showed a 98% similarity to the registered V. parahaemolyticus in NCBI. These bacterial isolates exhibited hemolysin properties and demonstrated resistance to trimethoprim, streptomycin, cephalothin, and penicillin antibiotics.
In silico and In vitro Antibacterial Activity of Centella asiatica Leaves Bioactive Compounds Against Aquaculture Pathogenic Bacteria Andriyanto, Septyan; Maftuch, Maftuch; Andayani, Sri; Nafiqoh, Nunak; Gardenia, Lila; Novita, Hessy; Nursid, Muhammad
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 3 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i3.72072

Abstract

Graphical Abstract     Highlight Research The GC-MS analysis of the asiatica leaves extracts identified 53 bioactive compounds. The crude extracts of asiatica showed antibacterial efficacy against fish pathogenic bacteria. 13-Hexyloxacyclotridec-10-en-2-one has the potential to be an inhibitor of DNA gyrase. Bioactive compounds derived from C. asiatica leaves extracts show potential as antibacterial agents.     Abstract Antimicrobial agents are crucial for managing bacterial infections in fish cultures. Centella asiatica is a medicinal plant recognised for its diverse bioactive compounds with important antibacterial properties. The present study aimed to investigate the antibacterial activity of C. asiatica leaves bioactive compounds on fish pathogenic bacteria using an in vitro and in silico approach. The maceration method was used to extract bioactive compounds from C. asiatica leaves and was identified using Gas Chromatography-Mass Spectrometry (GC-MS). In vitro analysis of antibacterial activity was evaluated using the minimum inhibitory concentration method. While in silico molecular docking is applied alongside assessing Lipinski's rules of five, as well as absorption, distribution, metabolism, excretion, and toxicity properties. The result of the GC-MS examination of the C. asiatica leaf extracts identified 53 bioactive compounds. In vitro studies showed antibacterial efficacy of leaf extracts against fish pathogenic bacteria (Streptococcus agalactiae, Bacillus subtilis, and Staphylococcus aureus) with minimum inhibitory concentration values of 12,5 mg/ml. In silico molecular docking analysis showed that several bioactive compounds have the potential to be DNA gyrase inhibitors. Compound 13-Hexyloxacyclotridec-10-en-2-one has the highest inhibition with binding energy of −7,4 Kcal/mol compared to ciprofloxacin as drug standard with a binding energy value −7,3 Kcal/mol. The following compound is gamma.-Muurolene (−6,7 Kcal/mol), Copaene (−6,6 Kcal/mol) and Humulene (−6,6 Kcal/mol). These results suggest that bioactive compounds of C. asiatica leaves extracts hold promise as potential antibacterial agents for treating fish pathogenic bacteria infections.
Co-Authors Agustin Indrawati Andriyanto, Septyan ANGELA MARIANA LUSIASTUTI Elmanaviean Isti Koesharyani Ketut Mahardika Maftuch Maftuch Mufidah, Tatik Muhammad Nursid Nafiqoh, Nunak Novita, Hessy Oktaviani, Dian Penataseputro, Tanjung Putu Eka Sudaryatma Sapto Andriyono Setiadi Setiadi Siti Gusti Ningrum, Siti Gusti Siti Nur Jannah Sri Andayani Syahidah, Dewi Uni Purwaningsih Wattiheluw, Muhammad Subhan Yani Aryati