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All Journal Buitenzorg: Journal of Tropical Science
Rusydina, Diyana Sabila
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Health Professions

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Micromorphological Characterization and DNA Barcoding of Durio macrantha Kosterm. from the Bogor Botanical Garden Collection Magandhi, Mahat; Rusydina, Diyana Sabila; Mukaromah, Arnia Sari
Buitenzorg: Journal of Tropical Science Vol 2 No 1 (2025): Vol. 2 No. 1 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i1.16

Abstract

Durio macrantha is an endemic species first discovered in 1981 in Gunung Leuser National Park, North Sumatra, and has been conserved at the Bogor Botanical Garden since 1994. This study aims to characterize the micromorphology and DNA profile D. macrantha using DNA barcoding with rbcL and ITS gene markers. Sample of D. macrantha were collected from the Bogor Botanical Gardens for analysis. Micromorphological analysis revealed that the leaves exhibit three vein types (primary, secondary, and tertiary) with a pentagonal venation pattern and a tri-veinlet veinlet pattern. Additionally, eight types of trichomes were observed on the abaxial surface. Molecular characterization showed that the rbcL sequence was 563 bp long, with nucleotide composition T (27.9%), C (21.7%), A (27.9%), and G (22.6%). The ITS sequence was 960 bp long, with nucleotide composition T (15.4%), C (33.4%), A (19.0%), and G (32.2%). BLAST analysis of both rbcL and ITS genes revealed a high level of similarity between D. macrantha and D. zibethinus. This study provides fundamental data supporting the conservation and further research of D. macrantha, particularly in morphological and molecular aspects.
Co-Authors Arnia Sari Mukaromah Mahat Magandhi